MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 3786)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3215 671.0410 2010.1012 2010.0786 11.3 1 10 0.31 +1Score > 36 indicates identity
Score > 17 indicates homology		 LVHDIIDLIYLNGLRNE + Deamidated (NQ)
3901 617.5867 2466.3177 2466.3040 5.57 1 10 0.2 +1Score > 35 indicates identity
Score > 15 indicates homology		 TDLKNVAGTVYSNIIQGMATLIK + Deamidated (NQ); Oxidation (M)
1130 495.2806 988.5466 988.5342 12.6 0 10 0.72 +1Score > 40 indicates identity
Score > 21 indicates homology		 LYGDPLGVR
2724 568.2870 1701.8392 1701.8495 -6.08 0 10 0.69 +1Score > 41 indicates identity
Score > 21 indicates homology		 IAVGMGADVTVIDLSPE + Oxidation (M)
1200 339.4990 1015.4752 1015.4934 -18.0 0 10 0.97 +1Score > 40 indicates identity
Score > 22 indicates homology		 GAADSASVPNK
1640 405.8918 1214.6536 1214.6619 -6.84 1 10 0.89 +1Score > 41 indicates identity
Score > 22 indicates homology		 LLETGANQTIR
1821 640.8891 1279.7636 1279.7863 -17.7 1 10 0.33 +1Score > 33 indicates identity
Score > 17 indicates homology		 GIPAVNLIKIDK
2682 558.6186 1672.8340 1672.8460 -7.21 1 10 0.62 +1Score > 41 indicates identity
Score > 20 indicates homology		 GYNAVYFKAALSGALE
2689 558.9863 1673.9371 1673.9134 14.1 1 10 0.55 +1Score > 35 indicates identity
Score > 20 indicates homology		 VSLQNLSMVLRQLR + 2 Deamidated (NQ); Oxidation (M)
228 517.3118 516.3045 516.3020 4.91 0 10 0.51 +1Score > 43 indicates identity
Score > 19 indicates homology		 IAGTR
1862 651.3628 1300.7110 1300.7140 -2.24 0 10 0.79 +1Score > 40 indicates identity
Score > 21 indicates homology		 GVVVAFGGQTPLR + Deamidated (NQ)
2322 757.9529 1513.8912 1513.8868 2.94 1 10 0.84 +1Score > 32 indicates identity
Score > 21 indicates homology		 IIDNFPGKSILGIK
3101 964.4917 1926.9688 1926.9397 15.1 1 10 0.84 +1Score > 39 indicates identity
Score > 21 indicates homology		 KMDIIDIDYFGNVDLR + Deamidated (NQ)
3124 971.5430 1941.0714 1941.0935 -11.4 0 10 0.58 +1Score > 35 indicates identity
Score > 20 indicates homology		 LLIVDRPIDQIAPVIHE + Deamidated (NQ)
1562 591.3680 1180.7214 1180.7067 12.5 1 10 0.85 +1Score > 31 indicates identity
Score > 21 indicates homology		 EIIQVLQLPK + Deamidated (NQ)
760 419.6913 837.3680 837.3769 -10.6 0 10 0.74 +1Score > 40 indicates identity
Score > 21 indicates homology		 WFNNTR + Deamidated (NQ)
3746 775.4258 2323.2556 2323.2172 16.5 0 10 0.28 +1Score > 35 indicates identity
Score > 17 indicates homology		 IVQDFVSNHVSNAPSLAAALIR + 2 Deamidated (NQ)
629 384.7068 767.3990 767.4038 -6.22 0 10 0.33 +1Score > 37 indicates identity
Score > 17 indicates homology		 IASHGQR
1837 430.8860 1289.6362 1289.6173 14.7 1 10 0.6 +1Score > 42 indicates identity
Score > 20 indicates homology		 MTAIEPQNLEK + Deamidated (NQ); Oxidation (M)
3213 671.0215 2010.0427 2010.0520 -4.66 0 10 0.71 +1Score > 39 indicates identity
