MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 201–300 (out of 3786)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
751 416.2471 830.4796 830.4749 5.70 1 25 0.95 +1Score > 40 indicates identity
Score > 37 indicates homology		 TLEQIVK + Deamidated (NQ)
292 602.3532 601.3459 601.3435 4.00 0 25 0.87 +1Score > 45 indicates identity
Score > 37 indicates homology		 ALDGVK
1383 371.5471 1111.6195 1111.6349 -13.9 0 25 0.29 +1Score > 38 indicates identity
Score > 32 indicates homology		 QLGANAVIGIR + Deamidated (NQ)
435 338.1875 674.3604 674.3711 -15.9 0 25 0.92 +1Score > 43 indicates identity
Score > 37 indicates homology		 VGSSAVR
621 382.7335 763.4524 763.4480 5.89 0 25 0.54 +1Score > 35 indicates identity IINYIK + Deamidated (NQ)
933 456.7318 911.4490 911.4461 3.24 1 25 0.11 +1Score > 40 indicates identity
Score > 28 indicates homology		 ESVIHDGR
619 382.7204 763.4262 763.4228 4.51 1 25 0.14 +1Score > 41 indicates identity
Score > 29 indicates homology		 IIAEYR
421 336.7119 671.4092 671.3966 18.8 1 25 0.8 +1Score > 44 indicates identity
Score > 36 indicates homology		 GVVEIR
836 433.7184 865.4222 865.4181 4.75 1 25 0.21 +1Score > 41 indicates identity
Score > 31 indicates homology		 AIESDGFK
254 561.3022 560.2949 560.2918 5.53 0 25 0.39 +1Score > 44 indicates identity
Score > 33 indicates homology		 DATVR
768 421.2641 840.5136 840.5069 8.05 1 25 0.69 +1Score > 36 indicates identity QLKDIPK
604 760.4321 759.4248 759.4239 1.25 0 25 0.87 +1Score > 42 indicates identity
Score > 37 indicates homology		 LNSALSR
1558 1181.7252 1180.7179 1180.7067 9.48 1 25 0.27 +1Score > 32 indicates identity DVVPVLIEGLK
1643 304.6741 1214.6673 1214.6619 4.47 1 25 0.23 +1Score > 40 indicates identity
Score > 31 indicates homology		 ALIQSAIGSAER
600 380.2358 758.4570 758.4538 4.29 0 25 0.62 +1Score > 40 indicates identity
Score > 35 indicates homology		 LTVADIK
1695 616.8282 1231.6418 1231.6237 14.7 1 25 0.93 +1Score > 41 indicates identity
Score > 37 indicates homology		 ALNELFPDWK
326 308.6872 615.3598 615.3704 -17.1 0 25 0.32 +1Score > 46 indicates identity
Score > 32 indicates homology		 AIVSAR
1737 415.8841 1244.6305 1244.6150 12.5 0 25 0.88 +1Score > 42 indicates identity
Score > 37 indicates homology		 WQDIGGLDSVR
1011 471.7925 941.5704 941.5546 16.9 1 25 0.37 +1Score > 38 indicates identity
Score > 33 indicates homology		 ILVKNQVE
1626 606.3451 1210.6756 1210.6822 -5.42 1 25 0.61 +1Score > 38 indicates identity
Score > 35 indicates homology		 QSILLRYYR
2284 501.9196 1502.7370 1502.7148 14.8 0 25 0.37 +1Score > 41 indicates identity
Score > 33 indicates homology		 MTQVGQTHTSGSIR + Deamidated (NQ)
1058 319.8685 956.5837 956.5728 11.3 1 25 0.72 +1Score > 39 indicates identity
Score > 36 indicates homology		 KMLPNLLK + Deamidated (NQ)
2747 571.3331 1710.9775 1710.9767 0.42 1 25 0.46 +1Score > 34 indicates identity QQPITTTLELVDIIK
936 457.7914 913.5682 913.5596 9.43 0 25 0.32 +1Score > 36 indicates identity
Score > 32 indicates homology		 ALLSQLLR + Deamidated (NQ)
589 377.2173 752.4200 752.4181 2.62 0 25 0.13 +1Score > 38 indicates identity
Score > 28 indicates homology		 ALDVHAK
1096 486.7663 971.5180 971.5148 3.31 0 25 0.27 +1Score > 42 indicates identity
Score > 31 indicates homology		 SAGLQVQNR
