MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 3786)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
850 872.4510 871.4437 871.4399 4.36 1 1 0.91 +1Score > 40 indicates identity
Score > 13 indicates homology
AITGEEPR
3770 784.0576 2349.1510 2349.1311 8.48 1 1 0.99 +1Score > 39 indicates identity
Score > 13 indicates homology
NNFIMQFQADLLNLGVERPE + 2 Deamidated (NQ)
4341 1004.1892 3009.5458 3009.5198 8.62 1 1 0.91 +1Score > 35 indicates identity
Score > 13 indicates homology
HQILIGNMMSSRSPVDSLILMHGVGTGK + 2 Oxidation (M)
4420 785.1490 3136.5669 3136.5838 -5.38 1 1 1 +1Score > 36 indicates identity
Score > 13 indicates homology
NMAVGITSMASRVGSIIAPYFVYLGAYNR + Oxidation (M)
2644 833.4940 1664.9734 1664.9573 9.69 1 1 1 +1Score > 31 indicates identity
Score > 13 indicates homology
VNRPAQLARVLSVLE + Deamidated (NQ)
4304 985.1923 2952.5551 2952.5738 -6.34 1 1 1 +1Score > 35 indicates identity
Score > 13 indicates homology
QSITLIEMMVVITLIGIIGGALAFNMR + Deamidated (NQ); 3 Oxidation (M)
4358 1010.5640 3028.6702 3028.7035 -11.0 1 1 1 +1Score > 31 indicates identity
Score > 13 indicates homology
LALVVIFLLLASLFQPLTAQSSCPGNKK + Deamidated (NQ)
3623 1122.1150 2242.2154 2242.1746 18.2 1 1 0.9 +1Score > 35 indicates identity
Score > 13 indicates homology
NFVFSTKAINTLFAAQQTNK
4252 963.4938 2887.4596 2887.5075 -16.6 1 1 1 +1Score > 37 indicates identity
Score > 13 indicates homology
LGATPIVMQLPIGAEADFTGVVDLVSMK + Oxidation (M)
3065 633.6635 1897.9687 1897.9897 -11.1 1 1 1 +1Score > 39 indicates identity
Score > 13 indicates homology
WSIIAAQLPGRTDNDIK + Deamidated (NQ)
3307 692.7198 2075.1376 2075.1164 10.2 1 1 0.9 +1Score > 35 indicates identity
Score > 13 indicates homology
LNSAVFPGAQGGPLEHVIAAK
4303 984.5305 2950.5697 2950.5988 -9.88 1 1 1 +1Score > 34 indicates identity
Score > 13 indicates homology
HLDRIDSVLLTHIGADNLPGINGLLQR + Deamidated (NQ)
4649 908.7544 3630.9885 3631.0477 -16.3 0 1 1 +1Score > 28 indicates identity
Score > 13 indicates homology
VQLLGHIFGDIYPSIFHVLALNIAVVGVGVIMAR
4347 756.0956 3020.3533 3020.3081 15.0 1 1 1 +1Score > 35 indicates identity
Score > 13 indicates homology
SDVITNVIDANADTGLDDDPWMKDGTPE + Deamidated (NQ); Oxidation (M)
2944 910.4454 1818.8762 1818.8683 4.38 1 0 1 +1Score > 40 indicates identity
Score > 13 indicates homology
RDAVIGHGTSFLMQDR + Deamidated (NQ); Oxidation (M)
4244 960.1463 2877.4171 2877.4331 -5.56 1 0 0.98 +1Score > 37 indicates identity
Score > 13 indicates homology
KPDPEPPTPKPPATMTIPASRADQYR + Deamidated (NQ); Oxidation (M)
567 736.3680 735.3607 735.3473 18.3 0 0 0.95 +1Score > 42 indicates identity
Score > 13 indicates homology
MDLNVK + Deamidated (NQ); Oxidation (M)
4628 893.4486 3569.7653 3569.8051 -11.1 1 0 1 +1Score > 35 indicates identity
Score > 13 indicates homology
CNGLISHITIDKGSFLADNFYIPMVLIPYSR + Oxidation (M)
654 777.4011 776.3938 776.3963 -3.17 1 0 1 +1Score > 42 indicates identity
