MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 3786)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1775 633.3054 1264.5962 1264.5969 -0.51 1 14 0.77 +1Score > 41 indicates identity
Score > 26 indicates homology		 LQAVSAGSMQKE + Deamidated (NQ); Oxidation (M)
2157 479.6106 1435.8100 1435.8371 -18.9 1 14 0.39 +1Score > 36 indicates identity
Score > 23 indicates homology		 RPLPLSRSNLQR
1880 655.3300 1308.6454 1308.6422 2.48 1 14 0.39 +1Score > 42 indicates identity
Score > 23 indicates homology		 KSANNAHQVPDK + Deamidated (NQ)
283 601.3327 600.3254 600.3344 -14.9 0 14 0.97 +1Score > 39 indicates identity
Score > 27 indicates homology		 AGVAQR
1090 324.8389 971.4949 971.4924 2.59 0 14 0.43 +1Score > 40 indicates identity
Score > 23 indicates homology		 QPLTQQQK + 2 Deamidated (NQ)
3697 1150.0922 2298.1698 2298.1300 17.3 1 14 0.41 +1Score > 38 indicates identity
Score > 23 indicates homology		 NPENLINDAPIKPTLIGMSNK + 4 Deamidated (NQ); Oxidation (M)
1617 602.8374 1203.6602 1203.6459 11.9 1 14 0.22 +1Score > 39 indicates identity
Score > 20 indicates homology		 SVSTARQLTLE
1919 663.3424 1324.6702 1324.6551 11.4 1 14 0.44 +1Score > 40 indicates identity
Score > 23 indicates homology		 VYPVVGTVYAEE
575 372.7228 743.4310 743.4290 2.80 0 14 0.91 +1Score > 43 indicates identity
Score > 26 indicates homology		 LTNAGLR
252 559.3238 558.3165 558.3238 -13.0 1 14 0.94 +1Score > 45 indicates identity
Score > 26 indicates homology		 KNAAR
1639 607.8579 1213.7012 1213.7070 -4.76 0 14 0.85 +1Score > 38 indicates identity
Score > 26 indicates homology		 LIGNIPLFAQK + Deamidated (NQ)
3042 471.7322 1882.8997 1882.8818 9.50 0 14 0.37 +1Score > 40 indicates identity
Score > 22 indicates homology		 LCSLNINMHHDPFLR + Deamidated (NQ); Oxidation (M)
2416 781.8832 1561.7518 1561.7770 -16.1 0 14 0.32 +1Score > 41 indicates identity
Score > 22 indicates homology		 GQNVGLIASLTTCAR + 2 Deamidated (NQ)
2627 825.9319 1649.8492 1649.8334 9.58 1 14 0.67 +1Score > 40 indicates identity
Score > 25 indicates homology		 AVLLMAAFELTQQAE + Oxidation (M)
2399 519.3007 1554.8803 1554.8617 11.9 0 14 0.46 +1Score > 36 indicates identity
Score > 23 indicates homology		 VTQLLSLLANVQQE
2338 760.9139 1519.8132 1519.7922 13.8 1 14 0.2 +1Score > 39 indicates identity
Score > 20 indicates homology		 LIANNYKGWIVVE + 2 Deamidated (NQ)
683 397.2098 792.4050 792.4051 -0.092 1 14 0.8 +1Score > 42 indicates identity
Score > 26 indicates homology		 MSGIIEK + Oxidation (M)
3407 1064.5642 2127.1138 2127.0749 18.3 1 14 0.64 +1Score > 38 indicates identity
Score > 25 indicates homology		 IHSTFTIQVEYGTGPHAIR + Deamidated (NQ)
3789 789.7222 2366.1448 2366.1246 8.53 1 14 0.99 +1Score > 39 indicates identity
Score > 27 indicates homology		 QNFISANEIDNMALIMPTATR + Deamidated (NQ); Oxidation (M)
