MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 3786)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1776 633.8346 1265.6546 1265.6503 3.44 1 12 0.95 +1Score > 40 indicates identity
Score > 24 indicates homology
ITYTLIGENNK + Deamidated (NQ)
2870 593.6107 1777.8103 1777.7842 14.7 0 12 0.31 +1Score > 39 indicates identity
Score > 19 indicates homology
VQIWSCSGGANQQWR + 2 Deamidated (NQ)
348 315.2030 628.3914 628.3908 1.04 1 12 0.65 +1Score > 42 indicates identity
Score > 23 indicates homology
IGKSPK
1350 365.5255 1093.5547 1093.5404 13.1 0 12 0.77 +1Score > 41 indicates identity
Score > 23 indicates homology
NSYLIDNVR + Deamidated (NQ)
1224 343.5320 1027.5742 1027.5662 7.78 1 12 0.69 +1Score > 40 indicates identity
Score > 23 indicates homology
KQSGLPQLR + 2 Deamidated (NQ)
3354 703.0430 2106.1072 2106.0898 8.25 1 12 0.32 +1Score > 38 indicates identity
Score > 20 indicates homology
QADVALIWYFTRPQLER + Deamidated (NQ)
1782 423.2242 1266.6508 1266.6278 18.1 1 12 0.73 +1Score > 40 indicates identity
Score > 23 indicates homology
QFIKNNMVQK + 2 Deamidated (NQ); Oxidation (M)
1899 660.3876 1318.7606 1318.7497 8.33 1 12 0.78 +1Score > 35 indicates identity
Score > 23 indicates homology
FLGTTKDPVTLK
2405 390.2343 1556.9081 1556.9039 2.72 0 12 0.081 +1Score > 33 indicates identity
Score > 14 indicates homology
KPVLAVGNVYQTLR
2910 901.9118 1801.8090 1801.8442 -19.5 1 12 0.76 +1Score > 38 indicates identity
Score > 23 indicates homology
RDLTINAIAQDDQGNR + 3 Deamidated (NQ)
593 379.2141 756.4136 756.4130 0.86 0 12 0.34 +1Score > 39 indicates identity
Score > 20 indicates homology
IVASDPR
1355 548.3361 1094.6576 1094.6560 1.51 1 12 0.99 +1Score > 32 indicates identity
Score > 24 indicates homology
ARVALPQALR + Deamidated (NQ)
3327 696.6777 2087.0113 2086.9816 14.2 1 12 0.86 +1Score > 39 indicates identity
Score > 24 indicates homology
YSLLTNSWSTAKSMNMPR + Deamidated (NQ)
1209 510.7575 1019.5004 1019.5070 -6.39 1 12 0.76 +1Score > 42 indicates identity
Score > 23 indicates homology
RTQQDLMK + Deamidated (NQ)
3269 682.0115 2043.0127 2042.9949 8.70 1 12 0.47 +1Score > 39 indicates identity
Score > 21 indicates homology
LFSLLSDFYLNPTNNER + Deamidated (NQ)
3431 1071.5736 2141.1326 2141.0906 19.7 0 12 0.26 +1Score > 37 indicates identity
Score > 19 indicates homology
AANNAGIPVVFDPVGAGATPFR + Deamidated (NQ)
3805 794.1019 2379.2839 2379.2885 -1.96 0 12 0.31 +1Score > 35 indicates identity
Score > 19 indicates homology
FGIGADGVLFLAKPLHTSLHMR
1520 389.5574 1165.6504 1165.6417 7.47 0 12 0.67 +1Score > 37 indicates identity
Score > 23 indicates homology
SFLMQILGLK + Deamidated (NQ); Oxidation (M)
1950 672.4120 1342.8094 1342.8336 -18.0 1 12 0.33 +1Score > 31 indicates identity
Score > 20 indicates homology
GTKIYLLAPIVR
1984 453.5797 1357.7173 1357.7354 -13.3 1 12 0.7 +1Score > 40 indicates identity