Score > 13 indicates homology
KAMAGQR + Oxidation (M)
4505 1080.5885 3238.7437 3238.7020 12.9 1 0 1 +1Score > 32 indicates identity
Score > 13 indicates homology
GAIWALGLLVSAVAMLGGEGQSQAIADAAAALAR + Deamidated (NQ); Oxidation (M)
4186 703.1396 2808.5293 2808.5745 -16.1 1 0 1 +1Score > 32 indicates identity
Score > 13 indicates homology
YQLLTSVLQCLTKILTIDMVITVK + Oxidation (M)
4372 764.3998 3053.5701 3053.5777 -2.48 1 0 0.96 +1Score > 35 indicates identity
Score > 13 indicates homology
TCIVIAHRLSTIQNADIIAVMAQGVVIE + 3 Deamidated (NQ); Oxidation (M)
4830 885.2776 5305.6219 5305.7011 -14.9 0 0 1 +1Score > 29 indicates identity
Score > 13 indicates homology
IGITYFIMQYINAAGVALGYQDNVWLLHLLPFNSMGNIIVVAFIVTR + 6 Deamidated (NQ); 2 Oxidation (M)
2699 843.9440 1685.8734 1685.8989 -15.1 0 0 0.93 +1Score > 40 indicates identity
Score > 13 indicates homology
FGTQPAVVALLGDVDGK
3118 969.0463 1936.0780 1936.0993 -11.0 1 0 1 +1Score > 34 indicates identity
Score > 13 indicates homology
AVESILGAVTVPVQLGGGIR + Deamidated (NQ)
4209 711.3434 2841.3445 2841.3127 11.2 0 0 1 +1Score > 37 indicates identity
Score > 13 indicates homology
HHTIQNSDDAYVQLDNLPGMSLVAGK + 3 Deamidated (NQ); Oxidation (M)
3403 1064.5531 2127.0916 2127.1180 -12.4 0 0 1 +1Score > 39 indicates identity
Score > 13 indicates homology
AAGVINNVVASVPMVVNAVMR + Deamidated (NQ); Oxidation (M)
4157 927.4831 2779.4275 2779.4532 -9.24 0 0 1 +1Score > 36 indicates identity
Score > 13 indicates homology
SFPIAVALGADPATILDAVTPVPDTLSE
4473 641.5643 3202.7851 3202.8329 -14.9 1 0 1 +1Score > 29 indicates identity
Score > 13 indicates homology
LDAVLASGDAIRNHPLYGTVLVLVLLGALTK + Deamidated (NQ)
3659 756.3892 2266.1458 2266.1780 -14.2 1 0 0.94 +1Score > 39 indicates identity
Score > 13 indicates homology
VQLTQQFCKLFQALGLNTR + 2 Deamidated (NQ)
4321 744.8832 2975.5037 2975.5136 -3.34 0 0 0.99 +1Score > 36 indicates identity
Score > 13 indicates homology
MGISPSLFSTQPLWQIAMVLQATQAQK + 2 Deamidated (NQ)
4620 886.9473 3543.7601 3543.6936 18.8 1 0 1 +1Score > 35 indicates identity
Score > 13 indicates homology
AVQTGIGQLDGINVAIAVMDFQFMGGSMGSVVGEK + 2 Deamidated (NQ); Oxidation (M)
4753 814.8061 4068.9941 4068.9531 10.1 1 0 1 +1Score > 33 indicates identity
Score > 13 indicates homology
CGMIYMEPQMLGWRPLMVSWINTLPQSVSIIQKE + 2 Deamidated (NQ); 2 Oxidation (M)
4685 538.0037 3758.9750 3758.9196 14.7 0 0 1 +1Score > 32 indicates identity
Score > 13 indicates homology
DNVIIDNLCQIAHNVHIGTGTAVAGGVIMAGSLTVGR + Oxidation (M)
4789 1115.5758 4458.2741 4458.2127 13.8 1 0 1 +1Score > 31 indicates identity
Score > 13 indicates homology
HSAATDIASSLSDDQVPQAFLFMLLVQFGTMVIDRALYLR + 3 Deamidated (NQ); Oxidation (M)
4561 1112.5940 3334.7602 3334.7382 6.59 1 0 1 +1Score > 33 indicates identity
Score > 13 indicates homology
NLIAAHSHIRGLGVDVDSLQPRPASQGLVGQE + Deamidated (NQ)