3041 628.6402 1882.8988 1882.9173 -9.85 1 14 0.087 +1Score > 40 indicates identity
Score > 16 indicates homology		 TQQVIRNLNPPSSNWE + Deamidated (NQ)
3167 991.4577 1980.9008 1980.8622 19.5 0 14 0.16 +1Score > 38 indicates identity
Score > 19 indicates homology		 QASSQSMPWLADLVQSSE + 2 Deamidated (NQ); Oxidation (M)
2286 752.3785 1502.7424 1502.7213 14.1 1 14 0.76 +1Score > 41 indicates identity
Score > 25 indicates homology		 NTSKGTVTGQVQGPE + Deamidated (NQ)
2569 541.9511 1622.8315 1622.8450 -8.36 1 14 0.42 +1Score > 40 indicates identity
Score > 23 indicates homology		 MSVLKQDGQFVVTR + Oxidation (M)
1731 621.4031 1240.7916 1240.8118 -16.3 1 14 0.28 +1Score > 24 indicates identity
Score > 21 indicates homology		 KISISLIGGLLK
364 322.1875 642.3604 642.3602 0.44 1 14 0.46 +1Score > 35 indicates identity
Score > 23 indicates homology		 GKWPR
2010 684.3530 1366.6914 1366.6803 8.19 1 14 0.79 +1Score > 40 indicates identity
Score > 26 indicates homology		 TSGFEMVQGLLGK + Deamidated (NQ)
1183 506.2778 1010.5410 1010.5509 -9.72 0 14 0.28 +1Score > 39 indicates identity
Score > 21 indicates homology		 HSGTAALINK
2939 605.9777 1814.9113 1814.9149 -2.01 1 14 0.64 +1Score > 40 indicates identity
Score > 25 indicates homology		 SQLLISAEPIVVSSDEE
973 464.2737 926.5328 926.5185 15.5 0 14 0.91 +1Score > 38 indicates identity
Score > 26 indicates homology		 INNLSIPR + Deamidated (NQ)
1054 479.2567 956.4988 956.5039 -5.32 0 14 0.91 +1Score > 40 indicates identity
Score > 26 indicates homology		 TLASSVHSR
3378 706.0623 2115.1651 2115.1398 12.0 1 14 0.5 +1Score > 34 indicates identity
Score > 24 indicates homology		 MPNTNPLFIDALASVVLKR + Deamidated (NQ); Oxidation (M)
2481 793.9313 1585.8480 1585.8311 10.7 1 14 0.69 +1Score > 39 indicates identity
Score > 25 indicates homology		 QAITNPNTIQSIKR + 3 Deamidated (NQ)
1210 510.7929 1019.5712 1019.5539 17.0 1 14 0.71 +1Score > 39 indicates identity
Score > 25 indicates homology		 QIELLYIE
1683 615.3329 1228.6512 1228.6411 8.22 0 14 0.68 +1Score > 41 indicates identity
Score > 25 indicates homology		 QQAADSVALVAR + Deamidated (NQ)
2759 860.4185 1718.8224 1718.8257 -1.91 1 14 0.31 +1Score > 40 indicates identity
Score > 21 indicates homology		 SGGIQGGISNGEIINMR + Deamidated (NQ); Oxidation (M)
1400 560.7911 1119.5676 1119.5780 -9.26 0 14 0.88 +1Score > 41 indicates identity
Score > 26 indicates homology		 QMIMILSPR + 2 Oxidation (M)
1315 537.8017 1073.5888 1073.5717 16.0 1 14 0.84 +1Score > 41 indicates identity
Score > 26 indicates homology		 TEVTQLIDR
2327 758.3730 1514.7314 1514.7253 4.07 0 14 0.32 +1Score > 41 indicates identity
Score > 22 indicates homology		 NTFSATSPPLLTHE + Deamidated (NQ)
2334 759.9385 1517.8624 1517.8705 -5.28 1 14 0.46 +1Score > 35 indicates identity
Score > 23 indicates homology		 ISLVNIKYLVLDE
