MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 187)

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+81

Accession Score Description
1 CLPX_CLOB8 59 ATP-dependent Clp protease ATP-binding subunit clpX OS=Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) GN=clpX PE=3 SV=1

+82

Accession Score Description
1 FLAE_VIBCH 59 Flagellin E OS=Vibrio cholerae GN=flaE PE=3 SV=1

+83

Accession Score Description
1 RS9_PSEE4 59 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1

-84

Accession Score Description
1 RS20_PSE14 58 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1
Score Mass Matches Sequences emPAI
84.1 RS20_PSE14 58 10006 2 (1) 1 (1) 0.24
30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1
10 samesets of RS20_PSE14
RS20_PSEE4 58 10063 2 (1) 1 (1) 0.24
30S ribosomal protein S20 OS=Pseudomonas entomophila (strain L48) GN=rpsT PE=3 SV=1
RS20_PSEF5 58 10019 2 (1) 1 (1) 0.24
30S ribosomal protein S20 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsT PE=3 SV=1
RS20_PSEFS 58 10078 2 (1) 1 (1) 0.24
30S ribosomal protein S20 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsT PE=3 SV=1
RS20_PSEP1 58 10063 2 (1) 1 (1) 0.24
30S ribosomal protein S20 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsT PE=3 SV=1
RS20_PSEPF 58 9864 2 (1) 1 (1) 0.25
30S ribosomal protein S20 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsT PE=3 SV=1
RS20_PSEPG 58 10063 2 (1) 1 (1) 0.24
30S ribosomal protein S20 OS=Pseudomonas putida (strain GB-1) GN=rpsT PE=3 SV=1
RS20_PSEPK 58 10063 2 (1) 1 (1) 0.24
30S ribosomal protein S20 OS=Pseudomonas putida (strain KT2440) GN=rpsT PE=3 SV=1
RS20_PSEPW 58 10063 2 (1) 1 (1) 0.24
30S ribosomal protein S20 OS=Pseudomonas putida (strain W619) GN=rpsT PE=3 SV=1
RS20_PSESM 58 10006 2 (1) 1 (1) 0.24
30S ribosomal protein S20 OS=Pseudomonas syringae pv. tomato GN=rpsT PE=3 SV=1
RS20_PSEU2 58 10006 2 (1) 1 (1) 0.24
30S ribosomal protein S20 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsT PE=3 SV=1

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2247   743.4318 1484.8490 1484.8351 9.39 0 58 0.00013 +1Score > 36 indicates identity
Score > 32 indicates homology		 U K.AQAAYVLAVPVIDR.M
2249   743.9288 1485.8430 1485.8191 16.1 0 23 0.31 +1Score > 36 indicates identity
Score > 30 indicates homology		 U K.AQAAYVLAVPVIDR.M + Deamidated (NQ)

+85

Accession Score Description
1 RDRP_ACLSA 57 RNA-directed RNA polymerase OS=Apple chlorotic leaf spot virus (isolate apple) PE=4 SV=1

+86

Accession Score Description
1 TIG_PSEPK 55 Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2

+87

Accession Score Description
1 PUR5_PSEMY 55 Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas mendocina (strain ymp) GN=purM PE=3 SV=1

+88

Accession Score Description
1 MOAC_METB6 55 Probable molybdenum cofactor biosynthesis protein C OS=Methanoregula boonei (strain 6A8) GN=moaC PE=3 SV=1

+89

Accession Score Description
1 RS4_PSEPW 55 30S ribosomal protein S4 OS=Pseudomonas putida (strain W619) GN=rpsD PE=3 SV=1

+90

Accession Score Description
1 ISPD_ALKMQ 54 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase OS=Alkaliphilus metalliredigens (strain QYMF) GN=ispD PE=3 SV=1
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