Score > 23 indicates homology
NWTVISKSIPGR + Deamidated (NQ)
1188 507.7917 1013.5688 1013.5645 4.34 1 12 0.48 +1Score > 41 indicates identity
Score > 21 indicates homology
DIIEIIGLE
2172 482.6024 1444.7854 1444.7708 10.1 1 12 0.69 +1Score > 39 indicates identity
Score > 23 indicates homology
RQMVLNSILNQK + 2 Deamidated (NQ)
2058 464.2614 1389.7624 1389.7504 8.65 1 12 0.46 +1Score > 39 indicates identity
Score > 21 indicates homology
GLFVAISNQEIAK + Deamidated (NQ)
4056 663.0619 2648.2185 2648.2581 -14.9 1 12 0.23 +1Score > 37 indicates identity
Score > 18 indicates homology
MPPSPLSSGAFYQRWLGPVLANDE + Deamidated (NQ); Oxidation (M)
1746 313.4205 1249.6529 1249.6601 -5.79 1 12 1 +1Score > 41 indicates identity
Score > 24 indicates homology
MRTVFSQIPR + Oxidation (M)
337 312.1741 622.3336 622.3439 -16.4 0 12 0.54 +1Score > 41 indicates identity
Score > 22 indicates homology
ITFSR
1472 573.3281 1144.6416 1144.6526 -9.53 0 12 0.34 +1Score > 40 indicates identity
Score > 20 indicates homology
GLIVGLIAMNK + Deamidated (NQ); Oxidation (M)
2559 539.3032 1614.8878 1614.8651 14.1 0 12 0.72 +1Score > 38 indicates identity
Score > 23 indicates homology
LSVISGPSTILQMPR + Deamidated (NQ); Oxidation (M)
3742 774.4168 2320.2286 2320.2249 1.58 1 12 0.54 +1Score > 36 indicates identity
Score > 22 indicates homology
QLLQLMIHSVYSNKDVFLR + Deamidated (NQ); Oxidation (M)
2159 720.4009 1438.7872 1438.8143 -18.8 0 12 0.99 +1Score > 38 indicates identity
Score > 24 indicates homology
LNLLPSSVLQNNK
1938 668.8305 1335.6464 1335.6531 -5.00 1 12 0.96 +1Score > 41 indicates identity
Score > 24 indicates homology
TDQYRQVSSPR
2390 776.8952 1551.7758 1551.7892 -8.63 1 12 0.69 +1Score > 41 indicates identity
Score > 23 indicates homology
NAVLKTAFSQAASSR + 2 Deamidated (NQ)
2508 799.9462 1597.8778 1597.9039 -16.3 1 12 0.85 +1Score > 38 indicates identity
Score > 23 indicates homology
VTSAAAELTLLGVTPR
4014 862.4518 2584.3336 2584.3393 -2.22 1 12 0.25 +1Score > 37 indicates identity
Score > 18 indicates homology
VDPVNFKLLSQCIQVVLAAHMGK + 2 Deamidated (NQ); Oxidation (M)
2153 718.3647 1434.7148 1434.7103 3.16 0 12 0.78 +1Score > 41 indicates identity
Score > 23 indicates homology
ADLFPAVSATGSGSR
557 366.2271 730.4396 730.4337 8.10 0 12 0.4 +1Score > 40 indicates identity
Score > 20 indicates homology
VASSLVR
1084 485.7105 969.4064 969.4226 -16.7 0 12 0.96 +1Score > 38 indicates identity
Score > 24 indicates homology
TMDPFTSR + Oxidation (M)
1802 320.1831 1276.7033 1276.6809 17.5 1 12 0.42 +1Score > 38 indicates identity
Score > 20 indicates homology
SLKAMLNGTGIR + Deamidated (NQ); Oxidation (M)
3320 1043.5374 2085.0602 2085.0466 6.55 1 12 0.35 +1Score > 39 indicates identity
Score > 20 indicates homology
YHGLSVGVILNTMHPFER + Oxidation (M)
2290 752.3918 1502.7690 1502.7437 16.8 1 12 0.8 +1Score > 41 indicates identity
Score > 23 indicates homology
