MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 3786)

Page: 1 2 3 4 5 6 … 38 Next 

Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
609 761.4052 760.3979 760.3967 1.67 1 41 0.096 +1Score > 43 indicates identity AQTEALK + Deamidated (NQ)
821 429.7612 857.5078 857.4971 12.6 1 40 0.059 +1Score > 41 indicates identity
Score > 41 indicates homology		 GAELVLTR
605 380.7206 759.4266 759.4239 3.65 0 40 0.09 +1Score > 42 indicates identity LNSALSR
562 367.6870 733.3594 733.3606 -1.59 1 40 0.064 +1Score > 42 indicates identity
Score > 40 indicates homology		 STIQER + Deamidated (NQ)
1178 505.7322 1009.4498 1009.4464 3.37 1 39 0.067 +1Score > 40 indicates identity QYANDRNK + 2 Deamidated (NQ)
372 643.4160 642.4087 642.4064 3.57 0 37 0.14 +1Score > 41 indicates identity AAAAVIK
261 572.3786 571.3713 571.3693 3.50 0 37 0.086 +1Score > 40 indicates identity
Score > 39 indicates homology		 AAAVIK
757 417.7467 833.4788 833.4759 3.51 0 37 0.068 +1Score > 39 indicates identity
Score > 38 indicates homology		 ITNAFLR
437 675.3699 674.3626 674.3599 4.07 1 37 0.21 +1Score > 43 indicates identity EAASVAK
287 601.3690 600.3617 600.3707 -15.0 1 37 0.27 -1Score > 44 indicates identity AGKVAR
865 879.4959 878.4886 878.4861 2.82 1 37 0.088 +1Score > 39 indicates identity KAFSDAIK
868 440.2532 878.4918 878.4861 6.51 0 37 0.085 +1Score > 38 indicates identity YILNSIR + Deamidated (NQ)
412 671.3743 670.3670 670.3802 -19.7 0 37 0.069 +1Score > 37 indicates identity WGALPK
285 601.3683 600.3610 600.3707 -16.2 1 37 0.29 +1Score > 44 indicates identity AGRVAK
1160 500.7761 999.5376 999.5349 2.75 0 36 0.1 +1Score > 40 indicates identity
Score > 39 indicates homology		 VANLGNLGDK
390 329.7112 657.4078 657.4061 2.64 0 36 0.17 +1Score > 41 indicates identity IDVALK
534 361.6782 721.3418 721.3548 -17.9 0 36 0.13 +1Score > 42 indicates identity
Score > 40 indicates homology		 YPTWR
1059 479.2993 956.5840 956.5728 11.7 1 36 0.11 +1Score > 39 indicates identity KMLPNLLK + Deamidated (NQ)
494 351.7062 701.3978 701.3959 2.71 1 35 0.35 +1Score > 43 indicates identity VEVQVK + Deamidated (NQ)
257 565.3360 564.3287 564.3271 2.79 0 35 0.07 +1Score > 38 indicates identity
Score > 36 indicates homology		 TVFAK
837 433.7469 865.4792 865.4770 2.63 0 35 0.11 +1Score > 38 indicates identity ALHQLQR + Deamidated (NQ)
423 673.3971 672.3898 672.3806 13.7 1 35 0.37 +1Score > 43 indicates identity AGELGVK
1049 478.7829 955.5512 955.5451 6.48 1 35 0.13 +1Score > 40 indicates identity
Score > 38 indicates homology		 VAEQLAAVR
727 408.2358 814.4570 814.4548 2.70 1 35 0.38 +1Score > 43 indicates identity EIIIDGR
642 387.2124 772.4102 772.4079 3.04 1 34 0.44 +1Score > 43 indicates identity IQVQER + Deamidated (NQ)
244 544.3484 543.3411 543.3493 -15.0 1 34 0.48 +1Score > 44 indicates identity KVAAR
373 643.4171 642.4098 642.4064 5.28 1 34 0.28 +1Score > 41 indicates identity AIKLAQ