4587 1131.2633 3390.7681 3390.7055 18.4 1 0 1 +1Score > 34 indicates identity
Score > 13 indicates homology
SGGQLAAVTQWIAAATVSRAEPPQSQPSVEPVR + 3 Deamidated (NQ)
2169 722.4402 1442.8658 1442.8609 3.44 1 0 0.93 +1Score > 30 indicates identity
Score > 13 indicates homology
KLNALITAAPYIR
4841 917.9344 5501.5627 5501.5784 -2.85 1 0 1 +1Score > 28 indicates identity
Score > 13 indicates homology
ANGQIISANGHAIGSRIIGQPFTGPAYFHSRPSAAGNGYDASNSGGTNLGPTNQK + 6 Deamidated (NQ)
4518 815.4374 3257.7205 3257.6754 13.8 1 0 1 +1Score > 33 indicates identity
Score > 13 indicates homology
LICIDQDQQAIDFANNLFKDNPNVIVIK
4668 463.7499 3701.9410 3701.8897 13.8 0 0 1 +1Score > 32 indicates identity
Score > 13 indicates homology
MNLMLALTVNTLLTALLTIIMFWLPQLNSYAE + 3 Deamidated (NQ); 3 Oxidation (M)
2140 714.4262 1426.8378 1426.8409 -2.12 0 0 1 +1Score > 34 indicates identity
Score > 13 indicates homology
HQLPGVLAGLAPVR
3934 834.4080 2500.2022 2500.2016 0.22 0 0 1 +1Score > 38 indicates identity
Score > 13 indicates homology
GGCLNLSQSNVFLDLVQGANHQK + 2 Deamidated (NQ)
4311 988.7849 2963.3329 2963.3503 -5.88 1 0 1 +1Score > 35 indicates identity
Score > 13 indicates homology
MPSLLANMAEGMLLPLPSVQWNHNHE + 3 Deamidated (NQ); 2 Oxidation (M)
4431 1053.2223 3156.6451 3156.5835 19.5 0 0 1 +1Score > 35 indicates identity
Score > 13 indicates homology
SINALLMTITGFFIGGPSNMVSSAISADLGR + Deamidated (NQ); Oxidation (M)
4457 1061.5240 3181.5502 3181.5682 -5.65 1 0 0.99 +1Score > 36 indicates identity
Score > 13 indicates homology
ELIIWSNPAFGFVQLPDAYATGSSIMPQK + 2 Deamidated (NQ)
4600 858.4338 3429.7061 3429.7380 -9.30 1 0 0.98 +1Score > 35 indicates identity
Score > 13 indicates homology
AMCQVAALDDVDTVMAAIVGAAGLLPTMAAVKAGK + Deamidated (NQ)
2234 492.9664 1475.8774 1475.8752 1.50 0 0 1 +1Score > 29 indicates identity
Score > 13 indicates homology
IFIFQLLNILNK + Deamidated (NQ)
4222 955.4677 2863.3813 2863.4060 -8.64 1 0 1 +1Score > 37 indicates identity
Score > 13 indicates homology
AINVNGVSFNNVDTSSLGAAQVRQSASR + 2 Deamidated (NQ)
4164 697.8661 2787.4353 2787.4079 9.85 1 0 1 +1Score > 36 indicates identity
Score > 13 indicates homology
VALGNITNVAAAANNAKFNSATWAAPVK + 4 Deamidated (NQ)
4361 607.3130 3031.5286 3031.5318 -1.04 1 0 1 +1Score > 36 indicates identity
Score > 13 indicates homology
ATSNICSNQALLALAASIAMTALGKQGVQE + Deamidated (NQ)
180 462.2282 461.2209 461.2122 19.0 0 0 1 +1Score > 47 indicates identity
Score > 13 indicates homology
ATAAE
4170 931.5205 2791.5397 2791.5769 -13.3 0 0 1 +1Score > 32 indicates identity
Score > 13 indicates homology
LAVSGTVNDLAVMGAKPLALSLSLIIPE
4699 766.3809 3826.8681 3826.8842 -4.21 1 0 1 +1Score > 34 indicates identity
Score > 13 indicates homology
DAVARAQGMAAAPAAQAGAAPQPTQSPMAIGAALHAAATAR + 2 Deamidated (NQ); Oxidation (M)
4654 731.3831 3651.8791 3651.8930 -3.81 0 0 1 +1Score > 33 indicates identity
Score > 13 indicates homology
