MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 101–200 (out of 3786)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
461 689.3954 688.3881 688.3868 1.95 1 28 0.65 +1Score > 43 indicates identity
Score > 39 indicates homology		 KQTVGR + Deamidated (NQ)
2967 919.9586 1837.9026 1837.9172 -7.91 1 28 0.2 +1Score > 40 indicates identity
Score > 34 indicates homology		 VYSMFKSVILATFTSE + Oxidation (M)
450 343.2094 684.4042 684.3919 18.1 0 28 0.43 +1Score > 37 indicates identity ATAGIPR
870 440.2537 878.4928 878.4861 7.65 0 28 0.42 +1Score > 37 indicates identity YILNSIR + Deamidated (NQ)
1164 501.2883 1000.5620 1000.5553 6.76 1 28 0.65 +1Score > 41 indicates identity
Score > 39 indicates homology		 KADAALDAVK
787 848.3914 847.3841 847.3672 20.0 1 28 0.42 +1Score > 41 indicates identity
Score > 37 indicates homology		 DDNVETR
354 631.3680 630.3607 630.3701 -14.8 0 28 0.98 +1Score > 44 indicates identity
Score > 41 indicates homology		 ATAAGIK
2346 510.6184 1528.8334 1528.8072 17.1 0 28 0.11 +1Score > 39 indicates identity
Score > 31 indicates homology		 GGVMPIAPGYLAQVR + Deamidated (NQ)
644 775.4341 774.4268 774.4348 -10.3 1 28 0.7 +1Score > 43 indicates identity
Score > 39 indicates homology		 NGISRTK
680 395.7201 789.4256 789.4345 -11.2 0 28 0.83 +1Score > 41 indicates identity
Score > 40 indicates homology		 TSQVISR
1581 595.2982 1188.5818 1188.5696 10.3 1 28 0.2 +1Score > 42 indicates identity
Score > 33 indicates homology		 LTESTMLIHE + Oxidation (M)
521 716.3964 715.3891 715.3977 -12.0 0 28 0.36 +1Score > 44 indicates identity
Score > 36 indicates homology		 QVSVAGR
2072 350.7104 1398.8125 1398.8194 -4.97 1 28 0.28 +1Score > 35 indicates identity DLTLLRTDLALR
527 360.1832 718.3518 718.3650 -18.3 0 28 0.32 +1Score > 44 indicates identity
Score > 36 indicates homology		 TGIWDK
424 673.3990 672.3917 672.3919 -0.20 0 28 0.31 +1Score > 43 indicates identity
Score > 35 indicates homology		 SVALAGR
610 381.2067 760.3988 760.3967 2.89 1 28 0.18 +1Score > 43 indicates identity
Score > 33 indicates homology		 AQTEALK + Deamidated (NQ)
2098 470.5636 1408.6690 1408.6616 5.22 1 28 0.058 +1Score > 41 indicates identity
Score > 28 indicates homology		 SASGDNMSKIIDR + Oxidation (M)
1679 614.8379 1227.6612 1227.6823 -17.1 0 28 0.93 +1Score > 40 indicates identity TNQALGQALLAK + Deamidated (NQ)
2055 464.2607 1389.7603 1389.7504 7.14 1 28 0.083 +1Score > 39 indicates identity
Score > 30 indicates homology		 GLFVAISNQEIAK + Deamidated (NQ)
1240 1035.5875 1034.5802 1034.5913 -10.7 1 28 0.17 +1Score > 38 indicates identity
Score > 32 indicates homology		 FAKGLPGFAK
2218 491.9369 1472.7889 1472.7810 5.37 1 28 0.92 +1Score > 40 indicates identity LCSALGELGIWVR
457 345.1963 688.3780 688.3868 -12.7 0 28 0.26 +1Score > 44 indicates identity
Score > 34 indicates homology		 VGTASVR
871 440.2544 878.4942 878.4861 9.24 0 28 0.48 +1Score > 37 indicates identity YILNSIR + Deamidated (NQ)
368 322.2112 642.4078 642.4064 2.20 1 28 0.37 +1Score > 42 indicates identity
Score > 36 indicates homology		 IAKLAQ
301 603.3345 602.3272 602.3275 -0.51 1 28 0.93 +1Score > 46 indicates identity
Score > 40 indicates homology		 EIDVK