788 848.3918 847.3845 847.3937 -10.8 1 34 0.13 +1Score > 41 indicates identity
Score > 38 indicates homology		 DDTWRR
1055 479.2732 956.5318 956.5291 2.87 1 34 0.2 +1Score > 41 indicates identity
Score > 40 indicates homology		 LGDVVEAVR
725 407.7536 813.4926 813.5072 -17.9 0 34 0.18 +1Score > 41 indicates identity
Score > 39 indicates homology		 NIILVSR
688 398.2228 794.4310 794.4286 3.04 0 34 0.2 +1Score > 39 indicates identity AVSSYLR
1767 631.3560 1260.6974 1260.6965 0.73 0 33 0.21 +1Score > 39 indicates identity QFILSQVALLE + Deamidated (NQ)
1432 566.2872 1130.5598 1130.5567 2.74 1 33 0.11 +1Score > 42 indicates identity
Score > 36 indicates homology		 VVELNASDQR + Deamidated (NQ)
431 673.4283 672.4210 672.4170 5.95 0 33 0.26 +1Score > 40 indicates identity AVSVAVK
576 745.4591 744.4518 744.4494 3.30 0 33 0.36 +1Score > 41 indicates identity ITAALTR
767 841.5173 840.5100 840.5069 3.74 1 33 0.099 +1Score > 35 indicates identity KIDQIPK
1357 549.3154 1096.6162 1096.6063 9.09 1 33 0.16 +1Score > 37 indicates identity LPDIGPKMAR
552 365.2302 728.4458 728.4432 3.60 1 32 0.24 +1Score > 42 indicates identity
Score > 39 indicates homology		 VAIEAVK
1165 502.2581 1002.5016 1002.4982 3.47 0 32 0.52 +1Score > 42 indicates identity IANISGDGQK + Deamidated (NQ)
783 423.2298 844.4450 844.4290 19.0 1 32 0.11 +1Score > 43 indicates identity
Score > 35 indicates homology		 LQAAEDAK
1861 651.3586 1300.7026 1300.6888 10.7 1 31 0.15 +1Score > 41 indicates identity
Score > 36 indicates homology		 ANIWLVRGSQR + 2 Deamidated (NQ)
1279 528.7957 1055.5768 1055.5723 4.28 1 31 0.41 +1Score > 40 indicates identity LQAQAQRLE
434 338.1872 674.3598 674.3711 -16.7 0 31 0.19 +1Score > 43 indicates identity
Score > 37 indicates homology		 TIGSAAR
1678 614.8378 1227.6610 1227.6823 -17.3 0 31 0.42 +1Score > 40 indicates identity IDVGGSAAVLGAAK
1232 516.2657 1030.5168 1030.5043 12.2 0 31 0.24 +1Score > 42 indicates identity
Score > 37 indicates homology		 LTSNEPSQR
690 796.4239 795.4166 795.4014 19.1 0 31 0.33 +1Score > 39 indicates identity
Score > 39 indicates homology		 SALTYIE
1230 516.2607 1030.5068 1030.5043 2.46 0 31 0.093 +1Score > 41 indicates identity
Score > 33 indicates homology		 LTSNEPSQR
789 424.6997 847.3848 847.3937 -10.4 1 31 0.1 +1Score > 41 indicates identity
Score > 33 indicates homology		 DDTWRR
391 329.7112 657.4078 657.4061 2.64 0 31 0.19 +1Score > 41 indicates identity
Score > 36 indicates homology		 IDVALK
1242 1035.5885 1034.5812 1034.6012 -19.3 1 31 0.23 +1Score > 38 indicates identity
Score > 37 indicates homology		 IEIYIVGTK
1172 505.2524 1008.4902 1008.4876 2.63 1 31 0.62 +1Score > 41 indicates identity EGAQAYLTR + Deamidated (NQ)
748 415.7417 829.4688 829.4545 17.3 1 31 0.51 +1Score > 43 indicates identity
Score > 40 indicates homology		 KIIDNVE
791 424.7003 847.3860 847.3937 -8.98 1 31 0.079 +1Score > 41 indicates identity
Score > 32 indicates homology		 DDTWRR