187 473.3093 472.3020 472.3009 2.31 0 25 0.66 +1Score > 40 indicates identity
Score > 35 indicates homology		 IGGVK
1018 473.7556 945.4966 945.5131 -17.4 1 25 0.8 +1Score > 42 indicates identity
Score > 36 indicates homology		 TNVGEISVK
1287 529.8044 1057.5942 1057.6131 -17.9 1 24 0.39 +1Score > 41 indicates identity
Score > 33 indicates homology		 QTKAIAAQVK + Deamidated (NQ)
1568 593.3316 1184.6486 1184.6401 7.25 1 24 0.19 +1Score > 40 indicates identity
Score > 30 indicates homology		 IQELINAVQR + 2 Deamidated (NQ)
432 338.1815 674.3484 674.3599 -17.0 0 24 0.64 +1Score > 43 indicates identity
Score > 35 indicates homology		 DGVSAVK
1159 500.2851 998.5556 998.5621 -6.46 1 24 0.71 +1Score > 39 indicates identity
Score > 35 indicates homology		 QDALAARVR
646 388.6934 775.3722 775.3864 -18.3 0 24 0.81 +1Score > 43 indicates identity
Score > 36 indicates homology		 WNSALGK + Deamidated (NQ)
1674 613.3342 1224.6538 1224.6462 6.22 1 24 0.17 +1Score > 39 indicates identity
Score > 29 indicates homology		 QVAELINADPR
1012 472.2471 942.4796 942.4667 13.8 1 24 0.72 +1Score > 39 indicates identity
Score > 35 indicates homology		 KVMQMYK + Oxidation (M)
1740 625.3269 1248.6392 1248.6350 3.39 1 24 0.36 +1Score > 41 indicates identity
Score > 32 indicates homology		 ATFLQVRVQGE + 2 Deamidated (NQ)
1745 625.8328 1249.6510 1249.6740 -18.4 1 24 0.2 +1Score > 41 indicates identity
Score > 30 indicates homology		 VNELIYLVMR + Deamidated (NQ)
323 616.3431 615.3358 615.3340 2.94 0 24 0.14 +1Score > 44 indicates identity
Score > 28 indicates homology		 VSPSAR
1423 565.7770 1129.5394 1129.5516 -10.8 1 24 0.15 +1Score > 41 indicates identity
Score > 29 indicates homology		 SPQWVRDNK + Deamidated (NQ)
1742 625.8319 1249.6492 1249.6740 -19.8 1 24 0.32 +1Score > 41 indicates identity
Score > 32 indicates homology		 VNELIYLVMR + Deamidated (NQ)
328 308.6876 615.3606 615.3704 -15.9 1 24 0.23 +1Score > 45 indicates identity
Score > 30 indicates homology		 AGGGKVK
2811 872.4982 1742.9818 1742.9930 -6.42 1 24 0.093 +1Score > 35 indicates identity
Score > 26 indicates homology		 QLTKVLLFQNGKPVR + 3 Deamidated (NQ)
1513 581.8033 1161.5920 1161.5812 9.35 0 24 0.3 +1Score > 42 indicates identity
Score > 31 indicates homology		 QTAAMVAAQQK + Oxidation (M)
422 336.7130 671.4114 671.4078 5.38 1 24 0.26 +1Score > 42 indicates identity
Score > 31 indicates homology		 RVQIR + Deamidated (NQ)
845 435.2467 868.4788 868.4807 -2.11 0 24 0.32 +1Score > 37 indicates identity
Score > 32 indicates homology		 ILTYGFR
596 380.2218 758.4290 758.4286 0.57 0 24 0.66 +1Score > 44 indicates identity
Score > 35 indicates homology		 INASNLK
1234 345.5028 1033.4866 1033.4862 0.32 1 24 0.17 +1Score > 41 indicates identity
Score > 29 indicates homology		 EMDAGVSGLR
615 762.3919 761.3846 761.3742 13.7 1 24 0.58 +1Score > 42 indicates identity
Score > 34 indicates homology		 DLMEVR
1351 547.7853 1093.5560 1093.5516 4.06 0 24 0.93 +1Score > 41 indicates identity
Score > 36 indicates homology		 AATNGAPGVAHK + Deamidated (NQ)
2077 701.3991 1400.7836 1400.7598 17.0 1 24 0.29 +1Score > 38 indicates identity
Score > 31 indicates homology		 HPRLLYMGTLGK + Oxidation (M)
759 419.2492 836.4838 836.4830 1.05 0 24 0.25 +1Score > 36 indicates identity