LTSDGAGISGGQRQR + Deamidated (NQ)
3670 758.7447 2273.2123 2273.1837 12.5 1 12 0.19 +1Score > 36 indicates identity
Score > 17 indicates homology
KSNFSLAILNVGAPAAGMNAAVR + 2 Deamidated (NQ)
2703 846.8754 1691.7362 1691.7373 -0.64 1 12 0.65 +1Score > 37 indicates identity
Score > 22 indicates homology
QLANGVQATLDNSETE + 3 Deamidated (NQ)
2706 847.4255 1692.8364 1692.8518 -9.07 1 12 0.68 +1Score > 41 indicates identity
Score > 22 indicates homology
NHSRAYVHHLLMAK + Deamidated (NQ); Oxidation (M)
3183 663.6534 1987.9384 1987.9673 -14.6 1 12 0.56 +1Score > 39 indicates identity
Score > 22 indicates homology
QSLNQFMAVYKLNDSSK + Oxidation (M)
664 392.7513 783.4880 783.4854 3.32 0 12 0.58 +1Score > 35 indicates identity
Score > 22 indicates homology
VIVTPGAK
3797 594.0962 2372.3557 2372.3290 11.2 1 12 0.97 +1Score > 30 indicates identity
Score > 24 indicates homology
ILGFMVLKDTQVLVNVWAIGR + Deamidated (NQ)
3804 794.1017 2379.2833 2379.2885 -2.22 0 12 0.14 +1Score > 35 indicates identity
Score > 16 indicates homology
FGIGADGVLFLAKPLHTSLHMR
2484 397.7165 1586.8369 1586.8264 6.62 1 12 0.74 +1Score > 40 indicates identity
Score > 23 indicates homology
VDDTIQTLQQRLR + 2 Deamidated (NQ)
617 382.2102 762.4058 762.4058 0.093 1 12 0.97 +1Score > 43 indicates identity
Score > 24 indicates homology
MLQRAK + Deamidated (NQ); Oxidation (M)
895 894.4288 893.4215 893.4350 -15.1 1 12 0.76 +1Score > 42 indicates identity
Score > 23 indicates homology
MMAEIVGK + Oxidation (M)
2006 456.5700 1366.6882 1366.6914 -2.38 1 12 0.73 +1Score > 40 indicates identity
Score > 23 indicates homology
LYAAMQANIRAK + 2 Deamidated (NQ); Oxidation (M)
4211 948.5518 2842.6336 2842.5891 15.6 1 12 0.12 +1Score > 26 indicates identity
Score > 15 indicates homology
IIITWSRGSTIIPIMIGHTIAIHNGK + Deamidated (NQ)
2287 752.3869 1502.7592 1502.7576 1.08 1 12 0.9 +1Score > 41 indicates identity
Score > 24 indicates homology
TSLTLADIQDAEAR
855 437.7174 873.4202 873.4344 -16.3 1 12 0.45 +1Score > 41 indicates identity
Score > 21 indicates homology
LKHNFSE
1881 437.2229 1308.6469 1308.6343 9.58 1 12 0.81 +1Score > 41 indicates identity
Score > 23 indicates homology
SLNSGKVNSVMR + 2 Deamidated (NQ); Oxidation (M)
3063 632.3490 1894.0252 1894.0160 4.85 1 12 0.8 +1Score > 36 indicates identity
Score > 23 indicates homology
ILHAATSDSLVVLDEVGR
1555 393.8888 1178.6446 1178.6659 -18.1 1 12 0.86 +1Score > 38 indicates identity
Score > 23 indicates homology
KVTVFDTTLR
2564 540.2947 1617.8623 1617.8661 -2.34 1 12 0.67 +1Score > 39 indicates identity
Score > 22 indicates homology
TPGAQLALLQRMYR + Deamidated (NQ)
507 354.1786 706.3426 706.3432 -0.78 0 12 0.84 +1Score > 41 indicates identity
Score > 23 indicates homology
ACITSR
3084 638.9929 1913.9569 1913.9516 2.73 0 12 0.45 +1Score > 40 indicates identity
Score > 21 indicates homology
LLGDDNLAGQADQAAMLAK