885 444.2519 886.4892 886.4872 2.30 1 31 0.75 +1Score > 42 indicates identity
Score > 42 indicates homology		 DGLALRDK
1007 471.7736 941.5326 941.5406 -8.48 1 31 0.41 +1Score > 39 indicates identity ARAGAQVIR + Deamidated (NQ)
396 331.6972 661.3798 661.3693 15.9 1 30 0.36 +1Score > 38 indicates identity ISMRR
553 365.2358 728.4570 728.4545 3.56 0 30 0.43 +1Score > 42 indicates identity
Score > 39 indicates homology		 IAVSAIR
857 437.7516 873.4886 873.4920 -3.79 0 30 0.39 +1Score > 42 indicates identity
Score > 39 indicates homology		 AQITTLAR + Deamidated (NQ)
633 386.2227 770.4308 770.4286 2.87 0 30 0.14 +1Score > 39 indicates identity
Score > 34 indicates homology		 LGQSLPR + Deamidated (NQ)
1851 648.8708 1295.7270 1295.7193 6.02 1 30 0.26 +1Score > 37 indicates identity MSMLSTLLLKK + 2 Oxidation (M)
1797 637.8668 1273.7190 1273.7030 12.6 1 30 0.22 +1Score > 38 indicates identity
Score > 36 indicates homology		 SAGEFIAPLTLR
1044 476.2541 950.4936 950.5073 -14.3 1 30 0.58 +1Score > 40 indicates identity ISNFTEIK
395 330.6878 659.3610 659.3715 -15.8 1 30 0.81 +1Score > 45 indicates identity
Score > 42 indicates homology		 NKVGSR
222 516.2924 515.2851 515.2816 6.90 0 30 0.48 +1Score > 40 indicates identity
Score > 39 indicates homology		 AGAAAR
1063 480.2540 958.4934 958.4971 -3.83 0 30 0.64 +1Score > 43 indicates identity
Score > 41 indicates homology		 LIAVSNDID
1231 516.2610 1030.5074 1030.4931 13.9 0 30 0.45 +1Score > 41 indicates identity
Score > 39 indicates homology		 LTQNLAGGQE + Deamidated (NQ)
1764 629.8565 1257.6984 1257.6816 13.4 0 30 0.5 +1Score > 39 indicates identity LVIAVANDSNIK + 2 Deamidated (NQ)
1163 501.2868 1000.5590 1000.5553 3.76 1 30 0.4 +1Score > 41 indicates identity
Score > 38 indicates homology		 QATLEQALK
913 450.2874 898.5602 898.5488 12.8 0 30 0.2 +1Score > 37 indicates identity
Score > 35 indicates homology		 AVLDQLLK
2014 457.5966 1369.7680 1369.7677 0.20 1 30 0.077 +1Score > 38 indicates identity
Score > 31 indicates homology		 NARLSILNAQLR + 2 Deamidated (NQ)
559 366.7046 731.3946 731.3813 18.2 1 30 0.16 +1Score > 44 indicates identity
Score > 34 indicates homology		 TGENALK
480 350.7222 699.4298 699.4391 -13.3 1 30 0.81 +1Score > 41 indicates identity AAKAAIR
750 416.2468 830.4790 830.4749 4.98 1 30 0.62 +1Score > 40 indicates identity TQVELLK + Deamidated (NQ)
1120 492.8131 983.6116 983.6015 10.3 0 30 0.13 +1Score > 35 indicates identity
Score > 33 indicates homology		 QLAILADLK
1420 564.8232 1127.6318 1127.6299 1.76 0 29 0.15 +1Score > 39 indicates identity
Score > 34 indicates homology		 LQGQNVLTVR + Deamidated (NQ)
601 380.2540 758.4934 758.4902 4.32 0 29 0.062 +1Score > 30 indicates identity VISILSK
516 357.2517 712.4888 712.4847 5.85 0 29 0.17 +1Score > 34 indicates identity NIIILK
389 329.7103 657.4060 657.4061 -0.099 1 29 0.073 +1Score > 41 indicates identity
Score > 30 indicates homology		 IEIVGK
