MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 3786)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
345 629.0078 628.0005
358 634.2911 633.2838
385 327.2032 652.3918
498 702.7050 701.6977
499 703.0458 702.0385
518 715.7375 714.7302
537 724.0699 723.0626
577 745.9130 744.9057
588 751.8549 750.8476
622 765.3916 764.3843
625 765.9373 764.9300
661 783.4336 782.4263
663 783.9705 782.9632
685 793.9302 792.9229
710 801.9545 800.9472
715 807.9512 806.9439
721 813.8977 812.8904
740 827.1304 826.1231
766 420.7518 839.4890
803 852.5741 851.5668
882 887.2305 886.2232
979 927.9578 926.9505
1121 985.5267 984.5194
1271 1053.5206 1052.5133
1327 1080.0632 1079.0559
1388 557.3679 1112.7212
1474 382.9265 1145.7577
1488 578.5504 1155.0862
1559 591.3674 1180.7202
1599 600.0663 1198.1180
1689 615.6099 1229.2052
1690 615.6102 1229.2058
1720 620.1158 1238.2170
1756 627.6162 1253.2178
1758 628.1145 1254.2144
1786 635.6135 1269.2124
2049 463.9590 1388.8552
2108 706.4496 1410.8846
2137 713.1527 1424.2908
2233 738.9457 1475.8768
2235 369.9840 1475.9069
2257 498.2962 1491.8668
2300 755.3888 1508.7630
2386 775.4213 1548.8280
2446 787.4850 1572.9554
2526 805.4825 1608.9504
2527 805.5095 1609.0044
2563 809.4680 1616.9214
2589 544.6594 1630.9564
2592 408.9972 1631.9597
2610 820.9625 1639.9104
2629 550.9847 1649.9323
2633 827.9596 1653.9046
2681 419.0096 1672.0093
2694 841.4606 1680.9066
2768 861.2018 1720.3890
2801 867.5002 1732.9858
2806 870.0428 1738.0710
2808 581.3282 1740.9628
2829 587.6923 1760.0551
2831 441.2384 1760.9245
2929 905.0294 1808.0442
2943 607.2911 1818.8515
2979 924.5366 1847.0586
2980 925.0410 1848.0674
2988 927.5280 1853.0414
2998 929.0015 1855.9884
3001 620.3223 1857.9451
3091 959.9908 1917.9670
3109 644.6988 1931.0746
3110 966.5450 1931.0754
3148 656.3796 1966.1170
3161 989.5322 1977.0498
3165 990.5439 1979.0732
3174 661.7203 1982.1391
3175 992.5803 1983.1460
3176 992.8099 1983.6052
3203 1002.5393 2003.0640
3204 669.7308 2006.1706
3207 670.0612 2007.1618
3208 670.0680 2007.1822
3210 1005.5571 2009.0996
3232 674.0547 2019.1423
3242 1013.0384 2024.0622
3246 677.3656 2029.0750
3247 1016.5280 2031.0414
3248 678.3925 2032.1557
3259 680.3444 2038.0114
3262 1020.4983 2038.9820
3288 686.3882 2056.1428
3340 699.3748 2095.1026
3399 532.5060 2125.9949
3412 427.0555 2130.2411
3429 713.7178 2138.1316
3452 538.7936 2151.1453
3457 718.4170 2152.2292
3459 718.7521 2153.2345
3478 1085.5966 2169.1786
3493 1088.6189 2175.2232
3497 727.0820 2178.2242
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