Score > 30 indicates homology		 LIAVYMK
303 604.3683 603.3610 603.3592 3.09 0 24 0.4 +1Score > 40 indicates identity
Score > 32 indicates homology		 ISSGLK
953 460.7480 919.4814 919.4797 1.91 0 24 0.28 +1Score > 42 indicates identity
Score > 31 indicates homology		 IGNMVLTR + Deamidated (NQ); Oxidation (M)
1344 544.3111 1086.6076 1086.6073 0.29 0 24 0.18 +1Score > 40 indicates identity
Score > 29 indicates homology		 VPIQIQNFK + Deamidated (NQ)
2115 472.5760 1414.7062 1414.7052 0.69 0 24 0.51 +1Score > 41 indicates identity
Score > 33 indicates homology		 TANAIVSQQTPQR + 2 Deamidated (NQ)
324 616.3456 615.3383 615.3340 7.00 0 24 0.25 +1Score > 44 indicates identity
Score > 30 indicates homology		 VSPSAR
460 689.3930 688.3857 688.3755 14.8 1 24 0.95 +1Score > 43 indicates identity
Score > 36 indicates homology		 DDKAIK
2679 836.9675 1671.9204 1671.9294 -5.38 1 24 0.065 +1Score > 37 indicates identity
Score > 24 indicates homology		 LKVSVLNTIDTILNE + Deamidated (NQ)
1985 679.8668 1357.7190 1357.6990 14.8 1 24 0.29 +1Score > 40 indicates identity
Score > 31 indicates homology		 NVITWQELQAR + Deamidated (NQ)
2074 701.3830 1400.7514 1400.7623 -7.77 1 24 0.11 +1Score > 40 indicates identity
Score > 27 indicates homology		 SVTQVNRDLLAGK + Deamidated (NQ)
2462 790.4507 1578.8868 1578.8729 8.81 1 24 0.46 +1Score > 35 indicates identity
Score > 33 indicates homology		 AAVQEGIVPGGGIALAR + Deamidated (NQ)
898 447.7387 893.4628 893.4607 2.45 1 24 0.31 +1Score > 41 indicates identity
Score > 31 indicates homology		 LSGELYGR
1681 614.8383 1227.6620 1227.6459 13.2 1 24 0.96 +1Score > 40 indicates identity
Score > 36 indicates homology		 QAQQLLELQR + 2 Deamidated (NQ)
1627 606.3457 1210.6768 1210.6822 -4.42 1 24 0.46 +1Score > 38 indicates identity
Score > 33 indicates homology		 QSILLRYYR
3250 1017.5400 2033.0654 2033.0252 19.8 1 24 0.19 +1Score > 38 indicates identity
Score > 29 indicates homology		 NQVSFLLNTGVMQPSEIR + Deamidated (NQ)
726 407.7578 813.5010 813.4960 6.20 1 24 0.28 +1Score > 39 indicates identity
Score > 30 indicates homology		 LGAEVVVK
1286 529.7979 1057.5812 1057.5768 4.22 1 24 0.52 +1Score > 41 indicates identity
Score > 33 indicates homology		 VVITADEGVR
1611 601.3418 1200.6690 1200.6503 15.7 1 24 0.4 +1Score > 40 indicates identity
Score > 32 indicates homology		 SWALVDILER
597 380.2346 758.4546 758.4538 1.12 0 23 0.6 +1Score > 41 indicates identity
Score > 34 indicates homology		 TIVADLK
2035 462.9206 1385.7400 1385.7337 4.56 0 23 0.21 +1Score > 40 indicates identity
Score > 29 indicates homology		 QITAAMQQLLPR + Deamidated (NQ); Oxidation (M)
484 351.6934 701.3722 701.3821 -14.0 0 23 0.63 +1Score > 45 indicates identity
Score > 34 indicates homology		 GGGGSLVR
752 416.2473 830.4800 830.4749 6.18 1 23 0.91 +1Score > 40 indicates identity
Score > 36 indicates homology		 TQVELLK + Deamidated (NQ)
2471 791.3882 1580.7618 1580.7769 -9.55 1 23 0.34 +1Score > 41 indicates identity
Score > 31 indicates homology		 QDIQKLFSTWCR
224 516.3046 515.2973 515.2955 3.57 0 23 0.46 +1Score > 47 indicates identity
Score > 33 indicates homology		 ALAQL + Deamidated (NQ)
2127 474.5819 1420.7239 1420.7166 5.09 0 23 0.13 +1Score > 41 indicates identity
