MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 187)


Page: 1 2 3 4 5 6  19 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1352 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
4 EFTU_CYAP7 34 Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1
3 EFTU_CARRP 180 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1236 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 1121 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSEFS 949 ATP synthase subunit beta OS=Pseudomonas fluorescens (strain SBW25) GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 954 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 173 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 163 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 667 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
4 CH602_SORC5 69 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
3 CH60_COLMA 82 60 kDa chaperonin OS=Colwellia maris GN=groL PE=3 SV=2
2 CH601_ECOK1 119 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1

+5

Accession Score Description
Family member distances as a dendrogram 1 RPOC_PSEPK 451 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1
4 RPOC_PELUB 108 DNA-directed RNA polymerase subunit beta' OS=Pelagibacter ubique GN=rpoC PE=3 SV=1
3 RPOC_SYNAS 149 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
2 RPOB_PSEP1 316 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoB PE=3 SV=1

+6

Accession Score Description
1 RL1_PSEE4 451 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+7

Accession Score Description
1 OTCC_PSEPK 445 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

-8

Accession Score Description
1 ARCA_PSEPK 405 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1
Score Mass Matches Sequences emPAI
8.1 ARCA_PSEPK 405 46775 28 (13) 13 (6) 0.40
Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

-28 peptide matches (17 non-duplicate, 11 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
491 +1 351.7018 701.3890 701.3860 4.28 0 30 0.12 +1Score > 42 indicates identity
Score > 33 indicates homology
K.WILDR.K
969   925.5739 924.5666 924.5644 2.40 1 30 0.14 +1Score > 34 indicates identity U K.EVIVAGLPK.S
970 +1 463.2914 924.5682 924.5644 4.15 1 23 0.23 +1Score > 34 indicates identity
Score > 29 indicates homology
U K.EVIVAGLPK.S
1020   316.4915 946.4527 946.4508 1.94 1 12 3 +6Score > 42 indicates identity
Score > 29 indicates homology
U K.YGVHSEAGK.L
1022   474.2356 946.4566 946.4508 6.14 1 43 0.011 +1Score > 42 indicates identity
Score > 35 indicates homology
U K.YGVHSEAGK.L
1171   504.7436 1007.4726 1007.4713 1.37 0 22 0.85 +1Score > 42 indicates identity
Score > 33 indicates homology
U R.DHFDFVTK.M
1341 +1 543.7883 1085.5620 1085.5505 10.6 1 37 0.071 +1Score > 41 indicates identity
Score > 38 indicates homology
U R.SWLEGLEPR.H
1354 +2 548.2964 1094.5782 1094.5720 5.70 0 57 0.00099 +1Score > 39 indicates identity U R.NTYTNTLLR.K
1738 +1 623.3646 1244.7146 1244.7017 10.4 1 12 0.15 +1Score > 37 indicates identity
Score > 16 indicates homology
U R.DLVTVFPEVVK.E
1816   640.8595 1279.7044 1279.7023 1.64 1 15 0.73 +1Score > 38 indicates identity
Score > 26 indicates homology
U R.QETLLTTAIYK.F
1824   641.3600 1280.7054 1280.6864 14.9 1 10 1.2 +2Score > 38 indicates identity
Score > 23 indicates homology
U R.QETLLTTAIYK.F + Deamidated (NQ)
2080 +2 701.4053 1400.7960 1400.7776 13.2 0 91 1.5e-007 +1Score > 37 indicates identity
Score > 35 indicates homology
U R.QAIGQLAQNLFAK.G
2436   524.2897 1569.8473 1569.8363 7.02 1 57 5.8e-005 +1Score > 39 indicates identity
Score > 27 indicates homology
U K.ITPDTVGVGLTNEVR.S
2437 +2 785.9312 1569.8478 1569.8363 7.38 1 77 6e-006 +1Score > 39 indicates identity
Score > 37 indicates homology
U K.ITPDTVGVGLTNEVR.S
3257   1019.5556 2037.0966 2037.0565 19.7 1 88 3.5e-008 +1Score > 36 indicates identity
Score > 26 indicates homology
U R.GVDVLEMHNLLTDIVQNK.E
3883 +1 816.0710 2445.1912 2445.1448 19.0 1 48 0.0066 +1Score > 39 indicates identity U R.EQWDDGNNVVAIEPGVVIGYDR.N + Deamidated (NQ)
4108   904.7930 2711.3572 2711.3054 19.1 0 19 0.06 +1Score > 37 indicates identity
Score > 19 indicates homology
U K.MYNDYLGHSSFILPPLPNTQFTR.D + Deamidated (NQ)

2 subsets and intersections (12 subset proteins in total)

Score Mass Subset of
ARCA_PSEPU 99 46933 8.1
Arginine deiminase OS=Pseudomonas putida GN=arcA PE=1 SV=1
ARCA1_RHIME 76 46140 8.1
Arginine deiminase 1 OS=Rhizobium meliloti GN=arcA1 PE=3 SV=1
10 samesets of ARCA1_RHIME
ARCA_PSEAE 76 46806
Arginine deiminase OS=Pseudomonas aeruginosa GN=arcA PE=1 SV=2
ARCA_RHIET 76 46140
Arginine deiminase OS=Rhizobium etli GN=arcA PE=3 SV=1
ARCA_RHIE6 76 46196
Arginine deiminase OS=Rhizobium etli (strain CIAT 652) GN=arcA PE=3 SV=1
ARCA_BURMA 76 46422
Arginine deiminase OS=Burkholderia mallei GN=arcA PE=3 SV=1
ARCA_BURPS 76 46422
Arginine deiminase OS=Burkholderia pseudomallei GN=arcA PE=3 SV=1
ARCA_PSEMY 76 46334
Arginine deiminase OS=Pseudomonas mendocina (strain ymp) GN=arcA PE=3 SV=1
ARCA_BRAJA 76 46780
Arginine deiminase OS=Bradyrhizobium japonicum GN=arcA PE=3 SV=1
ARCA_MARMS 76 46613
Arginine deiminase OS=Marinomonas sp. (strain MWYL1) GN=arcA PE=3 SV=1
ARCA_RHILO 76 46031
Arginine deiminase OS=Rhizobium loti GN=arcA PE=3 SV=1
ARCA2_RHIME 76 46880
Arginine deiminase 2 OS=Rhizobium meliloti GN=arcA2 PE=3 SV=1

+9

Accession Score Description
1 DLDH2_PSEPU 387 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+10

Accession Score Description
1 DBHB_PSEAE 318 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3
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