1703 617.3163 1232.6180 1232.6149 2.52 1 14 0.76 +1Score > 42 indicates identity
Score > 25 indicates homology		 IQGTAEGAPFSR
3460 1078.0326 2154.0506 2154.0415 4.22 0 14 0.27 +1Score > 39 indicates identity
Score > 21 indicates homology		 VSMAVLTYPDGNYGNLVDAR
3572 1105.1351 2208.2556 2208.2531 1.15 1 14 0.31 +1Score > 31 indicates identity
Score > 21 indicates homology		 NIINLEPVLNVLRWHLHK + Deamidated (NQ)
3714 1154.0546 2306.0946 2306.0962 -0.69 1 14 0.13 +1Score > 39 indicates identity
Score > 18 indicates homology		 MNNLIKAYAAGVMSAAFLFGSE + 2 Deamidated (NQ)
3405 1064.5625 2127.1104 2127.0749 16.7 1 14 0.66 +1Score > 38 indicates identity
Score > 25 indicates homology		 IHSTFTIQVEYGTGPHAIR + Deamidated (NQ)
1067 480.7920 959.5694 959.5651 4.51 0 14 0.96 +1Score > 36 indicates identity
Score > 26 indicates homology		 KPLTSSLSK
2506 533.6329 1597.8769 1597.8675 5.84 0 14 0.26 +1Score > 38 indicates identity
Score > 21 indicates homology		 TVADLGSGNGSPGIILK
3719 770.0417 2307.1033 2307.1304 -11.8 1 14 0.069 +1Score > 39 indicates identity
Score > 15 indicates homology		 FQSDLKIINDCLDGLIQNAK + 3 Deamidated (NQ)
2708 565.9894 1694.9464 1694.9315 8.75 1 14 0.12 +1Score > 36 indicates identity
Score > 17 indicates homology		 GVRQGTQVLIAGGITPE
998 470.3026 938.5906 938.5913 -0.67 0 14 0.99 +1Score > 32 indicates identity
Score > 26 indicates homology		 LLAPAVLSR
270 586.3881 585.3808 585.3737 12.1 0 14 0.27 +1Score > 39 indicates identity
Score > 21 indicates homology		 ILDIL
2600 545.9695 1634.8867 1634.8628 14.6 1 14 0.16 +1Score > 37 indicates identity
Score > 18 indicates homology		 SLHLDPSDILELGAR
3336 1047.5364 2093.0582 2093.0252 15.8 0 14 0.065 +1Score > 39 indicates identity
Score > 14 indicates homology		 MSDAVPQVGSQFGPYQLLR + Deamidated (NQ)
1416 375.8658 1124.5756 1124.5536 19.6 1 14 0.97 +1Score > 40 indicates identity
Score > 26 indicates homology		 SALKYMGLNE
3421 1068.5618 2135.1090 2135.0858 10.9 1 14 0.69 +1Score > 38 indicates identity
Score > 25 indicates homology		 ILLSLDNQDLEINQVNHR + 2 Deamidated (NQ)
1248 519.2740 1036.5334 1036.5488 -14.8 1 14 0.63 +1Score > 40 indicates identity
Score > 24 indicates homology		 LAAAFKMNR + Oxidation (M)
874 441.2469 880.4792 880.4767 2.95 1 14 0.76 +1Score > 38 indicates identity
Score > 25 indicates homology		 QVHKLGAE
1342 363.1978 1086.5716 1086.5669 4.29 1 14 0.89 +1Score > 42 indicates identity
Score > 26 indicates homology		 SDRPELTAAK
1827 321.9276 1283.6813 1283.6946 -10.3 1 14 0.33 +1Score > 40 indicates identity
Score > 21 indicates homology		 ANARLDDVALAR
3467 1081.5742 2161.1338 2161.1630 -13.5 1 14 0.57 +1Score > 38 indicates identity
Score > 24 indicates homology		 SILGGFVINVNNKVIDTSIR + 3 Deamidated (NQ)
1604 600.3406 1198.6666 1198.6782 -9.64 1 14 0.99 +1Score > 40 indicates identity
