MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 187)

Page: Previous 1 …4 5 6 7 8 9 10 11 12 13 14 … 19 Next 

+81

Accession Score Description
1 CLPX_CLOB8 59 ATP-dependent Clp protease ATP-binding subunit clpX OS=Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) GN=clpX PE=3 SV=1

+82

Accession Score Description
1 FLAE_VIBCH 59 Flagellin E OS=Vibrio cholerae GN=flaE PE=3 SV=1

+83

Accession Score Description
1 RS9_PSEE4 59 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1

+84

Accession Score Description
1 RS20_PSE14 58 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1

+85

Accession Score Description
1 RDRP_ACLSA 57 RNA-directed RNA polymerase OS=Apple chlorotic leaf spot virus (isolate apple) PE=4 SV=1

+86

Accession Score Description
1 TIG_PSEPK 55 Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2

+87

Accession Score Description
1 PUR5_PSEMY 55 Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas mendocina (strain ymp) GN=purM PE=3 SV=1

+88

Accession Score Description
1 MOAC_METB6 55 Probable molybdenum cofactor biosynthesis protein C OS=Methanoregula boonei (strain 6A8) GN=moaC PE=3 SV=1

-89

Accession Score Description
1 RS4_PSEPW 55 30S ribosomal protein S4 OS=Pseudomonas putida (strain W619) GN=rpsD PE=3 SV=1
Score Mass Matches Sequences emPAI
89.1 RS4_PSEPW 55 23287 5 (2) 3 (2) 0.21
30S ribosomal protein S4 OS=Pseudomonas putida (strain W619) GN=rpsD PE=3 SV=1
3 samesets of RS4_PSEPW
RS4_PSEP1 55 23270 4 (2) 2 (2) 0.21
30S ribosomal protein S4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsD PE=3 SV=1
RS4_PSEPG 55 23284 4 (2) 2 (2) 0.21
30S ribosomal protein S4 OS=Pseudomonas putida (strain GB-1) GN=rpsD PE=3 SV=1
RS4_PSEPK 55 23270 4 (2) 2 (2) 0.21
30S ribosomal protein S4 OS=Pseudomonas putida (strain KT2440) GN=rpsD PE=3 SV=1

-5 peptide matches (4 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
754   417.2201 832.4256 832.4402 -17.5 1 4 2.2 +9Score > 43 indicates identity
Score > 19 indicates homology		 U E.KSSNQLR.I + Deamidated (NQ)
1298   534.2615 1066.5084 1066.5043 3.86 0 48 0.0012 +1Score > 41 indicates identity
Score > 32 indicates homology		 U R.QSDYGTQLR.E
3151 +1 657.3729 1969.0969 1969.0745 11.3 0 16 0.14 +1Score > 34 indicates identity
Score > 20 indicates homology		 U K.TVNIPSYQVRPGDVVAVR.E
3154   986.0532 1970.0918 1970.0585 16.9 0 34 0.0047 +1Score > 35 indicates identity
Score > 23 indicates homology		 U K.TVNIPSYQVRPGDVVAVR.E + Deamidated (NQ)

1 subset or intersection (8 subset proteins in total)

Score Mass Subset of
RS4_PSE14 48 23290 89.1
30S ribosomal protein S4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsD PE=3 SV=1
7 samesets of RS4_PSE14
RS4_PSEF5 48 23289
30S ribosomal protein S4 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsD PE=3 SV=1
RS4_PSEE4 48 23361
30S ribosomal protein S4 OS=Pseudomonas entomophila (strain L48) GN=rpsD PE=3 SV=1
RS4_PSEFS 48 23290
30S ribosomal protein S4 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsD PE=3 SV=1
RS4_PSEMY 48 23217
30S ribosomal protein S4 OS=Pseudomonas mendocina (strain ymp) GN=rpsD PE=3 SV=1
RS4_PSEPF 48 23246
30S ribosomal protein S4 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsD PE=3 SV=1
RS4_PSESM 48 23290
30S ribosomal protein S4 OS=Pseudomonas syringae pv. tomato GN=rpsD PE=3 SV=1
RS4_PSEU2 48 23290
30S ribosomal protein S4 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsD PE=3 SV=1

+90

Accession Score Description
1 ISPD_ALKMQ 54 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase OS=Alkaliphilus metalliredigens (strain QYMF) GN=ispD PE=3 SV=1
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