MPDPLALAISTGEPAGIGPDITIGALLQLAGQNGHAR + Deamidated (NQ); Oxidation (M)
4026 870.4655 2608.3747 2608.3571 6.75 1 0 1 +1Score > 36 indicates identity
Score > 13 indicates homology
VLILMVVDNKWTDHIDALDQLR + 2 Deamidated (NQ)
4091 675.3449 2697.3505 2697.4014 -18.9 1 0 0.96 +1Score > 38 indicates identity
Score > 13 indicates homology
TSNPGSGFLQDLLVEGKPLYLHLAE
4218 713.8954 2851.5525 2851.5054 16.5 1 0 1 +1Score > 32 indicates identity
Score > 13 indicates homology
EPYAKAINSQIFPGIQGGPLMHVIAGK + Oxidation (M)
4253 963.4959 2887.4659 2887.4150 17.6 1 0 1 +1Score > 37 indicates identity
Score > 13 indicates homology
VLFLGMNPGPFGMAQTGVPFGEVNHVR + Deamidated (NQ); Oxidation (M)
1588 597.3173 1192.6200 1192.6088 9.46 0 0 1 +1Score > 41 indicates identity
Score > 13 indicates homology
NPVEPLAAPQR + 2 Deamidated (NQ)
3878 814.3924 2440.1554 2440.1845 -11.9 0 0 1 +1Score > 38 indicates identity
Score > 13 indicates homology
QLGLQPWAPVSLAMHQFTDQR + 2 Deamidated (NQ); Oxidation (M)
4758 1022.5541 4086.1873 4086.1071 19.6 1 0 1 +1Score > 29 indicates identity
Score > 13 indicates homology
LQALIYPVLQALDFNTPSYQQSMNTPILPRHVMVR + Deamidated (NQ); 2 Oxidation (M)
4032 873.7426 2618.2060 2618.2244 -7.02 1 0 1 +1Score > 37 indicates identity
Score > 13 indicates homology
LYTSMYDQQIAQQMTAGKGLGLAE + 2 Deamidated (NQ)
4165 697.8782 2787.4837 2787.4914 -2.77 1 0 1 +1Score > 34 indicates identity
Score > 13 indicates homology
VATMIALMKVIDNPYQDIPLAAVLR + Deamidated (NQ); 2 Oxidation (M)
4485 1070.5614 3208.6624 3208.6325 9.30 1 0 1 +1Score > 34 indicates identity
Score > 13 indicates homology
MDLGKIGNPNTLILLNQGSNYALTYQIDK + 2 Deamidated (NQ)
4536 826.9525 3303.7809 3303.8257 -13.6 0 0 1 +1Score > 32 indicates identity
Score > 13 indicates homology
NLLGNPQASIAQLIVTVFLGLVIGAIFYDLK + 4 Deamidated (NQ)
3432 715.3684 2143.0834 2143.1096 -12.2 1 0 1 +1Score > 39 indicates identity
Score > 13 indicates homology
LKFVGTATAGMDHVDQALLR + Deamidated (NQ)
3775 590.3239 2357.2665 2357.2460 8.68 1 0 1 +1Score > 35 indicates identity
Score > 13 indicates homology
GLMVGQGINMAHLRGTLTAFVR + Oxidation (M)
4592 681.9845 3404.8861 3404.9084 -6.56 1 0 1 +1Score > 28 indicates identity
Score > 13 indicates homology
AWLLFAHQGAEPGHKTLLAALQAEPLLALGLR
4623 1185.6108 3553.8106 3553.8133 -0.78 1 0 1 +1Score > 34 indicates identity
Score > 13 indicates homology
RPGGAANVAMNIASLGGHAHIVGLTGMDEPAKVLTE
4464 799.3989 3193.5665 3193.6044 -11.9 1 0 1 +1Score > 36 indicates identity
Score > 13 indicates homology
VSNVTIWGNHSTTQVPDFVNAQIGGVPVKE + Deamidated (NQ)
3363 704.3748 2110.1026 2110.0735 13.8 1 0 0.98 +1Score > 38 indicates identity
Score > 13 indicates homology
RVIDGITNGVWITSFFVGE + Deamidated (NQ)
3685 571.3243 2281.2681 2281.2641 1.75 1 0 1 +1Score > 33 indicates identity
Score > 13 indicates homology
NKILVATVDNASILLQIDNAR + Deamidated (NQ)
4449 795.4407 3177.7337 3177.7842 -15.9 1 0 1 +1Score > 31 indicates identity
