MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 3786)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4367 1015.8767 3044.6083 3044.5675 13.4 1 13 0.18 +1Score > 34 indicates identity
Score > 19 indicates homology
APQVMSLHVDTLVTKFLNLSSSPSMAVR + Deamidated (NQ); Oxidation (M)
734 411.2452 820.4758 820.4807 -5.89 0 13 0.92 +1Score > 37 indicates identity
Score > 26 indicates homology
LFSSVIR
963 308.5163 922.5271 922.5236 3.76 0 13 0.72 +1Score > 37 indicates identity
Score > 25 indicates homology
QIHGQVIK + Deamidated (NQ)
1330 541.8057 1081.5968 1081.6066 -9.04 1 13 0.51 +1Score > 38 indicates identity
Score > 23 indicates homology
APGVGPKVAMR
2903 901.4946 1800.9746 1800.9410 18.7 1 13 0.9 +1Score > 38 indicates identity
Score > 25 indicates homology
VPNIDKPDSKWINFK + Deamidated (NQ)
2132 712.3597 1422.7048 1422.6991 4.05 0 13 0.65 +1Score > 41 indicates identity
Score > 24 indicates homology
ATSVPGPTQATAPPE
3181 663.3475 1987.0207 1987.0271 -3.23 1 13 0.14 +1Score > 39 indicates identity
Score > 17 indicates homology
FSMPKQVGIDLVQMHLK + Deamidated (NQ); Oxidation (M)
1863 651.3939 1300.7732 1300.7755 -1.69 1 13 0.53 +1Score > 33 indicates identity
Score > 23 indicates homology
LQPYVLKVVNK + Deamidated (NQ)
2198 488.2866 1461.8380 1461.8265 7.87 1 13 0.83 +1Score > 34 indicates identity
Score > 25 indicates homology
MKFIINNNILIK + 2 Deamidated (NQ)
2860 887.4542 1772.8938 1772.8655 16.0 1 13 0.28 +1Score > 40 indicates identity
Score > 20 indicates homology
VNFSVNFKCLSSQIK + 3 Deamidated (NQ)
3321 696.0277 2085.0613 2085.0854 -11.6 1 13 0.42 +1Score > 39 indicates identity
Score > 22 indicates homology
AQPGGLAALQAALADATVEYR
1251 347.2237 1038.6493 1038.6437 5.36 0 13 0.7 +1Score > 33 indicates identity
Score > 24 indicates homology
LLAPGTINIK
382 652.3076 651.3003 651.3010 -1.03 0 13 0.62 +1Score > 40 indicates identity
Score > 24 indicates homology
MTAASR + Oxidation (M)
3651 754.0688 2259.1846 2259.1575 12.0 1 13 0.87 +1Score > 37 indicates identity
Score > 25 indicates homology
NLLSFLRGTFLNNYVPFDK + 2 Deamidated (NQ)
1980 677.8820 1353.7494 1353.7616 -8.97 1 13 0.27 +1Score > 38 indicates identity
Score > 20 indicates homology
VLENLLQVSPSR
3587 741.4016 2221.1830 2221.1590 10.8 0 13 0.45 +1Score > 36 indicates identity
Score > 22 indicates homology
LPTLQNNIPASIQLNIAQDR + 3 Deamidated (NQ)
1106 487.3134 972.6122 972.5968 15.9 1 13 0.42 +1Score > 30 indicates identity
Score > 22 indicates homology
ILKTGISNK
2818 877.4647 1752.9148 1752.8902 14.0 1 13 0.92 +1Score > 39 indicates identity
Score > 25 indicates homology
ILLENGMPVTAHLCGK + Deamidated (NQ)
3107 644.3653 1930.0741 1930.0524 11.2 0 13 0.31 +1Score > 34 indicates identity
Score > 21 indicates homology
QSALVQVFNKPVQTITR + 2 Deamidated (NQ)
3472 722.6935 2165.0587 2165.1005 -19.3 0 13 0.89 +1Score > 39 indicates identity
Score > 25 indicates homology
VLSDVVASQAALHGPYGGVVPE + Deamidated (NQ)
1050 319.8387 956.4943 956.5080 -14.3 1 13 0.73 +1Score > 39 indicates identity
Score > 24 indicates homology
WVTPEVAR
1411 374.5375 1120.5907 1120.5764 12.7 0 13 0.73 +1Score > 41 indicates identity
Score > 25 indicates homology
KPSYNVGINK + 2 Deamidated (NQ)
1254 521.7996 1041.5846 1041.5818 2.69 0 13 0.58 +1Score > 40 indicates identity
Score > 23 indicates homology
LNPTLDTLR
1359 550.3143 1098.6140 1098.6033 9.78 1 13 0.94 +1Score > 40 indicates identity
