MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 3786)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3500 545.8253 2179.2721
3541 732.3896 2194.1470
3542 1098.5800 2195.1454
3544 733.0904 2196.2494
3571 737.0919 2208.2539
3575 737.4261 2209.2565
3579 553.8245 2211.2689
3582 739.7592 2216.2558
3594 743.0618 2226.1636
3605 744.7480 2231.2222
3626 748.4181 2242.2325
3627 748.4193 2242.2361
3629 1122.6161 2243.2176
3637 750.3892 2248.1458
3641 751.4335 2251.2787
3642 751.4662 2251.3768
3653 754.3934 2260.1584
3666 758.4063 2272.1971
3669 758.7384 2273.1934
3684 761.4072 2281.1998
3686 762.1032 2283.2878
3687 762.7809 2285.3209
3696 766.4338 2296.2796
3698 767.4172 2299.2298
3703 576.3122 2301.2197
3729 771.7567 2312.2483
3757 779.7534 2336.2384
3761 782.4664 2344.3774
3762 1173.6921 2345.3696
3763 1173.6927 2345.3708
3764 782.7995 2345.3767
3777 787.4213 2359.2421
3784 788.1325 2361.3757
3787 789.0984 2364.2734
3791 790.7972 2369.3698
3798 793.7955 2378.3647
3816 1195.2111 2388.4076
3834 801.4587 2401.3543
3836 602.3292 2405.2877
3846 805.4620 2413.3642
3847 805.8022 2414.3848
3852 806.7505 2417.2297
3863 808.4564 2422.3474
3881 611.2964 2441.1565
3888 817.8206 2450.4400
3893 820.4296 2458.2670
3898 822.1169 2463.3289
3902 823.1368 2466.3886
3903 823.1392 2466.3958
3904 823.4343 2467.2811
3907 823.4475 2467.3207
3921 622.8315 2487.2969
3922 622.8608 2487.4141
3926 830.4752 2488.4038
3927 623.1085 2488.4049
3931 832.4354 2494.2844
3949 837.4735 2509.3987
3963 842.4548 2524.3426
3964 844.4292 2530.2658
3969 635.3613 2537.4161
3974 849.8069 2546.3989
3975 849.8355 2546.4847
3981 851.1465 2550.4177
3984 852.1177 2553.3313
3988 639.6116 2554.4173
3991 641.8571 2563.3993
3992 641.8580 2563.4029
3998 857.1497 2568.4273
3999 857.1514 2568.4324
4003 645.3322 2577.2997
4008 861.1169 2580.3289
4009 861.1464 2580.4174
4010 861.4533 2581.3381
4011 861.4731 2581.3975
4012 861.4769 2581.4089
4013 861.4858 2581.4356
4021 650.3494 2597.3685
4022 650.3505 2597.3729
4024 651.1203 2600.4521
4028 872.4421 2614.3045
4036 658.8712 2631.4557
4039 659.1162 2632.4357
4043 879.4170 2635.2292
4046 880.4667 2638.3783
4049 882.7986 2645.3740
4052 662.5950 2646.3509
4060 887.1813 2658.5221
4062 890.1603 2667.4591
4073 893.4893 2677.4461
4076 895.7542 2684.2408
4077 672.0679 2684.2425
4084 898.4237 2692.2493
4093 901.1213 2700.3421
4102 902.8206 2705.4400
4104 903.4103 2707.2091
4110 680.9012 2719.5757
4112 907.5384 2719.5934
4113 908.0809 2721.2209
4114 908.0857 2721.2353
4117 910.8576 2729.5510
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