MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 187)

Page: Previous 1 …4 5 6 7 8 9 10 11 12 13 14 … 19 Next 

+81

Accession Score Description
1 CLPX_CLOB8 59 ATP-dependent Clp protease ATP-binding subunit clpX OS=Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) GN=clpX PE=3 SV=1

+82

Accession Score Description
1 FLAE_VIBCH 59 Flagellin E OS=Vibrio cholerae GN=flaE PE=3 SV=1

+83

Accession Score Description
1 RS9_PSEE4 59 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1

+84

Accession Score Description
1 RS20_PSE14 58 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1

-85

Accession Score Description
1 RDRP_ACLSA 57 RNA-directed RNA polymerase OS=Apple chlorotic leaf spot virus (isolate apple) PE=4 SV=1
Score Mass Matches Sequences emPAI
85.1 RDRP_ACLSA 57 219105 6 (3) 2 (1) 0.01
RNA-directed RNA polymerase OS=Apple chlorotic leaf spot virus (isolate apple) PE=4 SV=1
15 samesets of RDRP_ACLSA
RPOC2_PINTH 57 140153 6 (3) 2 (1) 0.02
DNA-directed RNA polymerase subunit beta'' OS=Pinus thunbergii GN=rpoC2 PE=3 SV=2
NFRA_STAAB 57 28605 5 (3) 1 (1) 0.08
NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain bovine RF122 / ET3-1) GN=nfrA PE=3 SV=1
NFRA_STAAC 57 28579 5 (3) 1 (1) 0.08
NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain COL) GN=nfrA PE=3 SV=1
NFRA_STAAM 57 28591 5 (3) 1 (1) 0.08
NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) GN=nfrA PE=1 SV=1
NFRA_STAAN 57 28591 5 (3) 1 (1) 0.08
NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain N315) GN=nfrA PE=3 SV=1
NFRA_STAAR 57 28534 5 (3) 1 (1) 0.08
NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain MRSA252) GN=nfrA PE=3 SV=1
NFRA_STAAS 57 28578 5 (3) 1 (1) 0.08
NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain MSSA476) GN=nfrA PE=3 SV=1
NFRA_STAAW 57 28578 5 (3) 1 (1) 0.08
NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain MW2) GN=nfrA PE=3 SV=1
RDRP_ACLSP 57 219160 5 (3) 1 (1) 0.01
RNA-directed RNA polymerase OS=Apple chlorotic leaf spot virus (isolate plum P863) PE=4 SV=1
DNM3L_HUMAN 57 44611 5 (3) 1 (1) 0.05
DNA (cytosine-5)-methyltransferase 3-like OS=Homo sapiens GN=DNMT3L PE=1 SV=2
DNM3L_MOUSE 57 49159 5 (3) 1 (1) 0.05
DNA (cytosine-5)-methyltransferase 3-like OS=Mus musculus GN=Dnmt3l PE=1 SV=1
DNM3L_RAT 57 49388 5 (3) 1 (1) 0.05
DNA (cytosine-5)-methyltransferase 3-like OS=Rattus norvegicus GN=Dnmt3l PE=2 SV=1
NFRA_STAA3 57 28579 5 (3) 1 (1) 0.08
NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain USA300) GN=nfrA PE=3 SV=1
NRFA_STAA8 57 28579 5 (3) 1 (1) 0.08
NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain NCTC 8325) GN=nfrA PE=1 SV=1
UN84B_MOUSE 57 78318 5 (3) 1 (1) 0.03
Protein unc-84 homolog B OS=Mus musculus GN=Unc84b PE=1 SV=2

-6 peptide matches (3 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
426 +1 673.4005 672.3932 672.3919 2.03 0 23 1.5 +6Score > 43 indicates identity
Score > 38 indicates homology		 U K.SGLLQR.M
428 +2 337.2045 672.3944 672.3919 3.85 0 48 0.0097 +1Score > 41 indicates identity U K.SGLLQR.M
822   430.2148 858.4150 858.3979 19.9 0 8 1.3 +2Score > 40 indicates identity
Score > 21 indicates homology		 U R.SFMSMIK.G + Oxidation (M)

+86

Accession Score Description
1 TIG_PSEPK 55 Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2

+87

Accession Score Description
1 PUR5_PSEMY 55 Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas mendocina (strain ymp) GN=purM PE=3 SV=1

+88

Accession Score Description
1 MOAC_METB6 55 Probable molybdenum cofactor biosynthesis protein C OS=Methanoregula boonei (strain 6A8) GN=moaC PE=3 SV=1

+89

Accession Score Description
1 RS4_PSEPW 55 30S ribosomal protein S4 OS=Pseudomonas putida (strain W619) GN=rpsD PE=3 SV=1

+90

Accession Score Description
1 ISPD_ALKMQ 54 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase OS=Alkaliphilus metalliredigens (strain QYMF) GN=ispD PE=3 SV=1
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