Score > 26 indicates homology		 VDLLARGAATGR
1791 637.3329 1272.6512 1272.6310 15.9 1 14 0.41 +1Score > 41 indicates identity
Score > 22 indicates homology		 QTQQLQQELR + 2 Deamidated (NQ)
2197 366.4664 1461.8365 1461.8113 17.3 1 14 0.21 +1Score > 35 indicates identity
Score > 20 indicates homology		 MTVLTQEAIITVK + Oxidation (M)
1062 479.8026 957.5906 957.5971 -6.73 1 14 0.69 +1Score > 35 indicates identity
Score > 25 indicates homology		 LLLRTTNK
1658 610.8374 1219.6602 1219.6448 12.6 1 14 0.84 +1Score > 40 indicates identity
Score > 25 indicates homology		 GQSGQFLLELK + Deamidated (NQ)
2623 550.6290 1648.8652 1648.8533 7.23 1 14 0.44 +1Score > 39 indicates identity
Score > 23 indicates homology		 LAELAQLQDQLDHR
1464 571.8619 1141.7092 1141.7070 1.93 0 14 0.64 +1Score > 30 indicates identity
Score > 24 indicates homology		 ISIQVLQTLK
798 425.7270 849.4394 849.4266 15.1 0 14 0.78 +1Score > 42 indicates identity
Score > 25 indicates homology		 ITVGSDMK
2109 471.5990 1411.7752 1411.7670 5.76 1 14 0.61 +1Score > 38 indicates identity
Score > 24 indicates homology		 SVAALPINDNIKR + 2 Deamidated (NQ)
350 315.7010 629.3874 629.3860 2.23 0 14 0.98 +1Score > 42 indicates identity
Score > 26 indicates homology		 LATAVR
531 361.2168 720.4190 720.4170 2.81 0 14 0.96 +1Score > 38 indicates identity
Score > 26 indicates homology		 AGSIFVK
3970 509.6456 2543.1916 2543.1883 1.29 1 14 0.28 +1Score > 38 indicates identity
Score > 21 indicates homology		 LLNQMDGFDQTTNVKVIMATNR + 3 Deamidated (NQ); 2 Oxidation (M)
598 380.2353 758.4560 758.4538 2.99 0 14 0.86 +1Score > 40 indicates identity
Score > 26 indicates homology		 TALVINK + Deamidated (NQ)
2161 481.2461 1440.7165 1440.7222 -3.99 1 14 0.2 +1Score > 40 indicates identity
Score > 19 indicates homology		 QDHRVQDFLQR
2463 527.3038 1578.8896 1578.9093 -12.5 1 14 0.76 +1Score > 35 indicates identity
Score > 25 indicates homology		 VAEGGPNTLLLQVLR
3024 936.4662 1870.9178 1870.9326 -7.87 1 14 0.93 +1Score > 40 indicates identity
Score > 26 indicates homology		 GTKWIANNWINVDPSR + Deamidated (NQ)
315 307.6793 613.3440 613.3548 -17.5 0 14 0.54 +1Score > 37 indicates identity
Score > 24 indicates homology		 AGPLTR
912 449.7877 897.5608 897.5647 -4.34 0 14 0.35 +1Score > 36 indicates identity
Score > 22 indicates homology		 LTPLLSVR
3083 638.9906 1913.9500 1913.9516 -0.87 0 14 0.49 +1Score > 40 indicates identity
Score > 23 indicates homology		 LLGDDNLAGQADQAAMLAK
3180 663.3195 1986.9367 1986.9534 -8.43 1 14 0.29 +1Score > 39 indicates identity
Score > 21 indicates homology		 FSHINQPVKTLSGGNQQK + 5 Deamidated (NQ)
1116 491.7536 981.4926 981.4880 4.78 0 14 0.78 +1Score > 40 indicates identity
Score > 25 indicates homology		 GFLSSSQTR
1195 1015.6133 1014.6060 1014.5896 16.2 0 14 0.35 +1Score > 37 indicates identity
