MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita6 sp |
| MS data file | : | PRT1270_T-BRSC_6_20250714122635.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:38:56 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,869 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU2_PSEPK | 1352 | Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1 | |
| 4 | EFTU_CYAP7 | 34 | Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1 | |
| 3 | EFTU_CARRP | 180 | Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1 | |
| 2 | EFTU1_PSEPK | 1236 | Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_PSEPG | 1121 | ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1 | |
| 2 | ATPB_PSEFS | 949 | ATP synthase subunit beta OS=Pseudomonas fluorescens (strain SBW25) GN=atpD PE=3 SV=1 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPA_PSEPK | 954 | ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1 | |
| 2 | ATPA_RICAH | 173 | ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1 | |
| 3 | ATPA_VEREI | 163 | ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1 | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_PSEPK | 667 | 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1 | |
| 4 | CH602_SORC5 | 69 | 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1 | |
| 3 | CH60_COLMA | 82 | 60 kDa chaperonin OS=Colwellia maris GN=groL PE=3 SV=2 | |
| 2 | CH601_ECOK1 | 119 | 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1 | |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOC_PSEPK | 451 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1 | |
| 4 | RPOC_PELUB | 108 | DNA-directed RNA polymerase subunit beta' OS=Pelagibacter ubique GN=rpoC PE=3 SV=1 | |
| 3 | RPOC_SYNAS | 149 | DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1 | |
| 2 | RPOB_PSEP1 | 316 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoB PE=3 SV=1 | |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL1_PSEE4 | 451 | 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OTCC_PSEPK | 445 | Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARCA_PSEPK | 405 | Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DLDH2_PSEPU | 387 | Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 9.1 | DLDH2_PSEPU |
387 | 50093 | 29 (12) | 15 (7) | 0.56 | |
| Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4 | |||||||
29 peptide matches (20 non-duplicate, 9 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 199 |
2 |
487.3253 | 486.3180 | 486.3166 | 2.98 | 0 | 31 | 0.16 | 1Score > 42 indicates identity Score > 36 indicates homology |
K.AGIVK.N | |
| 208 |
501.3410 | 500.3337 | 500.3322 | 3.02 | 0 | 26 | 0.18 | 1Score > 42 indicates identity Score > 31 indicates homology |
K.LLAGK.K | ||
| 321 |
308.2132 | 614.4118 | 614.4115 | 0.52 | 1 | 8 | 1.1 | 9Score > 41 indicates identity Score > 21 indicates homology |
R.KAGIVK.N | ||
| 326 |
308.6872 | 615.3598 | 615.3592 | 1.12 | 0 | 19 | 1.2 | 6Score > 46 indicates identity Score > 32 indicates homology |
K.VIADAK.T | ||
| 329 |
616.3685 | 615.3612 | 615.3592 | 3.35 | 0 | 33 | 0.55 | 1Score > 45 indicates identity Score > 43 indicates homology |
K.VIADAK.T | ||
| 336 |
1 |
312.1739 | 622.3332 | 622.3326 | 1.00 | 0 | 8 | 1.5 | 5Score > 41 indicates identity Score > 22 indicates homology |
K.ITFDK.L | |
| 460 |
689.3930 | 688.3857 | 688.3755 | 14.8 | 1 | 24 | 0.99 | 7Score > 43 indicates identity Score > 36 indicates homology |
K.TEQALK.A | ||
| 480 |
350.7222 | 699.4298 | 699.4279 | 2.78 | 0 | 26 | 2 | 5Score > 41 indicates identity |
K.AAQLGLK.T | ||
