MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 187)

Page: 1 2 3 4 5 6 … 19 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1352 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
4 EFTU_CYAP7 34 Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1
3 EFTU_CARRP 180 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1236 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 1121 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSEFS 949 ATP synthase subunit beta OS=Pseudomonas fluorescens (strain SBW25) GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 954 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 173 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 163 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 667 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
4 CH602_SORC5 69 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
3 CH60_COLMA 82 60 kDa chaperonin OS=Colwellia maris GN=groL PE=3 SV=2
2 CH601_ECOK1 119 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1

+5

Accession Score Description
Family member distances as a dendrogram 1 RPOC_PSEPK 451 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1
4 RPOC_PELUB 108 DNA-directed RNA polymerase subunit beta' OS=Pelagibacter ubique GN=rpoC PE=3 SV=1
3 RPOC_SYNAS 149 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
2 RPOB_PSEP1 316 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoB PE=3 SV=1

+6

Accession Score Description
1 RL1_PSEE4 451 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

-7

Accession Score Description
1 OTCC_PSEPK 445 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3
Score Mass Matches Sequences emPAI
7.1 OTCC_PSEPK 445 38115 46 (29) 15 (10) 1.56
Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

-46 peptide matches (24 non-duplicate, 22 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
205 +1 499.3254 498.3181 498.3166 3.14 0 31 0.079 +1Score > 34 indicates identity
Score > 32 indicates homology		 R.IAAPK.A
464 +1 345.6849 689.3552 689.3530 3.20 0 36 0.25 +1Score > 43 indicates identity K.LGMDVR.I
847 +1 436.2316 870.4486 870.4460 3.03 0 38 0.06 +1Score > 39 indicates identity
Score > 38 indicates homology		 U M.AFNIHNR.N
868 +2 440.2532 878.4918 878.4861 6.49 0 33 0.21 +5Score > 38 indicates identity U R.YLLDLSR.D
869   879.5000 878.4927 878.4861 7.49 0 45 0.0087 +1Score > 37 indicates identity U R.YLLDLSR.D
942 +2 458.7528 915.4910 915.4913 -0.28 1 45 0.0081 +1Score > 43 indicates identity
Score > 37 indicates homology		 U R.ITLTEDPK.A
943   916.5012 915.4939 915.4913 2.86 1 50 0.004 +1Score > 43 indicates identity
Score > 39 indicates homology		 U R.ITLTEDPK.A
1193 +3 1015.6124 1014.6051 1014.5961 8.89 0 15 1.3 +2Score > 37 indicates identity
Score > 29 indicates homology		 U K.AILVSTLADL.-
1198   508.3116 1014.6086 1014.5961 12.4 0 9 0.32 +1Score > 37 indicates identity
Score > 17 indicates homology		 U K.AILVSTLADL.-
1290   530.7441 1059.4736 1059.4695 3.92 1 39 0.052 +1Score > 39 indicates identity U R.MYDAIEYR.G
1395 +1 1118.6289 1117.6216 1117.6131 7.60 1 68 8e-005 +1Score > 39 indicates identity U K.GNNIALIFEK.T
1399 +2 559.8201 1117.6256 1117.6131 11.2 1 56 0.00076 +1Score > 39 indicates identity
Score > 37 indicates homology		 U K.GNNIALIFEK.T
1615   602.8032 1203.5918 1203.5884 2.87 1 45 0.0042 +1Score > 42 indicates identity
Score > 34 indicates homology		 U K.YTGTEQQHLK.G
1616 +1 402.2048 1203.5926 1203.5884 3.47 1 38 0.0014 +1Score > 42 indicates identity
Score > 22 indicates homology		 U K.YTGTEQQHLK.G
1901 +1 330.9318 1319.6981 1319.6946 2.66 1 47 0.001 +1Score > 41 indicates identity
Score > 30 indicates homology		 U R.NLLSLEHHTTR.E
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_6_20250714122635.raw

Score > 41 indicates identity

Score > 32 indicates homology
1902   660.8564 1319.6982 1319.6946 2.78 1 42 0.0049 -1Score > 41 indicates identity
Score > 32 indicates homology		 U R.NLLSLEHHTTR.E
2.78 0 17 1.8 2 VQQSPHQNILR   + Deamidated (NQ)
16.2 0 16 2 3 NLLSLMHHSPR   + Oxidation (M)
-7.75 1 9 10 4 LNIFNQQSVKK   + 2 Deamidated (NQ)
-7.75 1 9 10 4 LNIFNQQSVKK   + 2 Deamidated (NQ)
-18.8 1 9 11 6 VAGLKMSVSATTR  
19.8 0 9 11 7 LNISYPVNGSQK   + Deamidated (NQ)
11.3 1 8 12 8 VAGLYGVAAESGAR  
19.8 0 8 12 9 LNISYPVNGSQK   + Deamidated (NQ)
-16.3 1 8 14 10 ARLATFQTAVNK   + Deamidated (NQ)
1903 +3 440.9068 1319.6986 1319.6946 3.02 1 39 0.0018 +1Score > 40 indicates identity
Score > 24 indicates homology		 U R.NLLSLEHHTTR.E
1908   441.2403 1320.6991 1320.6786 15.5 1 20 0.25 +1Score > 40 indicates identity
Score > 27 indicates homology		 U R.NLLSLEHHTTR.E + Deamidated (NQ)
1953 +1 672.8757 1343.7368 1343.7231 10.2 0 67 9.3e-005 +1Score > 39 indicates identity U R.NNMGNSLLLIGAK.L
1965 +1 675.8463 1349.6780 1349.6728 3.90 1 38 0.0032 +1Score > 41 indicates identity
Score > 25 indicates homology		 U K.ALWPHDDLVER.C
1968 +2 450.9010 1349.6812 1349.6728 6.21 1 42 0.0012 +1Score > 41 indicates identity
Score > 25 indicates homology		 U K.ALWPHDDLVER.C
2414   521.2874 1560.8404 1560.8334 4.49 1 19 0.45 +1Score > 39 indicates identity
Score > 28 indicates homology		 U K.QLKPYQVNAELMK.S
3517   729.6898 2186.0476 2186.0392 3.82 1 84 4.7e-008 +1Score > 39 indicates identity
Score > 24 indicates homology		 U R.EHSDKPLHDISYAYLGDAR.N
4351   1008.5127 3022.5163 3022.4569 19.7 1 14 0.067 +1Score > 36 indicates identity
Score > 15 indicates homology		 U K.FAGVPVFNGLTDEYHPTQMIADVLTMR.E + Deamidated (NQ)