Score > 21 indicates homology		 VSIQQILGNNDEPLINLK + 3 Deamidated (NQ)
1631 405.2042 1212.5908 1212.6098 -15.7 1 10 0.64 +1Score > 40 indicates identity
Score > 20 indicates homology		 QEPQLELSNR
2571 812.4291 1622.8436 1622.8338 6.08 0 10 0.47 +1Score > 40 indicates identity
Score > 19 indicates homology		 TYIVASVVGLINNMN + Oxidation (M)
3098 641.9767 1922.9083 1922.9407 -16.9 1 10 0.36 +1Score > 40 indicates identity
Score > 18 indicates homology		 NIMLSTNIQNFDDLRK + 2 Deamidated (NQ)
2323 505.6390 1513.8952 1513.8789 10.7 1 10 0.39 +1Score > 31 indicates identity
Score > 18 indicates homology		 LISILLNKMLPDK + Deamidated (NQ); Oxidation (M)
2087 703.3394 1404.6642 1404.6779 -9.73 1 10 0.34 +1Score > 41 indicates identity
Score > 17 indicates homology		 AAARMADDLASAAR + Oxidation (M)
4535 826.6685 3302.6449 3302.6089 10.9 1 10 1 +1Score > 36 indicates identity
Score > 22 indicates homology		 VVQQIVHQAGAGHQIIVQNVTMDQETALGSE + 3 Deamidated (NQ)
1577 594.8245 1187.6344 1187.6397 -4.46 1 10 0.94 +1Score > 41 indicates identity
Score > 22 indicates homology		 ALQKGLTQLSE + Deamidated (NQ)
2001 456.2299 1365.6679 1365.6863 -13.5 1 10 0.8 +1Score > 41 indicates identity
Score > 21 indicates homology		 RSCQFLQLWK + Deamidated (NQ)
3423 712.7112 2135.1118 2135.0833 13.3 1 10 0.24 +1Score > 38 indicates identity
Score > 16 indicates homology		 LVHHDIIDALENMSRPFK + Deamidated (NQ)
4533 826.1644 3300.6285 3300.6409 -3.75 1 10 0.3 +1Score > 36 indicates identity
Score > 17 indicates homology		 VVQQIVHQAGAGHQIIVQNVTMDQETALGSE + Deamidated (NQ)
1996 682.3797 1362.7448 1362.7256 14.2 0 10 0.99 +1Score > 38 indicates identity
Score > 22 indicates homology		 VLIDEPGSQHIR
1428 565.8311 1129.6476 1129.6343 11.8 1 10 0.4 +1Score > 38 indicates identity
Score > 18 indicates homology		 TAKAIAVTVQE
2619 823.9197 1645.8248 1645.7995 15.4 0 10 0.65 +1Score > 41 indicates identity
Score > 20 indicates homology		 EPRPGLAQDPPGCPR
2994 928.4589 1854.9032 1854.8748 15.3 0 9 0.86 +1Score > 40 indicates identity
Score > 21 indicates homology		 EPSGGNNWAPLQAGDTLK + Deamidated (NQ)
2407 520.2877 1557.8413 1557.8362 3.23 1 9 0.52 +1Score > 39 indicates identity
Score > 19 indicates homology		 AIVTNVAGTTRDIVE
1522 583.8332 1165.6518 1165.6455 5.44 0 9 0.74 +1Score > 37 indicates identity
Score > 21 indicates homology		 QGLAVNKPDPK
3443 717.0587 2148.1543 2148.1356 8.69 0 9 0.86 +1Score > 36 indicates identity
Score > 21 indicates homology		 NMGTLALMGLIMTLLAQASAK + Deamidated (NQ)
1790 636.8413 1271.6680 1271.6907 -17.8 1 9 0.9 +1Score > 41 indicates identity
Score > 21 indicates homology		 LKPEIQCVSAK
2489 530.6471 1588.9195 1588.9011 11.6 1 9 0.26 +1Score > 32 indicates identity
Score > 16 indicates homology		 VTQKFQLTLPGVMK
3971 636.8063 2543.1961 2543.2292 -13.0 1 9 0.34 +1Score > 38 indicates identity
Score > 17 indicates homology		 NVWAANGSNEILQQLLQAFGGPGR + 4 Deamidated (NQ)