| 482 |
1 |
700.4379 | 699.4306 | 699.4279 | 3.89 | 0 | 37 | 0.15 | 1Score > 41 indicates identity |
K.AAQLGLK.T | |
| 542 |
2 |
364.2460 | 726.4774 | 726.4752 | 3.09 | 0 | 35 | 0.057 | 1Score > 36 indicates identity |
U | K.LIVAVGR.R |
| 949 |
1 |
460.2503 | 918.4860 | 918.4811 | 5.43 | 0 | 35 | 0.058 | 1Score > 42 indicates identity Score > 35 indicates homology |
U | K.ALLDSSWK.Y |
| 1407 |
1120.6428 | 1119.6355 | 1119.6288 | 5.99 | 0 | 80 | 3.5e-006 | 1Score > 38 indicates identity |
U | K.NLTGGVATLFK.A | |
| 1408 |
560.8273 | 1119.6400 | 1119.6288 | 10.0 | 0 | 51 | 0.0018 | 1Score > 36 indicates identity |
U | K.NLTGGVATLFK.A | |
| 1525 |
390.2084 | 1167.6034 | 1167.5996 | 3.20 | 0 | 34 | 0.0052 | 1Score > 40 indicates identity Score > 24 indicates homology |
U | K.ANGVTSIQGHGK.L | |
| 1526 |
584.8091 | 1167.6036 | 1167.5996 | 3.43 | 0 | 52 | 0.0014 | 1Score > 40 indicates identity Score > 36 indicates homology |
U | K.ANGVTSIQGHGK.L | |
| 1557 |
591.2872 | 1180.5598 | 1180.5547 | 4.40 | 0 | 38 | 0.006 | 1Score > 41 indicates identity Score > 28 indicates homology |
U | R.AMAANDTGGFVK.V | |
| 2796 |
866.9971 | 1731.9796 | 1731.9560 | 13.7 | 0 | 37 | 0.0011 | 1Score > 34 indicates identity Score > 20 indicates homology |
U | K.FDVVVIGAGPGGYVAAIK.A | |
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_6_20250714122635.rawScore > 29 indicates identity |
|||||||||||
| 3014 |
1 |
934.0565 | 1866.0984 | 1866.0727 | 13.8 | 1 | 81 | 3.4e-007 | 1Score > 29 indicates identity |
U | R.LGVIGAGVIGLELGSVWAR.L |
| -4.99 | 0 | 17 | 0.95 | 2 | IGIVQGVILSQQIVITAL + 2 Deamidated (NQ) | ||||||
| 5.97 | 1 | 11 | 3.7 | 3 | LRQGLTMLPQLLVNVR + Oxidation (M) | ||||||
| 3015 |
1 |
623.0406 | 1866.1000 | 1866.0727 | 14.6 | 1 | 77 | 1.9e-007 | 1Score > 29 indicates identity Score > 22 indicates homology |
U | R.LGVIGAGVIGLELGSVWAR.L |
| 3330 |
1046.0967 | 2090.1788 | 2090.1412 | 18.0 | 1 | 75 | 2.7e-006 | 1Score > 32 indicates identity |
U | M.TQKFDVVVIGAGPGGYVAAIK.A + Deamidated (NQ) | |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| DLDH2_PSEAE |
233 | 50362 | 9.1 | ||
| Dihydrolipoamide dehydrogenase OS=Pseudomonas aeruginosa GN=lpdG PE=3 SV=1 | |||||
| 2 samesets of DLDH2_PSEAE | |||||
| DLDH_AZOVI |
233 | 49707 | |||
| Dihydrolipoyl dehydrogenase OS=Azotobacter vinelandii PE=1 SV=1 | |||||
| DLDH_PSEFL |
233 | 50348 | |||
| Dihydrolipoyl dehydrogenase OS=Pseudomonas fluorescens GN=lpd PE=1 SV=3 | |||||
| DFX_METTH |
34 | 14264 | 9.1 | ||
| Desulfoferrodoxin homolog OS=Methanobacterium thermoautotrophicum GN=MTH_757 PE=3 SV=1 | |||||
| 6 samesets of DFX_METTH | |||||
| DLDH_THESC |
34 | 48589 | |||
| Dihydrolipoyl dehydrogenase OS=Thermus scotoductus GN=lpd PE=1 SV=2 | |||||
| DLDH3_HALMA |
34 | 50375 | |||
| Dihydrolipoyl dehydrogenase 3 OS=Haloarcula marismortui GN=lpdA3 PE=3 SV=1 | |||||
| GLMU_DECAR |
34 | 48067 | |||
| Bifunctional protein glmU OS=Dechloromonas aromatica (strain RCB) GN=glmU PE=3 SV=1 | |||||
| RPPH_ACICJ |
34 | 18342 | |||
| RNA pyrophosphohydrolase OS=Acidiphilium cryptum (strain JF-5) GN=rppH PE=3 SV=1 | |||||
| YNF1_RHOCA |
34 | 27985 | |||
| Uncharacterized 27.7 kDa protein in nifB 3'region OS=Rhodobacter capsulatus PE=4 SV=1 | |||||
| HSE1_SCHPO |
34 | 42699 | |||
| Class E vacuolar protein-sorting machinery protein hse1 OS=Schizosaccharomyces pombe GN=hse1 PE=2 SV=1 | |||||
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DBHB_PSEAE | 318 | DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3 |
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