MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 3786)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
111 401.7256 400.7183
112 402.2466 401.2393
113 402.2472 401.2399
114 402.2480 401.2407
115 403.2315 402.2242
118 404.2518 403.2445
119 407.2277 406.2204
120 407.2279 406.2206
121 408.2363 407.2290
122 409.2432 408.2359
123 409.2435 408.2362
124 409.2438 408.2365
125 412.6995 411.6922
126 413.7435 412.7362
127 413.7575 412.7502
128 414.2386 413.2313
129 414.2420 413.2347
130 416.2628 415.2555
131 416.2628 415.2555
132 416.2630 415.2557
133 416.2640 415.2567
134 416.2879 415.2806
135 416.2880 415.2807
136 416.2882 415.2809
137 416.2886 415.2813
138 416.2893 415.2820
145 418.2671 417.2598
146 421.2567 420.2494
147 421.2578 420.2505
148 423.2946 422.2873
149 425.2388 424.2315
150 426.2182 425.2109
151 426.5646 425.5573
152 428.2888 427.2815
153 430.2794 429.2721
154 430.3036 429.2963
155 430.3036 429.2963
156 430.3037 429.2964
157 430.3037 429.2964
158 430.3048 429.2975
159 430.8570 429.8497
160 432.2578 431.2505
161 432.2589 431.2516
162 432.2607 431.2534
163 437.2169 436.2096
164 439.7421 438.7348
165 440.2520 439.2447
166 440.7312 439.7239
167 444.2940 443.2867
168 444.2944 443.2871
169 444.2955 443.2882
170 444.3198 443.3125
171 446.2731 445.2658
172 446.2747 445.2674
173 446.2752 445.2679
174 448.2570 447.2497
175 450.2480 449.2407
176 450.7828 449.7755
177 451.2675 450.2602
178 451.2678 450.2605
179 451.2695 450.2622
181 462.2367 461.2294
182 467.7740 466.7667
183 468.2753 467.2680
184 469.2654 468.2581
185 472.3258 471.3185
193 475.9577 474.9504
194 476.2107 475.2034
195 484.3291 483.3218
197 486.2830 485.2757
209 501.7837 500.7764
214 506.3099 505.3026
215 506.3105 505.3032
216 510.2367 509.2294
217 510.2383 509.2310
218 514.8022 513.7949
225 516.3143 515.3070
230 522.2706 521.2633
231 524.2369 523.2296
232 528.2789 527.2716
241 533.2809 532.2736
242 544.2929 543.2856
245 546.8087 545.8014
246 552.8257 551.8184
247 555.1049 554.0976
248 557.0807 556.0734
256 561.8336 560.8263
259 571.0671 570.0598
260 571.0720 570.0647
265 578.2987 577.2914
267 581.8287 580.8214
277 588.8199 587.8126
278 591.7986 590.7913
279 599.6757 598.6684
280 600.0104 599.0031
288 601.9919 600.9846
289 601.9932 600.9859
307 607.3278 606.3205
335 622.8207 621.8134
338 312.1856 622.3566
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