451 343.2100 684.4054 684.3919 19.9 0 28 0.24 +1Score > 37 indicates identity
Score > 34 indicates homology		 ATAGIPR
558 732.3985 731.3912 731.3926 -1.85 0 28 0.32 +1Score > 43 indicates identity
Score > 35 indicates homology		 QSIASAR
317 307.7048 613.3950 613.3911 6.36 0 28 0.52 +1Score > 40 indicates identity
Score > 37 indicates homology		 IGGVLR
293 602.3539 601.3466 601.3435 5.19 0 28 0.14 +1Score > 45 indicates identity
Score > 31 indicates homology		 LSAQIA
453 344.1938 686.3730 686.3711 2.83 0 28 0.47 +1Score > 44 indicates identity
Score > 37 indicates homology		 IAADAAR
1033 474.7683 947.5220 947.5110 11.7 0 27 0.95 +1Score > 40 indicates identity GMISNLLGK + Oxidation (M)
918 900.5593 899.5520 899.5626 -11.8 0 27 0.27 +1Score > 37 indicates identity
Score > 34 indicates homology		 CILLLLR
1763 629.8564 1257.6982 1257.6816 13.2 0 27 0.66 +1Score > 39 indicates identity
Score > 38 indicates homology		 LVITQGALSQVE + Deamidated (NQ)
924 452.2491 902.4836 902.4934 -10.8 1 27 0.59 +1Score > 42 indicates identity
Score > 37 indicates homology		 TARDAITR
670 394.2219 786.4292 786.4235 7.28 0 27 0.079 +1Score > 42 indicates identity
Score > 29 indicates homology		 ANAQIIR + 2 Deamidated (NQ)
1653 609.8664 1217.7182 1217.7019 13.4 1 27 0.067 +1Score > 34 indicates identity
Score > 28 indicates homology		 FANTLLELGIK
2895 599.3328 1794.9766 1794.9727 2.17 0 27 0.37 +1Score > 37 indicates identity
Score > 35 indicates homology		 IQNIVASANLGKPLNLE + 2 Deamidated (NQ)
1241 518.2978 1034.5810 1034.6012 -19.5 1 27 0.11 +1Score > 38 indicates identity
Score > 30 indicates homology		 IEIYIVGTK
523 359.6971 717.3796 717.3769 3.78 0 27 0.53 +1Score > 44 indicates identity
Score > 37 indicates homology		 NTGLASR
1155 499.3083 996.6020 996.5855 16.6 1 27 0.069 +1Score > 35 indicates identity
Score > 28 indicates homology		 EVIAIDLPK
828 430.7226 859.4306 859.4399 -10.8 1 27 0.8 +1Score > 43 indicates identity
Score > 39 indicates homology		 LIEQSNR + Deamidated (NQ)
251 559.3215 558.3142 558.3238 -17.1 1 27 0.79 +1Score > 45 indicates identity
Score > 38 indicates homology		 AKGGAR
861 438.7518 875.4890 875.4865 2.93 1 27 0.17 +1Score > 40 indicates identity
Score > 32 indicates homology		 LFAEQIR
590 377.2173 752.4200 752.4181 2.62 0 27 0.13 +1Score > 38 indicates identity
Score > 31 indicates homology		 ALDVHAK
781 422.7499 843.4852 843.4702 17.9 1 27 0.56 +1Score > 43 indicates identity
Score > 37 indicates homology		 IESLSPAK
1266 349.8415 1046.5027 1046.5178 -14.5 1 27 0.073 +1Score > 41 indicates identity
Score > 28 indicates homology		 KAQVQANMR + 2 Deamidated (NQ)
316 307.6981 613.3816 613.3911 -15.5 0 27 0.8 +1Score > 38 indicates identity IGGVLR
899 894.4703 893.4630 893.4607 2.64 1 27 0.71 +1Score > 41 indicates identity
Score > 38 indicates homology		 LSGELYGR
2064 699.8879 1397.7612 1397.7514 7.04 1 27 0.66 +1Score > 39 indicates identity
Score > 37 indicates homology		 VLALLNDNLQRE + Deamidated (NQ)
1095 486.7659 971.5172 971.5148 2.49 0 27 0.92 +1Score > 42 indicates identity
Score > 39 indicates homology		 SAGLQVQNR
1675 613.8478 1225.6810 1225.6779 2.59 1 27 0.89 +1Score > 39 indicates identity
Score > 39 indicates homology		 QLVAELQGNVR
