MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 187)

Page: Previous 1 …4 5 6 7 8 9 10 11 12 13 14 … 19 Next 

+81

Accession Score Description
1 CLPX_CLOB8 59 ATP-dependent Clp protease ATP-binding subunit clpX OS=Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) GN=clpX PE=3 SV=1

+82

Accession Score Description
1 FLAE_VIBCH 59 Flagellin E OS=Vibrio cholerae GN=flaE PE=3 SV=1

-83

Accession Score Description
1 RS9_PSEE4 59 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1
Score Mass Matches Sequences emPAI
83.1 RS9_PSEE4 59 14597 6 (2) 3 (2) 0.35
30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1
10 samesets of RS9_PSEE4
RS9_PSEP1 59 14583 6 (2) 3 (2) 0.35
30S ribosomal protein S9 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsI PE=3 SV=1
RS9_PSEPG 59 14597 6 (2) 3 (2) 0.35
30S ribosomal protein S9 OS=Pseudomonas putida (strain GB-1) GN=rpsI PE=3 SV=1
RS9_PSEPK 59 14583 6 (2) 3 (2) 0.35
30S ribosomal protein S9 OS=Pseudomonas putida (strain KT2440) GN=rpsI PE=3 SV=1
RS9_PSE14 59 14593 3 (2) 2 (2) 0.35
30S ribosomal protein S9 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsI PE=3 SV=1
RS9_PSEF5 59 14610 3 (2) 2 (2) 0.35
30S ribosomal protein S9 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsI PE=3 SV=1
RS9_PSEFS 59 14607 3 (2) 2 (2) 0.35
30S ribosomal protein S9 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsI PE=3 SV=1
RS9_PSEMY 59 14637 3 (2) 2 (2) 0.35
30S ribosomal protein S9 OS=Pseudomonas mendocina (strain ymp) GN=rpsI PE=3 SV=1
RS9_PSEPF 59 14597 3 (2) 2 (2) 0.35
30S ribosomal protein S9 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsI PE=3 SV=1
RS9_PSESM 59 14593 3 (2) 2 (2) 0.35
30S ribosomal protein S9 OS=Pseudomonas syringae pv. tomato GN=rpsI PE=3 SV=1
RS9_PSEU2 59 14593 3 (2) 2 (2) 0.35
30S ribosomal protein S9 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsI PE=3 SV=1

-6 peptide matches (3 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
999 +2 470.7521 939.4896 939.4814 8.75 0 30 0.075 +1Score > 39 indicates identity
Score > 31 indicates homology		 U R.SLDVFFGR.E
1274   527.7513 1053.4880 1053.4839 3.91 0 42 0.0022 +1Score > 40 indicates identity
Score > 28 indicates homology		 U M.SATQNYGTGR.R
2640 +1 553.3102 1656.9088 1656.9060 1.69 0 43 0.0011 +1Score > 38 indicates identity
Score > 26 indicates homology		 U R.VFLRPGTGNISINNR.S

1 subset or intersection (7 subset proteins in total)

Score Mass Subset of
RS9_AZOVD 42 14723 83.1
30S ribosomal protein S9 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsI PE=3 SV=1
6 samesets of RS9_AZOVD
RS9_PSEA7 42 14616
30S ribosomal protein S9 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsI PE=3 SV=1
RS9_PSEA8 42 14606
30S ribosomal protein S9 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsI PE=3 SV=1
RS9_PSEAB 42 14606
30S ribosomal protein S9 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsI PE=3 SV=1
RS9_PSEAE 42 14606
30S ribosomal protein S9 OS=Pseudomonas aeruginosa GN=rpsI PE=3 SV=1
RS9_PSEPW 42 14581
30S ribosomal protein S9 OS=Pseudomonas putida (strain W619) GN=rpsI PE=3 SV=1
RS9_PSEU5 42 14604
30S ribosomal protein S9 OS=Pseudomonas stutzeri (strain A1501) GN=rpsI PE=3 SV=1

+84

Accession Score Description
1 RS20_PSE14 58 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1

+85

Accession Score Description
1 RDRP_ACLSA 57 RNA-directed RNA polymerase OS=Apple chlorotic leaf spot virus (isolate apple) PE=4 SV=1

+86

Accession Score Description
1 TIG_PSEPK 55 Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2

+87

Accession Score Description
1 PUR5_PSEMY 55 Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas mendocina (strain ymp) GN=purM PE=3 SV=1

+88

Accession Score Description
1 MOAC_METB6 55 Probable molybdenum cofactor biosynthesis protein C OS=Methanoregula boonei (strain 6A8) GN=moaC PE=3 SV=1

+89

Accession Score Description
1 RS4_PSEPW 55 30S ribosomal protein S4 OS=Pseudomonas putida (strain W619) GN=rpsD PE=3 SV=1

+90

Accession Score Description
1 ISPD_ALKMQ 54 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase OS=Alkaliphilus metalliredigens (strain QYMF) GN=ispD PE=3 SV=1
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