473 348.7062 695.3978 695.3966 1.81 0 12 0.42 +1Score > 36 indicates identity
Score > 20 indicates homology
APAAAPAK
3490 725.3712 2173.0918 2173.0750 7.72 1 12 0.96 +1Score > 39 indicates identity
Score > 24 indicates homology
TDSANNGDIVVALIDNEATLK + Deamidated (NQ)
1539 391.5690 1171.6852 1171.6924 -6.21 1 11 0.66 +1Score > 37 indicates identity
Score > 22 indicates homology
AALSGAVNTLKK
3199 667.3309 1998.9709 1998.9721 -0.59 1 11 0.39 +1Score > 40 indicates identity
Score > 20 indicates homology
LLGGHMLDQFLPVRQQE + 3 Deamidated (NQ); Oxidation (M)
3268 680.7225 2039.1457 2039.1415 2.04 1 11 0.53 +1Score > 33 indicates identity
Score > 21 indicates homology
TYLVTVAPLESALLLSGHR
2628 825.9330 1649.8514 1649.8777 -15.9 0 11 0.7 +1Score > 40 indicates identity
Score > 22 indicates homology
AIQNSFFLLINDVR + Deamidated (NQ)
3315 693.7089 2078.1049 2078.0983 3.18 0 11 0.28 +1Score > 37 indicates identity
Score > 18 indicates homology
LNSAVFPGGQGGPLMHIIAAK + Deamidated (NQ)
3596 558.0049 2227.9905 2228.0055 -6.75 1 11 0.19 +1Score > 37 indicates identity
Score > 17 indicates homology
SPYHDACPATQQPSVIQAEK + 2 Deamidated (NQ)
1505 580.3616 1158.7086 1158.7046 3.50 1 11 0.23 +1Score > 31 indicates identity
Score > 18 indicates homology
SLPLLMITKK + Oxidation (M)
1649 609.3906 1216.7666 1216.7431 19.4 1 11 0.25 +1Score > 24 indicates identity
Score > 18 indicates homology
LIEGIVAVYLK
2920 904.4602 1806.9058 1806.9251 -10.7 1 11 0.82 +1Score > 40 indicates identity
Score > 23 indicates homology
SIGVSNFTEDLLATVIE
3607 745.4362 2233.2868 2233.2583 12.8 1 11 0.65 +1Score > 29 indicates identity
Score > 22 indicates homology
SLTRFGFTLIIGGAIGNLIDR
363 321.2035 640.3924 640.3908 2.56 0 11 0.75 +1Score > 35 indicates identity
Score > 23 indicates homology
AVGPLGK
3869 609.2969 2433.1585 2433.1958 -15.3 1 11 0.18 +1Score > 38 indicates identity
Score > 17 indicates homology
ANWAVGQLSEGVTVSDGIMQSLR + Oxidation (M)
1795 637.8120 1273.6094 1273.6037 4.49 1 11 0.67 +1Score > 41 indicates identity
Score > 22 indicates homology
DEINDALINQK + 2 Deamidated (NQ)
2727 568.6255 1702.8547 1702.8374 10.2 1 11 0.51 +1Score > 40 indicates identity
Score > 21 indicates homology
SVAATNADDGSRVVVIE + Deamidated (NQ)
916 450.7831 899.5516 899.5440 8.51 0 11 0.93 +1Score > 37 indicates identity
Score > 24 indicates homology
SLIAGKPSK
510 354.6883 707.3620 707.3636 -2.20 0 11 0.92 +1Score > 43 indicates identity
Score > 24 indicates homology
MTTLAR + Oxidation (M)
761 419.6915 837.3684 837.3657 3.27 1 11 0.76 +1Score > 40 indicates identity
Score > 23 indicates homology
WFNDEK
2480 793.4357 1584.8568 1584.8875 -19.4 1 11 0.95 +1Score > 39 indicates identity
Score > 24 indicates homology
DLPFPKIDTTALVR
3295 687.3577 2059.0513 2059.0143 17.9 1 11 0.83 +1Score > 39 indicates identity
Score > 23 indicates homology