Score > 27 indicates homology		 LGLSNMQGMSVLR + Oxidation (M)
1023 474.2391 946.4636 946.4542 9.99 1 23 0.96 +1Score > 42 indicates identity
Score > 36 indicates homology		 EAVQCAIR + Deamidated (NQ)
858 437.7552 873.4958 873.4807 17.3 1 23 0.52 +1Score > 41 indicates identity
Score > 33 indicates homology		 ALTQIEAK + Deamidated (NQ)
910 449.7424 897.4702 897.4668 3.81 0 23 0.52 +1Score > 39 indicates identity
Score > 33 indicates homology		 QQLTGHSK
376 324.1671 646.3196 646.3187 1.47 0 23 0.75 +1Score > 44 indicates identity
Score > 35 indicates homology		 SWQAR
1685 615.3513 1228.6880 1228.6663 17.7 1 23 0.76 +1Score > 40 indicates identity
Score > 35 indicates homology		 ISDAVELVDIR
647 388.6938 775.3730 775.3712 2.42 0 23 0.97 +1Score > 43 indicates identity
Score > 36 indicates homology		 ANSDIQK + Deamidated (NQ)
1892 439.8838 1316.6296 1316.6208 6.66 1 23 0.62 +1Score > 41 indicates identity
Score > 34 indicates homology		 SGNGVADILDEAR + Deamidated (NQ)
352 315.7138 629.4130 629.4112 2.92 0 23 0.59 +1Score > 38 indicates identity
Score > 33 indicates homology		 IVGTIK
1625 606.3438 1210.6730 1210.6822 -7.56 1 23 0.46 +1Score > 38 indicates identity
Score > 32 indicates homology		 QSILLRYYR
639 772.4707 771.4634 771.4715 -10.5 1 23 0.1 +1Score > 42 indicates identity
Score > 26 indicates homology		 RLVATGR
503 352.2222 702.4298 702.4276 3.23 1 23 0.33 +1Score > 38 indicates identity
Score > 31 indicates homology		 KIDVTK
555 366.2191 730.4236 730.4337 -13.8 0 23 0.15 +1Score > 43 indicates identity
Score > 27 indicates homology		 VATTALR
1085 485.7654 969.5162 969.4992 17.6 1 23 0.2 +1Score > 39 indicates identity
Score > 29 indicates homology		 VNPQARQR + 2 Deamidated (NQ)
2601 546.3296 1635.9670 1635.9712 -2.58 1 23 0.052 +1Score > 30 indicates identity
Score > 23 indicates homology		 GLVLSLPELLGWAIR
1228 1031.5132 1030.5059 1030.5043 1.54 1 23 0.3 +1Score > 41 indicates identity
Score > 30 indicates homology		 TVQESPQSR
291 602.3527 601.3454 601.3435 3.19 0 23 0.53 +1Score > 45 indicates identity
Score > 33 indicates homology		 LSAQIA
673 395.2362 788.4578 788.4578 0.029 0 23 0.094 +1Score > 39 indicates identity
Score > 25 indicates homology		 IMLGALR + Oxidation (M)
1184 337.8674 1010.5804 1010.5913 -10.8 0 23 0.71 +1Score > 36 indicates identity
Score > 34 indicates homology		 QWILNPLK
1569 593.3385 1184.6624 1184.6765 -11.8 0 23 0.093 +1Score > 40 indicates identity
Score > 25 indicates homology		 AVTAAQAVLDVK
565 367.7329 733.4512 733.4486 3.56 0 23 0.6 +1Score > 35 indicates identity
Score > 33 indicates homology		 VYAILR
983 310.5127 928.5163 928.5130 3.49 1 23 0.52 +1Score > 43 indicates identity
Score > 32 indicates homology		 IYEHVLR
993 468.2763 934.5380 934.5348 3.44 1 23 0.82 +1Score > 37 indicates identity
Score > 34 indicates homology		 GRHPLLDK
501 352.2220 702.4294 702.4276 2.66 1 23 0.8 +1Score > 38 indicates identity
Score > 34 indicates homology		 VTKQVK + Deamidated (NQ)
258 569.3062 568.2989 568.2969 3.55 0 23 0.18 +1Score > 38 indicates identity
Score > 28 indicates homology		 DVHAK
334 311.6699 621.3252 621.3235 2.88 0 23 0.95 +1Score > 40 indicates identity
Score > 35 indicates homology		 AGGVYR
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