723 407.7443 813.4740 813.4596 17.8 1 26 0.4 +1Score > 41 indicates identity
Score > 35 indicates homology		 KPQELAK + Deamidated (NQ)
1860 651.3582 1300.7018 1300.6874 11.1 0 26 0.24 +1Score > 41 indicates identity
Score > 33 indicates homology		 VLSDAIVDGANVK + Deamidated (NQ)
1166 502.2827 1002.5508 1002.5346 16.2 0 26 0.4 +1Score > 41 indicates identity
Score > 35 indicates homology		 VTNVAGINSK + Deamidated (NQ)
1537 586.8329 1171.6512 1171.6448 5.49 1 26 0.41 +1Score > 40 indicates identity
Score > 35 indicates homology		 QLGLINEAVSK + Deamidated (NQ)
1609 601.3404 1200.6662 1200.6503 13.3 1 26 0.91 +1Score > 40 indicates identity
Score > 38 indicates homology		 SWALVDILER
1380 554.8003 1107.5860 1107.6077 -19.5 0 26 0.17 +1Score > 40 indicates identity
Score > 31 indicates homology		 FNVIIPSYR
1523 584.7748 1167.5350 1167.5482 -11.2 1 26 0.48 +1Score > 40 indicates identity
Score > 36 indicates homology		 VDAQVFEMTK + Deamidated (NQ)
2478 793.4319 1584.8492 1584.8512 -1.21 0 26 0.94 +1Score > 39 indicates identity
Score > 38 indicates homology		 TVILWDVAQGQVVR + 2 Deamidated (NQ)
640 387.2120 772.4094 772.4079 2.01 1 26 0.84 +1Score > 43 indicates identity
Score > 38 indicates homology		 IQVQER + Deamidated (NQ)
1201 339.5150 1015.5232 1015.5298 -6.51 1 26 0.91 +1Score > 42 indicates identity
Score > 38 indicates homology		 AAAATLNEVR + Deamidated (NQ)
1524 584.7752 1167.5358 1167.5482 -10.6 1 26 0.21 +1Score > 40 indicates identity
Score > 32 indicates homology		 VDAQVFEMTK + Deamidated (NQ)
829 430.7473 859.4800 859.4651 17.4 0 26 0.44 +1Score > 42 indicates identity
Score > 35 indicates homology		 IVDSNIAK + Deamidated (NQ)
1629 404.5733 1210.6981 1210.7186 -17.0 1 26 0.62 +1Score > 36 indicates identity KWVGRPLIDK
1421 564.8255 1127.6364 1127.6186 15.8 1 26 0.16 +1Score > 40 indicates identity
Score > 31 indicates homology		 EIVDALVGANK
229 519.3157 518.3084 518.3064 3.91 0 26 0.41 +1Score > 35 indicates identity ATISK
1682 614.8384 1227.6622 1227.6459 13.3 1 26 0.85 +1Score > 40 indicates identity
Score > 38 indicates homology		 QAQQLLELQR + 2 Deamidated (NQ)
1244 1035.5889 1034.5816 1034.6012 -18.9 1 26 0.49 +1Score > 38 indicates identity
Score > 35 indicates homology		 IEIYIVGTK
426 673.4005 672.3932 672.3918 2.05 1 26 0.85 +1Score > 43 indicates identity
Score > 38 indicates homology		 KQIQR + Deamidated (NQ)
1016 473.2499 944.4852 944.4815 4.01 1 26 0.5 +1Score > 43 indicates identity
Score > 35 indicates homology		 VEQIVNDK + Deamidated (NQ)
920 451.2603 900.5060 900.4916 16.0 1 26 0.83 +1Score > 42 indicates identity
Score > 38 indicates homology		 VIDNIAKE
290 301.6797 601.3448 601.3548 -16.5 0 26 0.14 +1Score > 45 indicates identity
Score > 30 indicates homology		 AGVSIR
1091 486.7554 971.4962 971.5036 -7.55 1 26 0.45 +1Score > 41 indicates identity
Score > 35 indicates homology		 VQLEQAGAR + Deamidated (NQ)
902 894.4717 893.4644 893.4607 4.21 1 26 0.53 +1Score > 41 indicates identity
Score > 36 indicates homology		 GISEGLYR
2075 467.9247 1400.7523 1400.7632 -7.78 1 26 0.16 +1Score > 40 indicates identity
Score > 30 indicates homology		 NMCKLRPLLQK + Deamidated (NQ)
320 308.1833 614.3520 614.3500 3.35 0 26 0.23 +1Score > 40 indicates identity