TTDLQTTLQLSMKAIQHE + 2 Deamidated (NQ)
3372 1058.5386 2115.0626 2115.0228 18.8 1 11 0.8 +1Score > 39 indicates identity
Score > 23 indicates homology
ANLESVVMPSATCTVTPPPK + Deamidated (NQ); Oxidation (M)
4041 659.8130 2635.2229 2635.2186 1.62 1 11 0.17 +1Score > 37 indicates identity
Score > 16 indicates homology
GQTVFMGDFPLMTDKGTFIINGTE + Deamidated (NQ); Oxidation (M)
1333 542.7623 1083.5100 1083.5309 -19.2 0 11 0.38 +1Score > 40 indicates identity
Score > 20 indicates homology
IGGSSGGHNGLK + Deamidated (NQ)
3292 1030.5305 2059.0464 2059.0375 4.37 1 11 0.44 +1Score > 39 indicates identity
Score > 20 indicates homology
GDKVQGDGDWKPIVQFLR + 2 Deamidated (NQ)
3409 710.3782 2128.1128 2128.0760 17.3 1 11 0.3 +1Score > 38 indicates identity
Score > 19 indicates homology
ADLIVNTIRSSNSPQIQNR + 3 Deamidated (NQ)
2819 877.4670 1752.9194 1752.9482 -16.4 1 11 0.5 +1Score > 39 indicates identity
Score > 21 indicates homology
IDTALRGASDILAPQGR
2791 866.8898 1731.7650 1731.7807 -9.06 0 11 0.49 +1Score > 39 indicates identity
Score > 21 indicates homology
NDNPLVDMQAMSPTAK + Deamidated (NQ)
3692 765.3952 2293.1638 2293.1240 17.4 1 11 0.5 +1Score > 38 indicates identity
Score > 21 indicates homology
FWLGNDHIHALTAQGTNELR + Deamidated (NQ)
2186 486.5863 1456.7371 1456.7270 6.90 1 11 0.78 +1Score > 40 indicates identity
Score > 23 indicates homology
DRADVTAATQGPVR + Deamidated (NQ)
3822 599.5566 2394.1973 2394.1599 15.6 1 11 0.52 +1Score > 38 indicates identity
Score > 21 indicates homology
LADANAAFPMVLIQIPMYNER + 2 Deamidated (NQ); Oxidation (M)
2406 779.8882 1557.7618 1557.7900 -18.0 1 11 0.93 +1Score > 41 indicates identity
Score > 24 indicates homology
AVDAGPIAFVRDGDR
2882 895.4594 1788.9042 1788.9298 -14.3 0 11 0.66 +1Score > 40 indicates identity
Score > 22 indicates homology
ISFDLNVAQGLALLWE + Deamidated (NQ)
3013 933.9293 1865.8440 1865.8499 -3.14 0 11 0.76 +1Score > 39 indicates identity
Score > 23 indicates homology
LDLMDQGTNAMDILSGR + Deamidated (NQ); Oxidation (M)
3290 1030.0314 2058.0482 2058.0892 -19.9 1 11 0.88 +1Score > 39 indicates identity
Score > 23 indicates homology
LTAIDILTTCAADIQRQR
2921 904.4626 1806.9106 1806.9251 -8.00 1 11 0.42 +1Score > 40 indicates identity
Score > 20 indicates homology
SIGVSNFTEDLLATVIE
3220 671.3344 2010.9814 2011.0197 -19.1 1 11 0.99 +1Score > 40 indicates identity
Score > 24 indicates homology
FSKAASSVGITDAVMSWVR
3238 675.0374 2022.0904 2022.0606 14.7 1 11 0.6 +1Score > 36 indicates identity
Score > 22 indicates homology
GLHARVAQAATTNGSIDLAR + Deamidated (NQ)
1664 408.1897 1221.5473 1221.5626 -12.5 1 11 0.89 +1Score > 40 indicates identity
Score > 23 indicates homology
IEQQQFGQSR + 2 Deamidated (NQ)
1804 426.5758 1276.7056 1276.6888 13.2 1 11 0.88 +1Score > 38 indicates identity
Score > 23 indicates homology
SYRDLNAGILR
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