Score > 26 indicates homology
INGKGLDASPK
1676 410.2275 1227.6607 1227.6459 12.0 1 13 0.85 +1Score > 40 indicates identity
Score > 25 indicates homology
QLEGAGAQVDIK
2909 901.9118 1801.8090 1801.8094 -0.17 1 13 0.9 +1Score > 38 indicates identity
Score > 25 indicates homology
NGSSAKWMGPFYIGDR + Deamidated (NQ); Oxidation (M)
319 615.3588 614.3515 614.3500 2.50 0 13 0.82 +1Score > 39 indicates identity
Score > 25 indicates homology
QIAGAR
1828 643.3604 1284.7062 1284.7150 -6.79 1 13 0.96 +1Score > 40 indicates identity
Score > 26 indicates homology
QGLNQLTLNKR + Deamidated (NQ)
1898 660.3087 1318.6028 1318.6153 -9.47 0 13 0.58 +1Score > 40 indicates identity
Score > 23 indicates homology
TLDISDADWQR
2385 775.4042 1548.7938 1548.8148 -13.5 1 13 0.61 +1Score > 40 indicates identity
Score > 24 indicates homology
QPSVKYATLVSNDK
2424 783.9011 1565.7876 1565.7586 18.5 1 13 0.34 +1Score > 41 indicates identity
Score > 21 indicates homology
GVGSAPQWIHASAER + Deamidated (NQ)
2391 388.9741 1551.8673 1551.8369 19.6 1 13 0.61 +1Score > 36 indicates identity
Score > 24 indicates homology
LHQTLAKVSDDIGR
2130 474.9364 1421.7874 1421.7813 4.27 0 13 0.62 +1Score > 37 indicates identity
Score > 24 indicates homology
AKPLMPGGVNSPVR
311 305.6882 609.3618 609.3598 3.28 0 13 0.13 +1Score > 34 indicates identity
Score > 17 indicates homology
AGVHVK
517 357.7002 713.3858 713.3959 -14.1 0 13 0.5 +1Score > 38 indicates identity
Score > 23 indicates homology
VQQLPK + 2 Deamidated (NQ)
1852 432.9164 1295.7274 1295.7193 6.27 1 13 0.76 +1Score > 37 indicates identity
Score > 25 indicates homology
MSMLSTLLLKK + 2 Oxidation (M)
401 334.2116 666.4086 666.4177 -13.6 1 13 0.3 +1Score > 29 indicates identity
Score > 21 indicates homology
KHVVGK
2898 451.2076 1800.8013 1800.7836 9.85 1 13 0.18 +1Score > 38 indicates identity
Score > 18 indicates homology
AHVNSMDDQLANELNK + 3 Deamidated (NQ)
728 408.7302 815.4458 815.4389 8.53 0 13 0.5 +1Score > 43 indicates identity
Score > 23 indicates homology
DVDAAVVK
2792 866.8900 1731.7654 1731.7807 -8.83 0 13 0.32 +1Score > 39 indicates identity
Score > 21 indicates homology
NDNPLVDMQAMSPTAK + Deamidated (NQ)
3743 775.3907 2323.1503 2323.1400 4.44 0 13 0.6 +1Score > 39 indicates identity
Score > 23 indicates homology
TGIQQTSSMADTQLLVAAVGMGK + Deamidated (NQ); Oxidation (M)
981 464.7128 927.4110 927.3974 14.7 1 13 0.66 +1Score > 38 indicates identity
Score > 24 indicates homology
EFFDQNK + Deamidated (NQ)
994 468.7628 935.5110 935.5076 3.69 1 13 0.62 +1Score > 40 indicates identity
Score > 24 indicates homology
LTAELAYR
1426 565.8258 1129.6370 1129.6165 18.2 0 13 0.41 +1Score > 39 indicates identity
Score > 22 indicates homology
ICLDILQQK
2212 736.3718 1470.7290 1470.7566 -18.7 0 13 0.73 +1Score > 41 indicates identity
Score > 24 indicates homology
NNDILLLQGAGTIE + Deamidated (NQ)
2942 455.7190 1818.8469 1818.8782 -17.2 1 13 0.31 +1Score > 40 indicates identity
Score > 21 indicates homology
STGTPIQLMIENTDQR + Oxidation (M)
3654 755.7490 2264.2252 2264.2012 10.6 1 13 0.11 +1Score > 35 indicates identity
Score > 16 indicates homology
LQNLQHLAITLSAELSLNQR + 3 Deamidated (NQ)
677 395.7085 789.4024 789.3868 19.8 1 13 0.94 +1Score > 43 indicates identity
Score > 25 indicates homology
TSKPKDD
2131 712.3595 1422.7044 1422.6813 16.3 1 13 0.88 +1Score > 41 indicates identity
Score > 25 indicates homology
IENLVFMQQQR + 2 Deamidated (NQ); Oxidation (M)