Score > 13 indicates homology
SGQAPLAFSYLAFSVLGGLLATWAGLILTKL
4502 1079.5688 3235.6846 3235.7005 -4.92 1 0 1 +1Score > 34 indicates identity
Score > 13 indicates homology
ARLYWLQVDNQLPGTMFPVVFHPVAPPK + Oxidation (M)
4713 977.7830 3907.1029 3907.1534 -12.9 1 0 1 +1Score > 30 indicates identity
Score > 13 indicates homology
HPFIIIGQTASMLYFLIILVLMPITSLTENYLLK + 2 Deamidated (NQ)
4487 803.4161 3209.6353 3209.6092 8.14 1 0 1 +1Score > 35 indicates identity
Score > 13 indicates homology
LLNSDIDLVDQSILNVQIDIVENQAWQN + Deamidated (NQ)
4375 765.1683 3056.6441 3056.6508 -2.19 1 0 1 +1Score > 32 indicates identity
Score > 13 indicates homology
ESALVMNNVLLAVAALVVFTGTVWPLIAE + Deamidated (NQ); Oxidation (M)
4806 770.7278 4618.3231 4618.3473 -5.22 1 0 1 +1Score > 31 indicates identity
Score > 13 indicates homology
IMGHALTLPAAIWTRLNIAWIIFFLFCGAANLYVAFTYQE + 3 Deamidated (NQ)
2991 619.2859 1854.8359 1854.8557 -10.7 1 0 1 +1Score > 39 indicates identity
Score > 13 indicates homology
NQMLENSFLVDTVTNK + 3 Deamidated (NQ)
3810 795.7178 2384.1316 2384.1583 -11.2 1 0 1 +1Score > 39 indicates identity
Score > 13 indicates homology
TMVVSYRVPHNAAVQVYDYR + Deamidated (NQ); Oxidation (M)
4109 907.4800 2719.4182 2719.3713 17.2 1 0 1 +1Score > 36 indicates identity
Score > 13 indicates homology
GLVPAIVQDYLDGTVLMLAWMNRE + Oxidation (M)
4346 1007.7907 3020.3503 3020.3783 -9.29 1 0 1 +1Score > 34 indicates identity
Score > 13 indicates homology
VDFANLTKFQNIYALMQCTPDISSDE + Deamidated (NQ)
4596 854.4577 3413.8017 3413.7453 16.5 1 0 1 +1Score > 32 indicates identity
Score > 13 indicates homology
VTALVGPNGSGKSTVAALLQNLYQPTGGQVLLDE + 4 Deamidated (NQ)
3726 771.0581 2310.1525 2310.1539 -0.61 1 0 1 +1Score > 39 indicates identity
Score > 13 indicates homology
LQSVTIQDALNHPNWSMGRK + Oxidation (M)
4063 890.1617 2667.4633 2667.4483 5.61 1 0 1 +1Score > 32 indicates identity
Score > 13 indicates homology
APGPSVIAAPPSGGGNLLQTLVLPPSKE + Deamidated (NQ)
3066 634.0169 1899.0289 1898.9989 15.8 0 0 1 +1Score > 37 indicates identity
Score > 13 indicates homology
FDNLIAITNGIDLITHK + 2 Deamidated (NQ)
4267 967.8464 2900.5174 2900.4919 8.77 1 0 1 +1Score > 35 indicates identity
Score > 13 indicates homology
AGADYLVLGRPIYAADDPKAAAQAIIQE + Deamidated (NQ)
4586 1130.9263 3389.7571 3389.7215 10.5 1 0 1 +1Score > 34 indicates identity
Score > 13 indicates homology
SGGQLAAVTQWIAAATVSRAEPPQSQPSVEPVR + 2 Deamidated (NQ)
4826 1050.7240 5248.5836 5248.4869 18.4 1 0 1 +1Score > 30 indicates identity
Score > 13 indicates homology
WPQQLSHQEMLAGHSQQILNNNKPAGNQSKPPIVMASNNVFSIGPYR + 7 Deamidated (NQ); Oxidation (M)
1 303.0617 302.0544
2 303.1784 302.1711
3 303.1784 302.1711
4 303.1790 302.1717
5 304.1624 303.1551
6 304.1628 303.1555
7 305.0105 304.0032
8 305.0112 304.0039
9 309.0327 308.0254
10 313.7039 312.6966
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