MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 187)

Page: Previous 1 …4 5 6 7 8 9 10 11 12 13 14 … 19 Next 

+81

Accession Score Description
1 CLPX_CLOB8 59 ATP-dependent Clp protease ATP-binding subunit clpX OS=Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) GN=clpX PE=3 SV=1

+82

Accession Score Description
1 FLAE_VIBCH 59 Flagellin E OS=Vibrio cholerae GN=flaE PE=3 SV=1

+83

Accession Score Description
1 RS9_PSEE4 59 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1

+84

Accession Score Description
1 RS20_PSE14 58 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1

+85

Accession Score Description
1 RDRP_ACLSA 57 RNA-directed RNA polymerase OS=Apple chlorotic leaf spot virus (isolate apple) PE=4 SV=1

-86

Accession Score Description
1 TIG_PSEPK 55 Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2
Score Mass Matches Sequences emPAI
86.1 TIG_PSEPK 55 48487 4 (3) 3 (2) 0.10
Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2
5 samesets of TIG_PSEPK
TIG_PSEP1 55 48429 4 (3) 3 (2) 0.10
Trigger factor OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=tig PE=3 SV=1
TIG_PSEMY 55 48300 3 (3) 2 (2) 0.10
Trigger factor OS=Pseudomonas mendocina (strain ymp) GN=tig PE=3 SV=1
TIG_PSEPG 55 48501 3 (3) 2 (2) 0.10
Trigger factor OS=Pseudomonas putida (strain GB-1) GN=tig PE=3 SV=1
TIG_PSEPW 55 48456 3 (3) 2 (2) 0.10
Trigger factor OS=Pseudomonas putida (strain W619) GN=tig PE=3 SV=1
TIG_PSEE4 55 48359 3 (3) 2 (2) 0.10
Trigger factor OS=Pseudomonas entomophila (strain L48) GN=tig PE=3 SV=1

-4 peptide matches (3 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1102   486.7992 971.5838 971.5764 7.68 0 27 0.81 +1Score > 39 indicates identity K.GTLLVLGSGR.M
1515   582.3276 1162.6406 1162.6346 5.20 0 39 0.007 +1Score > 39 indicates identity
Score > 30 indicates homology		 U K.LNPAGAPAVEPK.S
1718 +1 619.9152 1237.8158 1237.8010 12.0 1 38 0.00084 +1Score > 19 indicates identity U R.VVLGLIVAEVVK.Q

2 subsets and intersections (11 subset proteins in total)

Score Mass Subset of
TIG_PSEA7 43 48518 86.1
Trigger factor OS=Pseudomonas aeruginosa (strain PA7) GN=tig PE=3 SV=1
7 samesets of TIG_PSEA7
TIG_PSEU5 43 48281
Trigger factor OS=Pseudomonas stutzeri (strain A1501) GN=tig PE=3 SV=1
TIG_PSEAB 43 48552
Trigger factor OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=tig PE=3 SV=1
TIG_PSEAE 43 48552
Trigger factor OS=Pseudomonas aeruginosa GN=tig PE=3 SV=1
TIG_PSEA8 43 48552
Trigger factor OS=Pseudomonas aeruginosa (strain LESB58) GN=tig PE=3 SV=1
TIG_PSEFS 43 48316
Trigger factor OS=Pseudomonas fluorescens (strain SBW25) GN=tig PE=3 SV=1
TIG_PSEPF 43 48455
Trigger factor OS=Pseudomonas fluorescens (strain Pf0-1) GN=tig PE=3 SV=1
TIG_PSEF5 43 48539
Trigger factor OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=tig PE=3 SV=1
TIG_PSE14 39 48654 86.1
Trigger factor OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=tig PE=3 SV=1
2 samesets of TIG_PSE14
TIG_PSESM 39 48616
Trigger factor OS=Pseudomonas syringae pv. tomato GN=tig PE=3 SV=1
TIG_PSEU2 39 48644
Trigger factor OS=Pseudomonas syringae pv. syringae (strain B728a) GN=tig PE=3 SV=1

+87

Accession Score Description
1 PUR5_PSEMY 55 Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas mendocina (strain ymp) GN=purM PE=3 SV=1

+88

Accession Score Description
1 MOAC_METB6 55 Probable molybdenum cofactor biosynthesis protein C OS=Methanoregula boonei (strain 6A8) GN=moaC PE=3 SV=1

+89

Accession Score Description
1 RS4_PSEPW 55 30S ribosomal protein S4 OS=Pseudomonas putida (strain W619) GN=rpsD PE=3 SV=1

+90

Accession Score Description
1 ISPD_ALKMQ 54 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase OS=Alkaliphilus metalliredigens (strain QYMF) GN=ispD PE=3 SV=1
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