Score > 32 indicates homology		 AGGALAR
249 558.3626 557.3553 557.3537 2.94 0 26 0.84 +1Score > 40 indicates identity
Score > 37 indicates homology		 AGAVLK
1771 631.8527 1261.6908 1261.6918 -0.73 1 26 0.17 +1Score > 40 indicates identity
Score > 31 indicates homology		 FELIQLITQR + 2 Deamidated (NQ)
1307 535.8463 1069.6780 1069.6648 12.4 0 26 0.17 +1Score > 32 indicates identity
Score > 30 indicates homology		 LIILPQAFR
1600 400.5580 1198.6522 1198.6499 1.91 0 26 0.57 +1Score > 40 indicates identity
Score > 36 indicates homology		 LGQFFTFLAR
1652 609.8645 1217.7144 1217.7019 10.3 1 26 0.41 +1Score > 34 indicates identity FANTLLELGIK
1815 640.8511 1279.6876 1279.6628 19.4 0 26 0.33 +1Score > 39 indicates identity
Score > 33 indicates homology		 LLMLMSGAGSIR + 2 Oxidation (M)
1858 434.2455 1299.7147 1299.7034 8.66 1 26 0.099 +1Score > 40 indicates identity
Score > 28 indicates homology		 QVGGLDEIILSR + Deamidated (NQ)
1249 346.8500 1037.5282 1037.5115 16.0 0 26 0.24 +1Score > 40 indicates identity
Score > 32 indicates homology		 GGPGGGRPGGGGR
2401 778.4505 1554.8864 1554.8869 -0.27 1 26 0.38 +1Score > 36 indicates identity
Score > 34 indicates homology		 DKIIVQSVPDVTLK + Deamidated (NQ)
738 412.7366 823.4586 823.4664 -9.43 0 26 0.091 +1Score > 38 indicates identity
Score > 28 indicates homology		 HLASLAGR
1199 339.4990 1015.4752 1015.4822 -6.90 1 25 0.062 +1Score > 40 indicates identity
Score > 26 indicates homology		 QPLNTGEQK + 2 Deamidated (NQ)
1253 521.7903 1041.5660 1041.5818 -15.2 0 25 0.75 +1Score > 40 indicates identity
Score > 37 indicates homology		 VGADNNLLVK
255 561.3061 560.2988 560.2918 12.5 0 25 0.42 +1Score > 44 indicates identity
Score > 34 indicates homology		 DATVR
1180 337.5266 1009.5580 1009.5379 19.9 0 25 0.06 +1Score > 37 indicates identity
Score > 26 indicates homology		 TAPGAPMLPR
556 366.2197 730.4248 730.4337 -12.2 0 25 0.51 +1Score > 43 indicates identity
Score > 35 indicates homology		 VATTALR
1225 343.8705 1028.5897 1028.5866 2.99 1 25 0.3 +1Score > 40 indicates identity
Score > 33 indicates homology		 EVNLAVSGIK
374 322.7214 643.4282 643.4269 2.15 0 25 0.084 +1Score > 33 indicates identity
Score > 27 indicates homology		 LSVVVK
1741 625.3282 1248.6418 1248.6237 14.5 1 25 0.73 +1Score > 41 indicates identity
Score > 36 indicates homology		 QFALINETALE + Deamidated (NQ)
2216 491.9366 1472.7880 1472.8100 -14.9 0 25 0.67 +1Score > 40 indicates identity
Score > 36 indicates homology		 HGLPPTAGLGIGIDR
569 370.7258 739.4370 739.4341 4.03 0 25 0.17 +1Score > 35 indicates identity
Score > 30 indicates homology		 AVAVAGPR
606 380.7210 759.4274 759.4126 19.5 0 25 0.31 +1Score > 42 indicates identity
Score > 33 indicates homology		 INSADLK
1128 330.5143 988.5211 988.5189 2.18 0 25 0.82 +1Score > 42 indicates identity
Score > 37 indicates homology		 SQIITDGVR + Deamidated (NQ)
1273 527.7215 1053.4284 1053.4251 3.21 0 25 0.29 +1Score > 37 indicates identity
Score > 32 indicates homology		 QFNQSVSNE + 2 Deamidated (NQ)
713 403.2311 804.4476 804.4527 -6.33 0 25 0.27 +1Score > 41 indicates identity
Score > 32 indicates homology		 VSAMILR + Oxidation (M)
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