MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 71–80 (out of 187)

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+71

Accession Score Description
1 CYOA_PSEPU 71 Ubiquinol oxidase subunit 2 OS=Pseudomonas putida GN=cyoA PE=3 SV=1

+72

Accession Score Description
1 ODP1_PSEAE 69 Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2

+73

Accession Score Description
1 ASSY_PSEPG 68 Argininosuccinate synthase OS=Pseudomonas putida (strain GB-1) GN=argG PE=3 SV=1

+74

Accession Score Description
1 CH10_PSE14 68 10 kDa chaperonin OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=groS PE=3 SV=1

-75

Accession Score Description
Family member distances as a dendrogram 1 RL20_PSEE4 67 50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1
2 RL20_POLNS 49 50S ribosomal protein L20 OS=Polynucleobacter necessarius (strain STIR1) GN=rplT PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
75.1 RL20_PSEE4 67 13272 7 (2) 5 (2) 0.39
50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1
13 samesets of RL20_PSEE4
RL20_PSEF5 67 13287 7 (2) 5 (2) 0.39
50S ribosomal protein L20 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplT PE=3 SV=1
RL20_PSEFL 67 13287 7 (2) 5 (2) 0.39
50S ribosomal protein L20 OS=Pseudomonas fluorescens GN=rplT PE=3 SV=1
RL20_PSEMY 67 13241 7 (2) 5 (2) 0.39
50S ribosomal protein L20 OS=Pseudomonas mendocina (strain ymp) GN=rplT PE=3 SV=1
RL20_PSEP1 67 13272 7 (2) 5 (2) 0.39
50S ribosomal protein L20 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplT PE=3 SV=1
RL20_PSEPG 67 13272 7 (2) 5 (2) 0.39
50S ribosomal protein L20 OS=Pseudomonas putida (strain GB-1) GN=rplT PE=3 SV=1
RL20_PSEPK 67 13272 7 (2) 5 (2) 0.39
50S ribosomal protein L20 OS=Pseudomonas putida (strain KT2440) GN=rplT PE=3 SV=1
RL20_PSEPW 67 13272 7 (2) 5 (2) 0.39
50S ribosomal protein L20 OS=Pseudomonas putida (strain W619) GN=rplT PE=3 SV=1
RL20_PSEU5 67 13284 7 (2) 5 (2) 0.39
50S ribosomal protein L20 OS=Pseudomonas stutzeri (strain A1501) GN=rplT PE=3 SV=1
RL20_PSE14 67 13306 6 (2) 4 (2) 0.39
50S ribosomal protein L20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplT PE=3 SV=1
RL20_PSEPF 67 13306 6 (2) 4 (2) 0.39
50S ribosomal protein L20 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplT PE=3 SV=1
RL20_PSESM 67 13306 6 (2) 4 (2) 0.39
50S ribosomal protein L20 OS=Pseudomonas syringae pv. tomato GN=rplT PE=3 SV=1
RL20_PSESY 67 13306 6 (2) 4 (2) 0.39
50S ribosomal protein L20 OS=Pseudomonas syringae pv. syringae GN=rplT PE=3 SV=1
RL20_PSEU2 67 13306 6 (2) 4 (2) 0.39
50S ribosomal protein L20 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplT PE=3 SV=1
75.2 RL20_POLNS 49 13738 3 (2) 2 (1) 0.17
50S ribosomal protein L20 OS=Polynucleobacter necessarius (strain STIR1) GN=rplT PE=3 SV=1
1 sameset of RL20_POLNS
RL20_POLSQ 49 13742 3 (2) 2 (1) 0.17
50S ribosomal protein L20 OS=Polynucleobacter sp. (strain QLW-P1DMWA-1) GN=rplT PE=3 SV=1

+8 peptide matches (6 non-duplicate, 2 duplicate)

2 subsets and intersections (7 subset proteins in total)

Score Mass Subset of
RL20_PSEA7 64 13357 75.1
50S ribosomal protein L20 OS=Pseudomonas aeruginosa (strain PA7) GN=rplT PE=3 SV=1
3 samesets of RL20_PSEA7
RL20_PSEA8 64 13357
50S ribosomal protein L20 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplT PE=3 SV=1
RL20_PSEAB 64 13357
50S ribosomal protein L20 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplT PE=3 SV=1
RL20_PSEAE 64 13357
50S ribosomal protein L20 OS=Pseudomonas aeruginosa GN=rplT PE=3 SV=1
RL20_AZOVD 35 13385 75.1
50S ribosomal protein L20 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplT PE=3 SV=1
2 samesets of RL20_AZOVD
RL20_AZOVI 35 13429
50S ribosomal protein L20 OS=Azotobacter vinelandii GN=rplT PE=3 SV=1
RL20_PROMH 35 13449
50S ribosomal protein L20 OS=Proteus mirabilis (strain HI4320) GN=rplT PE=3 SV=1

+76

Accession Score Description
1 CLPP_PSEPG 66 ATP-dependent Clp protease proteolytic subunit OS=Pseudomonas putida (strain GB-1) GN=clpP PE=3 SV=1

+77

Accession Score Description
1 ATPE_PSEPG 64 ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1

+78

Accession Score Description
1 RPOZ_ALCBS 63 DNA-directed RNA polymerase subunit omega OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=rpoZ PE=3 SV=1

+79

Accession Score Description
1 RL15_PSEP1 61 50S ribosomal protein L15 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplO PE=3 SV=1

+80

Accession Score Description
1 CEP1_CAEEL 59 Transcription factor cep-1 OS=Caenorhabditis elegans GN=cep-1 PE=1 SV=2
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