3427 1068.6077 2135.2008 2135.1739 12.6 1 9 0.37 +1Score > 32 indicates identity
Score > 18 indicates homology		 WLPSLNPLDINVVVTGKDR
3914 826.4437 2476.3093 2476.3332 -9.68 1 9 0.14 +1Score > 36 indicates identity
Score > 14 indicates homology		 GLSCAQARIQIVADLQAHGLLSR
3489 725.3710 2173.0912 2173.0804 4.97 1 9 0.69 +1Score > 39 indicates identity
Score > 20 indicates homology		 DKIHHVDSLHQLFSAISPE + Deamidated (NQ)
466 346.2126 690.4106 690.4098 1.20 1 9 0.58 +1Score > 37 indicates identity
Score > 20 indicates homology		 KGLVMK + Oxidation (M)
2908 601.6086 1801.8040 1801.7941 5.47 1 9 0.42 +1Score > 38 indicates identity
Score > 18 indicates homology		 TDFVARNDAFQDMVR + 2 Deamidated (NQ); Oxidation (M)
406 335.7111 669.4076 669.4061 2.27 0 9 0.78 +1Score > 34 indicates identity
Score > 21 indicates homology		 VIPDVK
1844 647.3248 1292.6350 1292.6360 -0.77 1 9 0.9 +1Score > 41 indicates identity
Score > 21 indicates homology		 FTLITNAENNR + Deamidated (NQ)
3061 947.4996 1892.9846 1892.9731 6.08 1 9 0.55 +1Score > 39 indicates identity
Score > 19 indicates homology		 GGVVVASKDGSIVVDYSIE
3547 550.2747 2197.0697 2197.0434 12.0 0 9 0.64 +1Score > 39 indicates identity
Score > 20 indicates homology		 DHTKPVQQVLADMTNGGVDR + Deamidated (NQ); Oxidation (M)
2552 538.9537 1613.8393 1613.8373 1.24 1 9 0.43 +1Score > 40 indicates identity
Score > 18 indicates homology		 AKNADLTAADLDAAVR
3273 1023.5349 2045.0552 2045.0476 3.73 1 9 0.78 +1Score > 39 indicates identity
Score > 21 indicates homology		 MRDSANLAPFAVAGGANVLR + Oxidation (M)
3905 617.8282 2467.2837 2467.2569 10.8 0 9 0.2 +1Score > 37 indicates identity
Score > 15 indicates homology		 GNAVAINAFVPLANMFGYINTLR + 2 Deamidated (NQ)
2499 532.9440 1595.8102 1595.8096 0.36 0 9 0.46 +1Score > 40 indicates identity
Score > 18 indicates homology		 VAIYVNGNYQWLR + Deamidated (NQ)
3104 966.0428 1930.0710 1930.0524 9.69 0 9 0.43 +1Score > 34 indicates identity
Score > 18 indicates homology		 LDPVSISYIDSRPISLR
3184 994.9767 1987.9388 1987.9231 7.92 1 9 0.76 +1Score > 39 indicates identity
Score > 21 indicates homology		 ITSDTEVFTMCTDTIVR
3217 671.3312 2010.9718 2010.9608 5.45 1 9 0.2 +1Score > 40 indicates identity
Score > 15 indicates homology		 AYEADPTSIFGGIIALNME
3644 751.7102 2252.1088 2252.0729 15.9 1 9 0.31 +1Score > 39 indicates identity
Score > 17 indicates homology		 NLNSVAKNVSDASNILLSMDK + 4 Deamidated (NQ); Oxidation (M)
1035 475.2656 948.5166 948.5063 11.0 1 9 0.98 +1Score > 40 indicates identity
Score > 22 indicates homology		 KVLCSTGGK
2817 876.4315 1750.8484 1750.8825 -19.4 0 9 0.57 +1Score > 41 indicates identity
Score > 19 indicates homology		 HLYPLHVVMGAAGGDAK + Oxidation (M)
2904 601.3325 1800.9757 1800.9985 -12.7 1 9 0.77 +1Score > 38 indicates identity
Score > 21 indicates homology		 NIVNLNSGQLFEILVK + Deamidated (NQ)
3055 946.4802 1890.9458 1890.9105 18.7 0 9 0.85 +1Score > 40 indicates identity