3228 1010.0074 2018.0002 2017.9878 6.19 1 13 0.4 +1Score > 40 indicates identity
Score > 22 indicates homology
NARIIIMDEPTTALTDVE + Deamidated (NQ); Oxidation (M)
1259 522.3082 1042.6018 1042.6022 -0.37 1 13 0.49 +1Score > 39 indicates identity
Score > 23 indicates homology
VSLNIVEIR + Deamidated (NQ)
1148 332.8549 995.5429 995.5400 2.89 1 13 0.57 +1Score > 37 indicates identity
Score > 23 indicates homology
EGQVTPLPR
1584 595.8448 1189.6750 1189.6859 -9.13 0 13 0.69 +1Score > 37 indicates identity
Score > 24 indicates homology
VISIAWNFLK
2670 835.5033 1668.9920 1668.9661 15.5 1 13 0.65 +1Score > 30 indicates identity
Score > 24 indicates homology
LNIILQGNIILSTEK + Deamidated (NQ)
1083 323.8385 968.4937 968.5039 -10.6 1 13 0.63 +1Score > 39 indicates identity
Score > 24 indicates homology
KHQGTIQR + 2 Deamidated (NQ)
1736 623.3149 1244.6152 1244.6361 -16.7 1 13 0.54 +1Score > 41 indicates identity
Score > 23 indicates homology
QTQNAEGGIISK
1189 507.7920 1013.5694 1013.5757 -6.17 1 13 0.73 +1Score > 41 indicates identity
Score > 24 indicates homology
KDVQLIAVE
3953 840.4351 2518.2835 2518.2904 -2.73 1 13 0.82 +1Score > 38 indicates identity
Score > 25 indicates homology
SGIPVMGHIGLTPQFVNRFGGYR + Oxidation (M)
732 411.2446 820.4746 820.4694 6.35 0 13 0.79 +1Score > 37 indicates identity
Score > 25 indicates homology
LNSFVIK + Deamidated (NQ)
1810 427.2402 1278.6988 1278.6819 13.2 1 13 0.66 +1Score > 38 indicates identity
Score > 24 indicates homology
IESSLIANYLR + Deamidated (NQ)
2288 501.9301 1502.7685 1502.7763 -5.18 0 13 0.68 +1Score > 41 indicates identity
Score > 24 indicates homology
GVLSCIALNLQGTR + 2 Deamidated (NQ)
1337 362.5461 1084.6165 1084.5989 16.2 1 13 0.96 +1Score > 40 indicates identity
Score > 25 indicates homology
TDAPAVTRVR
1727 413.9054 1238.6944 1238.6731 17.2 1 13 0.97 +1Score > 37 indicates identity
Score > 25 indicates homology
QRVAADLPVDR
439 675.3821 674.3748 674.3711 5.49 0 13 0.46 +1Score > 40 indicates identity
Score > 22 indicates homology
ALGSTAR
1277 528.2859 1054.5572 1054.5407 15.7 1 13 0.4 +1Score > 40 indicates identity
Score > 22 indicates homology
NSIETQVHK
1605 600.3735 1198.7324 1198.7285 3.29 1 13 0.63 +1Score > 32 indicates identity
Score > 23 indicates homology
RTLQLVDIIK + Deamidated (NQ)
2317 379.2160 1512.8349 1512.8260 5.88 0 13 0.74 +1Score > 38 indicates identity
Score > 24 indicates homology
LASNVNLSLGVVDGR
602 380.2541 758.4936 758.4836 13.2 1 13 0.87 +1Score > 30 indicates identity
Score > 25 indicates homology
VIKMIR
1288 353.8616 1058.5630 1058.5695 -6.17 0 13 0.62 +1Score > 42 indicates identity
Score > 23 indicates homology
WVINMALGR
550 365.2221 728.4296 728.4181 15.9 0 13 0.8 +1Score > 44 indicates identity
Score > 25 indicates homology
AGLNVVR + Deamidated (NQ)
890 445.7361 889.4576 889.4691 -12.9 1 13 0.73 +1Score > 42 indicates identity
Score > 24 indicates homology
NSPMKAVK + Oxidation (M)
3753 777.7692 2330.2858 2330.2888 -1.31 0 13 0.35 +1Score > 33 indicates identity
Score > 21 indicates homology
LMMIGGAGVVLVVAVGLVLNAMR + 3 Oxidation (M)
515 357.2141 712.4136 712.4119 2.42 0 13 0.54 +1Score > 38 indicates identity
Score > 23 indicates homology
DLAGIPK
1334 542.7883 1083.5620 1083.5560 5.55 0 13 0.67 +1Score > 40 indicates identity
Score > 24 indicates homology
GSAPGQPVTAAK + Deamidated (NQ)
540 364.2180 726.4214 726.4276 -8.46 0 13 0.51 +1Score > 36 indicates identity