9 subsets and intersections (26 subset proteins in total)

Score Mass Subset of
OTCC_PSEAE 217 38255 7.1
Ornithine carbamoyltransferase, catabolic OS=Pseudomonas aeruginosa GN=arcB PE=1 SV=3
OTCC_PSEME 157 38189 7.1
Ornithine carbamoyltransferase, catabolic OS=Pseudomonas mendocina GN=arcB PE=3 SV=3
OTCC_HAEIN 99 37908 7.1
Ornithine carbamoyltransferase, catabolic OS=Haemophilus influenzae GN=arcB PE=3 SV=1
1 sameset of OTCC_HAEIN
OTC_HAEIG 99 37881
Ornithine carbamoyltransferase OS=Haemophilus influenzae (strain PittGG) GN=arcB PE=3 SV=1
OTCC_BURMA 92 38278 7.1
Ornithine carbamoyltransferase, catabolic OS=Burkholderia mallei GN=arcB PE=3 SV=1
3 samesets of OTCC_BURMA
OTCC_BURPS 92 38278
Ornithine carbamoyltransferase, catabolic OS=Burkholderia pseudomallei GN=arcB PE=3 SV=1
OTCC_HAEGA 92 37705
Ornithine carbamoyltransferase, catabolic OS=Haemophilus gallinarum GN=arcB PE=3 SV=1
OTC_HAEDU 92 37722
Ornithine carbamoyltransferase OS=Haemophilus ducreyi GN=argF PE=3 SV=1
OTC_MANSM 90 37793 7.1
Ornithine carbamoyltransferase OS=Mannheimia succiniciproducens (strain MBEL55E) GN=argF PE=3 SV=1
OTCC_CHRVO 68 38025 7.1
Ornithine carbamoyltransferase, catabolic OS=Chromobacterium violaceum GN=arcB PE=3 SV=1
OTCC_MYCCC 45 35232 7.1
Ornithine carbamoyltransferase, catabolic OS=Mycoplasma capricolum subsp. capripneumoniae GN=arcB PE=3 SV=1
1 sameset of OTCC_MYCCC
THI22_YEAST 45 64521
Thiamine biosynthesis protein THI22 OS=Saccharomyces cerevisiae GN=THI22 PE=2 SV=1
OTCC_RHIET 39 38135 7.1
Ornithine carbamoyltransferase, catabolic OS=Rhizobium etli GN=arcB PE=3 SV=1
12 samesets of OTCC_RHIET
OTC_ACTPJ 39 37600
Ornithine carbamoyltransferase OS=Actinobacillus pleuropneumoniae serotype 3 (strain JL03) GN=arcB PE=3 SV=1
OTC_NEIGO 39 36823
Ornithine carbamoyltransferase OS=Neisseria gonorrhoeae GN=argF PE=3 SV=1
OTC_NEILA 39 28761
Ornithine carbamoyltransferase (Fragment) OS=Neisseria lactamica GN=argF PE=3 SV=1
OTC_NEIMA 39 36795
Ornithine carbamoyltransferase OS=Neisseria meningitidis serogroup A GN=argF PE=3 SV=1
OTC_NEIMB 39 36807
Ornithine carbamoyltransferase OS=Neisseria meningitidis serogroup B GN=argF PE=3 SV=1
OTC_NEIME 39 28974
Ornithine carbamoyltransferase (Fragment) OS=Neisseria meningitidis GN=argF PE=3 SV=2
OTC_NEIPE 39 25521
Ornithine carbamoyltransferase (Fragment) OS=Neisseria perflava GN=argF PE=3 SV=1
OTC_NEIPO 39 28876
Ornithine carbamoyltransferase (Fragment) OS=Neisseria polysaccharea GN=argF PE=3 SV=1
OTC_NEIG1 39 36768
Ornithine carbamoyltransferase OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=argF PE=3 SV=1
OTC_ACTP2 39 37600
Ornithine carbamoyltransferase OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=argF PE=3 SV=1
OTC_ACTP7 39 37600
Ornithine carbamoyltransferase OS=Actinobacillus pleuropneumoniae serotype 7 (strain AP76) GN=arcB PE=3 SV=1
OTCC_RHIME 39 37978
Ornithine carbamoyltransferase, catabolic OS=Rhizobium meliloti GN=arcB PE=3 SV=1
OTCC_PSEPU 38 4144 7.1
Ornithine carbamoyltransferase, catabolic (Fragment) OS=Pseudomonas putida GN=arcB PE=1 SV=2

+8

Accession Score Description
1 ARCA_PSEPK 405 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+9

Accession Score Description
1 DLDH2_PSEPU 387 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+10

Accession Score Description
1 DBHB_PSEAE 318 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3
Page: 1 2 3 4 5 6 … 19 Next 

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