Score > 21 indicates homology		 DGAASAAVSAGNTGALMAIAR + Deamidated (NQ); Oxidation (M)
3667 758.4120 2272.2142 2272.2461 -14.0 1 9 0.54 +1Score > 36 indicates identity
Score > 19 indicates homology		 TAVKLPGGQLSCTVTASLQTLK
4572 1117.6505 3349.9297 3349.8762 16.0 1 9 1 +1Score > 24 indicates identity
Score > 22 indicates homology		 EGAVQVFRPLLNNDLIVGAVGVLQFDVVVLR + Deamidated (NQ)
204 489.2797 488.2724 488.2707 3.55 0 9 0.41 +1Score > 44 indicates identity
Score > 18 indicates homology		 AGSVR
4460 1061.5273 3181.5601 3181.5682 -2.55 1 9 0.14 +1Score > 36 indicates identity
Score > 13 indicates homology		 LAMHGTHNMLNSLAAIGVATQMGIADDKIR + Deamidated (NQ); 2 Oxidation (M)
2306 756.9572 1511.8998 1511.8963 2.37 1 9 0.82 +1Score > 31 indicates identity
Score > 21 indicates homology		 NLVIIPIAIAFAKE + Deamidated (NQ)
2084 351.9375 1403.7209 1403.7197 0.84 1 9 0.92 +1Score > 41 indicates identity
Score > 21 indicates homology		 HVYPRNYIVNK + 2 Deamidated (NQ)
264 575.3005 574.2932 574.2823 19.0 0 9 1 +1Score > 45 indicates identity
Score > 22 indicates homology		 AGQGSR
3745 775.3951 2323.1635 2323.1453 7.81 1 9 0.6 +1Score > 39 indicates identity
Score > 19 indicates homology		 FTKMHGLGNDFMVVDLISQR + Oxidation (M)
3468 721.7215 2162.1427 2162.1419 0.38 1 9 0.94 +1Score > 37 indicates identity
Score > 21 indicates homology		 FFLALQNGATLLTSRHSMR
1663 611.3393 1220.6640 1220.6765 -10.2 1 9 0.8 +1Score > 40 indicates identity
Score > 21 indicates homology		 LETVLVHEPGK
3567 736.6973 2207.0701 2207.0746 -2.06 1 9 0.4 +1Score > 39 indicates identity
Score > 18 indicates homology		 QGDYITGTFQVKDLPNANPK + 2 Deamidated (NQ)
2118 708.4079 1414.8012 1414.8031 -1.33 1 9 0.48 +1Score > 37 indicates identity
Score > 18 indicates homology		 VALKDAGLSVSDIK
2802 578.9664 1733.8774 1733.8757 0.98 0 9 0.32 +1Score > 40 indicates identity
Score > 17 indicates homology		 ILQNMADTISLIAGQK + 3 Deamidated (NQ); Oxidation (M)
3182 663.3495 1987.0267 1986.9912 17.9 0 9 0.5 +1Score > 39 indicates identity
Score > 19 indicates homology		 VAIIGYGSQGHAHALNLHE + Deamidated (NQ)
3486 1087.0541 2172.0936 2172.1137 -9.21 1 9 0.41 +1Score > 39 indicates identity
Score > 18 indicates homology		 VVAQLQGVGIPVSMFIDPER + 2 Deamidated (NQ); Oxidation (M)
415 671.3826 670.3753 670.3650 15.4 0 9 0.59 +1Score > 36 indicates identity
Score > 19 indicates homology		 AEPVQK
1122 493.2789 984.5432 984.5352 8.14 0 9 0.78 +1Score > 41 indicates identity
Score > 21 indicates homology		 APVNLTTNR
3690 765.3923 2293.1551 2293.1603 -2.30 1 9 0.51 +1Score > 39 indicates identity
Score > 19 indicates homology		 YHQNRPFLPHSLSPGGTKQK + 2 Deamidated (NQ)
1246 518.2991 1034.5836 1034.5648 18.2 0 9 0.93 +1Score > 38 indicates identity
Score > 21 indicates homology		 IFLNSVVDK + Deamidated (NQ)
3036 627.6689 1879.9849 1879.9747 5.42 1 9 0.54 +1Score > 39 indicates identity