Score > 22 indicates homology
GPVLVDK
1215 341.8985 1022.6737 1022.6600 13.3 1 13 0.64 +1Score > 23 indicates identity KVPSRPVLK
566 368.2255 734.4364 734.4327 5.13 0 13 0.74 +1Score > 36 indicates identity
Score > 24 indicates homology
LVFQTK
1750 417.9070 1250.6992 1250.6758 18.7 1 13 0.85 +1Score > 37 indicates identity
Score > 25 indicates homology
SSLLPKLTTYE
2082 468.2631 1401.7675 1401.7867 -13.7 1 13 0.26 +1Score > 39 indicates identity
Score > 20 indicates homology
ESLHLIYSSILK
2299 755.3842 1508.7538 1508.7406 8.81 1 13 0.56 +1Score > 41 indicates identity
Score > 23 indicates homology
DIPEVSCIHNGLR
3395 1061.5760 2121.1374 2121.1504 -6.09 0 13 0.33 +1Score > 36 indicates identity
Score > 21 indicates homology
SLVVLFNSSGLLCSGTLINK
972 464.2732 926.5318 926.5185 14.4 0 13 0.74 +1Score > 38 indicates identity
Score > 24 indicates homology
VLQNSLPR + Deamidated (NQ)
3779 787.4237 2359.2493 2359.2212 11.9 0 13 0.12 +1Score > 36 indicates identity
Score > 16 indicates homology
SLYAAVTNFLLSLGYHLNPHK + 2 Deamidated (NQ)
813 428.7311 855.4476 855.4450 3.07 0 13 0.77 +1Score > 39 indicates identity
Score > 24 indicates homology
LADPDVAR
2893 598.9893 1793.9461 1793.9424 2.04 1 13 0.61 +1Score > 38 indicates identity
Score > 23 indicates homology
YNLYLGGDSIGTRIPR
3136 979.4885 1956.9624 1956.9839 -11.0 1 13 0.18 +1Score > 40 indicates identity
Score > 18 indicates homology
AVRYHNNLAAQPVMQTK + Deamidated (NQ); Oxidation (M)
3803 1190.6464 2379.2782 2379.2885 -4.33 0 13 0.42 +1Score > 35 indicates identity
Score > 22 indicates homology
FGIGADGVLFLAKPLHTSLHMR
1814 427.5644 1279.6714 1279.6812 -7.70 1 13 0.74 +1Score > 40 indicates identity
Score > 24 indicates homology
TWIELYGATVK
3119 646.7034 1937.0884 1937.0768 5.96 0 13 0.23 +1Score > 33 indicates identity
Score > 19 indicates homology
DAIIVGCAQVLALVPGVSR
574 372.2574 742.5002 742.4953 6.70 0 13 0.8 +1Score > 32 indicates identity
Score > 24 indicates homology
VLATVLK
856 874.4814 873.4741 873.4807 -7.58 0 13 0.5 +1Score > 42 indicates identity
Score > 22 indicates homology
TLDVDALK
1896 440.2634 1317.7684 1317.7768 -6.41 1 13 0.39 +1Score > 33 indicates identity
Score > 21 indicates homology
LAAIKSLVNYAR
2765 574.2906 1719.8500 1719.8726 -13.2 0 13 0.77 +1Score > 41 indicates identity
Score > 24 indicates homology
GQIPQHIAIIMDGNGR + Deamidated (NQ)
3275 682.6949 2045.0629 2045.0847 -10.7 1 13 0.94 +1Score > 38 indicates identity
Score > 25 indicates homology
LADREPWAPLLHDIPFR
3195 667.0028 1997.9866 1997.9656 10.5 0 13 0.38 +1Score > 40 indicates identity
Score > 21 indicates homology
LDYLAPMSNNLGYVLAVE + Deamidated (NQ); Oxidation (M)
1051 319.8390 956.4952 956.5039 -9.16 0 13 0.94 +1Score > 39 indicates identity
Score > 25 indicates homology
TLASSVHSR
2418 781.9600 1561.9054 1561.9007 3.04 0 13 0.36 +1Score > 32 indicates identity
Score > 21 indicates homology
IFLFLAILLQQLE + 2 Deamidated (NQ)
2214 736.8672 1471.7198 1471.7267 -4.62 1 13 0.67 +1Score > 40 indicates identity
Score > 24 indicates homology
AAIGGEADQAITQAR + Deamidated (NQ)
1864 651.8620 1301.7094 1301.7231 -10.5 1 13 0.54 +1Score > 40 indicates identity
Score > 23 indicates homology
FKQLSVGPLLAE + Deamidated (NQ)
1366 552.8285 1103.6424 1103.6373 4.71 1 13 0.67 +1Score > 37 indicates identity
Score > 23 indicates homology
LLMKVTDLR + Oxidation (M)
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