Score > 22 indicates homology		 SVIMALIPR + Oxidation (M)
686 398.1993 794.3840 794.3922 -10.3 0 14 0.32 +1Score > 41 indicates identity
Score > 21 indicates homology		 LHQPGNK + 2 Deamidated (NQ)
769 421.7230 841.4314 841.4294 2.49 1 14 0.74 +1Score > 39 indicates identity
Score > 25 indicates homology		 AGALEPER
2417 781.8846 1561.7546 1561.7770 -14.3 0 14 0.2 +1Score > 41 indicates identity
Score > 19 indicates homology		 GQNVGLIASLTTCAR + 2 Deamidated (NQ)
2692 559.2831 1674.8275 1674.8134 8.39 1 14 0.5 +1Score > 41 indicates identity
Score > 23 indicates homology		 GENIINIITGPNMSGK + 2 Deamidated (NQ); Oxidation (M)
2301 755.9493 1509.8840 1509.8654 12.3 0 14 0.19 +1Score > 33 indicates identity
Score > 19 indicates homology		 LIVLTDTPGILLNE
2381 774.8884 1547.7622 1547.7831 -13.5 1 14 0.32 +1Score > 41 indicates identity
Score > 21 indicates homology		 NVVEVTFNDLAAQK + Deamidated (NQ)
4453 795.6874 3178.7205 3178.6752 14.2 0 14 0.079 +1Score > 32 indicates identity
Score > 15 indicates homology		 SLVSFFCFFVISSCAAFISVPLLILWR
1268 524.2662 1046.5178 1046.4992 17.8 1 14 0.85 +1Score > 42 indicates identity
Score > 25 indicates homology		 TQGALGTENR + Deamidated (NQ)
2539 806.9540 1611.8934 1611.8944 -0.61 0 14 0.26 +1Score > 37 indicates identity
Score > 20 indicates homology		 DGGISGTVIAIAGVLNR
3019 623.3433 1867.0081 1867.0237 -8.37 0 14 0.46 +1Score > 37 indicates identity
Score > 23 indicates homology		 TIATLLNANVTPCLPLR + Deamidated (NQ)
799 426.2120 850.4094 850.4185 -10.6 1 14 0.74 +1Score > 42 indicates identity
Score > 25 indicates homology		 IEQFASR + Deamidated (NQ)
958 461.2577 920.5008 920.5113 -11.4 1 13 0.83 +1Score > 41 indicates identity
Score > 25 indicates homology		 IAVTSKMR + Oxidation (M)
2000 683.8411 1365.6676 1365.6524 11.2 1 13 0.43 +1Score > 41 indicates identity
Score > 22 indicates homology		 TANATALGYEQAR + Deamidated (NQ)
2280 500.5883 1498.7431 1498.7627 -13.1 1 13 0.9 +1Score > 40 indicates identity
Score > 26 indicates homology		 VANGQVVAELIDDR + Deamidated (NQ)
2491 531.2812 1590.8218 1590.8478 -16.3 0 13 0.85 +1Score > 40 indicates identity
Score > 25 indicates homology		 ISQGNHLPQSILQR + Deamidated (NQ)
3359 1056.0519 2110.0892 2110.0946 -2.55 1 13 0.19 +1Score > 38 indicates identity
Score > 19 indicates homology		 AGQDIQEVIDNLPINVPFK + Deamidated (NQ)
843 434.2939 866.5732 866.5701 3.58 0 13 0.16 +1Score > 29 indicates identity
Score > 18 indicates homology		 LGLKPLAR
616 382.2099 762.4052 762.3946 14.0 1 13 0.95 +1Score > 43 indicates identity
Score > 26 indicates homology		 MIEAVGK + Oxidation (M)
997 313.8357 938.4853 938.4934 -8.62 1 13 0.94 +1Score > 39 indicates identity
Score > 26 indicates homology		 ITHNEGLR
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