243 544.3465 543.3392 543.3380 2.17 0 29 0.76 +1Score > 44 indicates identity
Score > 41 indicates homology		 IAGGVK
387 328.6909 655.3672 655.3765 -14.2 0 29 0.18 +1Score > 37 indicates identity
Score > 34 indicates homology		 IDRPR
1768 631.3562 1260.6978 1260.6747 18.3 1 29 0.11 +1Score > 39 indicates identity
Score > 32 indicates homology		 MKLLNQLIDR + 2 Deamidated (NQ); Oxidation (M)
2023 688.8732 1375.7318 1375.7170 10.8 0 29 0.066 +1Score > 40 indicates identity
Score > 30 indicates homology		 DIMFGPIALGQSK
1680 614.8380 1227.6614 1227.6823 -17.0 0 29 0.48 +1Score > 40 indicates identity
Score > 38 indicates homology		 IDVGGSAAVLGAAK
560 366.7048 731.3950 731.3926 3.33 0 29 0.32 +1Score > 44 indicates identity
Score > 36 indicates homology		 TGTGQIR
2097 470.5633 1408.6681 1408.6616 4.58 1 29 0.061 +1Score > 41 indicates identity
Score > 29 indicates homology		 SASGDNMSKIIDR + Oxidation (M)
2411 520.9501 1559.8285 1559.8195 5.75 1 29 0.19 +1Score > 40 indicates identity
Score > 34 indicates homology		 LDAGFLNTIPETLR + Deamidated (NQ)
755 417.2501 832.4856 832.4840 1.93 0 29 0.38 +1Score > 38 indicates identity
Score > 37 indicates homology		 ITLAMLR + Oxidation (M)
866 440.2520 878.4894 878.4861 3.76 1 29 0.5 +1Score > 39 indicates identity
Score > 38 indicates homology		 KAFSDAIK
472 348.1722 694.3298 694.3286 1.82 1 29 0.67 +1Score > 41 indicates identity
Score > 40 indicates homology		 IDEYR
2333 506.9550 1517.8432 1517.8202 15.2 1 29 0.12 +1Score > 37 indicates identity
Score > 32 indicates homology		 VKFNVINTAVQGAR + 2 Deamidated (NQ)
888 444.7430 887.4714 887.4712 0.25 0 29 0.21 +1Score > 43 indicates identity
Score > 34 indicates homology		 NNDTLIAK
689 398.7155 795.4164 795.4014 18.9 0 29 0.12 +1Score > 39 indicates identity
Score > 32 indicates homology		 AVTTYLE
2398 778.4473 1554.8800 1554.8869 -4.39 1 29 0.34 +1Score > 36 indicates identity
Score > 36 indicates homology		 DKIIVQSVPDVTLK + Deamidated (NQ)
1313 536.7875 1071.5604 1071.5672 -6.33 1 28 0.33 +1Score > 42 indicates identity
Score > 36 indicates homology		 VQAAAQRQAK + 2 Deamidated (NQ)
1191 508.2629 1014.5112 1014.5094 1.82 1 28 0.91 +1Score > 41 indicates identity IADGLNAGER
923 452.2444 902.4742 902.4821 -8.74 0 28 0.45 +1Score > 43 indicates identity
Score > 38 indicates homology		 ISAADVATR
1986 453.5818 1357.7236 1357.7201 2.55 1 28 0.9 +1Score > 40 indicates identity
Score > 40 indicates homology		 QTALSIEQQLAR + Deamidated (NQ)
2066 699.8899 1397.7652 1397.7514 9.90 1 28 0.21 +1Score > 39 indicates identity
Score > 34 indicates homology		 VLALLNDNLQRE + Deamidated (NQ)
1285 529.7936 1057.5726 1057.5590 12.9 0 28 0.97 +1Score > 42 indicates identity
Score > 41 indicates homology		 IAVSPMLGNR + Deamidated (NQ)
995 469.7332 937.4518 937.4505 1.42 1 28 0.063 +1Score > 41 indicates identity
Score > 29 indicates homology		 VFDSSVER
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