MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 71–80 (out of 187)


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+71

Accession Score Description
1 CYOA_PSEPU 71 Ubiquinol oxidase subunit 2 OS=Pseudomonas putida GN=cyoA PE=3 SV=1

+72

Accession Score Description
1 ODP1_PSEAE 69 Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2

+73

Accession Score Description
1 ASSY_PSEPG 68 Argininosuccinate synthase OS=Pseudomonas putida (strain GB-1) GN=argG PE=3 SV=1

+74

Accession Score Description
1 CH10_PSE14 68 10 kDa chaperonin OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=groS PE=3 SV=1

+75

Accession Score Description
Family member distances as a dendrogram 1 RL20_PSEE4 67 50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1
2 RL20_POLNS 49 50S ribosomal protein L20 OS=Polynucleobacter necessarius (strain STIR1) GN=rplT PE=3 SV=1

+76

Accession Score Description
1 CLPP_PSEPG 66 ATP-dependent Clp protease proteolytic subunit OS=Pseudomonas putida (strain GB-1) GN=clpP PE=3 SV=1

-77

Accession Score Description
1 ATPE_PSEPG 64 ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1
Score Mass Matches Sequences emPAI
77.1 ATPE_PSEPG 64 14606 5 (3) 4 (2) 0.35
ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1
2 samesets of ATPE_PSEPG
ATPE_PSEPK 64 14585 5 (3) 4 (2) 0.35
ATP synthase epsilon chain OS=Pseudomonas putida (strain KT2440) GN=atpC PE=3 SV=1
ATPE_PSEPW 64 14595 4 (3) 3 (2) 0.35
ATP synthase epsilon chain OS=Pseudomonas putida (strain W619) GN=atpC PE=3 SV=1

-5 peptide matches (4 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
920   451.2603 900.5060 900.5029 3.51 0 23 1.6 +8Score > 42 indicates identity
Score > 38 indicates homology
U K.VLADTVQR.A
1009 +1 471.7739 941.5332 941.5294 4.10 1 45 0.016 +1Score > 39 indicates identity U R.LAEAAAQLR.T
1013   472.2662 942.5178 942.5134 4.72 1 25 0.73 +1Score > 41 indicates identity
Score > 36 indicates homology
U K.AAENALNLK.G
1566   592.7720 1183.5294 1183.5258 3.09 0 52 0.0009 +1Score > 39 indicates identity
Score > 34 indicates homology
U K.GADFDYGAAAAR.L

1 subset or intersection (20 subset proteins in total)

Score Mass Subset of
ATPE_COXB1 47 15277 77.1
ATP synthase epsilon chain OS=Coxiella burnetii (strain CbuK_Q154) GN=atpC PE=3 SV=1
19 samesets of ATPE_COXB1
ATPE_PSEP1 47 14636
ATP synthase epsilon chain OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpC PE=3 SV=1
ATPE_PSEFS 47 14615
ATP synthase epsilon chain OS=Pseudomonas fluorescens (strain SBW25) GN=atpC PE=3 SV=1
ATPE_PSEPF 47 14802
ATP synthase epsilon chain OS=Pseudomonas fluorescens (strain Pf0-1) GN=atpC PE=3 SV=1
ATPE_PSESM 47 14607
ATP synthase epsilon chain OS=Pseudomonas syringae pv. tomato GN=atpC PE=3 SV=1
ATPE_PSEU2 47 14794
ATP synthase epsilon chain OS=Pseudomonas syringae pv. syringae (strain B728a) GN=atpC PE=3 SV=1
ATPE_PSEE4 47 14635
ATP synthase epsilon chain OS=Pseudomonas entomophila (strain L48) GN=atpC PE=3 SV=1
ATPE_PSEF5 47 14853
ATP synthase epsilon chain OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=atpC PE=3 SV=1
ATPE_PSE14 47 14794
ATP synthase epsilon chain OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpC PE=3 SV=1
ATPE_MARAV 47 15117
ATP synthase epsilon chain OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=atpC PE=3 SV=1
ATPE_COXBN 47 15277
ATP synthase epsilon chain OS=Coxiella burnetii (strain Dugway 5J108-111) GN=atpC PE=3 SV=1
ATPE_COXBR 47 15277
ATP synthase epsilon chain OS=Coxiella burnetii (strain RSA 331 / Henzerling II) GN=atpC PE=3 SV=1
ATPE_COXBU 47 15277
ATP synthase epsilon chain OS=Coxiella burnetii GN=atpC PE=3 SV=1
ATPE_PSEAB 47 14774
ATP synthase epsilon chain OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=atpC PE=3 SV=1
ATPE_PSEAE 47 14774
ATP synthase epsilon chain OS=Pseudomonas aeruginosa GN=atpC PE=3 SV=1
ATPE_PSEMY 47 15370
ATP synthase epsilon chain OS=Pseudomonas mendocina (strain ymp) GN=atpC PE=3 SV=1
ATPE_TERTT 47 14938
ATP synthase epsilon chain OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=atpC PE=3 SV=1
ATPE_COXB2 47 15277
ATP synthase epsilon chain OS=Coxiella burnetii (strain CbuG_Q212) GN=atpC PE=3 SV=1
ATPE_PSEA7 47 14774
ATP synthase epsilon chain OS=Pseudomonas aeruginosa (strain PA7) GN=atpC PE=3 SV=1
ATPE_PSEA8 47 14774
ATP synthase epsilon chain OS=Pseudomonas aeruginosa (strain LESB58) GN=atpC PE=3 SV=1

+78

Accession Score Description
1 RPOZ_ALCBS 63 DNA-directed RNA polymerase subunit omega OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=rpoZ PE=3 SV=1

+79

Accession Score Description
1 RL15_PSEP1 61 50S ribosomal protein L15 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplO PE=3 SV=1

+80

Accession Score Description
1 CEP1_CAEEL 59 Transcription factor cep-1 OS=Caenorhabditis elegans GN=cep-1 PE=1 SV=2
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