Score > 19 indicates homology		 SIVSLMQMAKTAIAIDR + Deamidated (NQ); 2 Oxidation (M)
2061 465.8959 1394.6659 1394.6466 13.8 0 9 0.74 +1Score > 41 indicates identity
Score > 20 indicates homology		 WQSLLNFNSQR + 3 Deamidated (NQ)
3284 686.0118 2055.0136 2054.9891 11.9 1 9 0.33 +1Score > 40 indicates identity
Score > 17 indicates homology		 FQVTTCKHAGGSPRPPCR
1250 346.8709 1037.5909 1037.5869 3.78 0 9 0.85 +1Score > 35 indicates identity
Score > 21 indicates homology		 QLLSVDVHK
1709 619.3137 1236.6128 1236.5986 11.5 1 9 0.94 +1Score > 41 indicates identity
Score > 21 indicates homology		 QGETLFVNDSK
3943 628.0834 2508.3045 2508.2981 2.55 1 9 0.63 +1Score > 37 indicates identity
Score > 20 indicates homology		 RPGIMFADAELMGIPHTLVIGDR
3811 1193.5892 2385.1638 2385.1787 -6.23 0 9 0.38 +1Score > 39 indicates identity
Score > 17 indicates homology		 WIQQVSLANTSHHQLFMVTK + 2 Deamidated (NQ); Oxidation (M)
3306 692.7194 2075.1364 2075.1164 9.64 1 9 0.14 +1Score > 35 indicates identity
Score > 13 indicates homology		 QITPKPIGVGFGIASPEHAR + Deamidated (NQ)
3206 1004.5870 2007.1594 2007.1405 9.46 1 9 0.26 +1Score > 29 indicates identity
Score > 16 indicates homology		 IVLGIDPALGSLGWAVVAKE
2486 529.9678 1586.8816 1586.8515 18.9 1 9 0.97 +1Score > 37 indicates identity
Score > 21 indicates homology		 VLIDSSNQILETVR + Deamidated (NQ)
2866 593.3176 1776.9310 1776.9509 -11.2 0 9 0.62 +1Score > 39 indicates identity
Score > 19 indicates homology		 AISLAYATGQSLLGLIGE + Deamidated (NQ)
2855 590.6025 1768.7857 1768.8003 -8.26 0 9 0.5 +1Score > 38 indicates identity
Score > 19 indicates homology		 NTSNQAISQNTVFTIE + 3 Deamidated (NQ)
2632 552.2916 1653.8530 1653.8250 16.9 1 9 0.75 +1Score > 40 indicates identity
Score > 20 indicates homology		 EGTDTLLFIDNIFR + Deamidated (NQ)
2675 836.4306 1670.8466 1670.8475 -0.52 1 9 0.35 +1Score > 40 indicates identity
Score > 17 indicates homology		 AVQTLDDLLNAGVEGR + Deamidated (NQ)
2509 534.2753 1599.8041 1599.7749 18.3 1 9 0.9 +1Score > 40 indicates identity
Score > 21 indicates homology		 HGVQMMGQIIEALR + 2 Deamidated (NQ); Oxidation (M)
2514 402.4808 1605.8941 1605.8627 19.5 0 9 0.43 +1Score > 36 indicates identity
Score > 18 indicates homology		 VPQAHGLAFSVPAGVR + Deamidated (NQ)
3236 1012.0257 2022.0368 2022.0278 4.49 1 9 0.26 +1Score > 39 indicates identity
Score > 16 indicates homology		 LMPINKAYNIAAVMDAVR + Deamidated (NQ); 2 Oxidation (M)
3458 718.7184 2153.1334 2153.0926 18.9 1 9 0.41 +1Score > 38 indicates identity
Score > 18 indicates homology		 VNDSVQLDIATSKIMDFIK + Deamidated (NQ); Oxidation (M)
2024 459.9134 1376.7184 1376.6936 18.0 1 9 0.64 +1Score > 40 indicates identity
Score > 20 indicates homology		 INLAEQGYDLNK
2076 701.3840 1400.7534 1400.7333 14.4 0 9 0.63 +1Score > 40 indicates identity
Score > 19 indicates homology		 AVAEPSGALALAGMK + Oxidation (M)
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