MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 187)


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+61

Accession Score Description
1 RL7_PSEP1 87 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplL PE=3 SV=1

+62

Accession Score Description
1 SUCD_PSEAE 87 Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2

+63

Accession Score Description
1 ACCA_PSEPK 80 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=accA PE=3 SV=1

+64

Accession Score Description
1 CISY_PSEAE 79 Citrate synthase OS=Pseudomonas aeruginosa GN=gltA PE=3 SV=2

-65

Accession Score Description
1 RL14_PSE14 77 50S ribosomal protein L14 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplN PE=3 SV=1
Score Mass Matches Sequences emPAI
65.1 RL14_PSE14 77 13458 9 (3) 5 (2) 0.39
50S ribosomal protein L14 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplN PE=3 SV=1
+51 samesets of RL14_PSE14

-9 peptide matches (5 non-duplicate, 4 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
674 +1 395.2362 788.4578 788.4545 4.27 0 26 0.86 +1Score > 39 indicates identity
Score > 38 indicates homology
R.IFGPVTR.E
699   400.7182 799.4218 799.4188 3.80 0 17 1.2 +2Score > 39 indicates identity
Score > 31 indicates homology
U K.QEPIGTR.I
1004 +1 940.5813 939.5740 939.5641 10.6 1 25 0.034 +1Score > 35 indicates identity
Score > 23 indicates homology
U K.IVSLAPEVL.-
1038 +1 475.2755 948.5364 948.5280 8.89 0 34 0.13 +1Score > 39 indicates identity
Score > 38 indicates homology
R.YAGIGDIIK.V
1798 +1 638.3419 1274.6692 1274.6619 5.78 0 63 0.00035 +1Score > 41 indicates identity U R.FDGNAAVLLNNK.Q

1 subset or intersection (460 subset proteins in total)

Score Mass Subset of
RK14_ACOCL 25 13643 65.1
50S ribosomal protein L14, chloroplastic OS=Acorus calamus GN=rpl14 PE=3 SV=1
+459 samesets of RK14_ACOCL

+66

Accession Score Description
1 ASPQ_PSEPK 77 Glutaminase-asparaginase OS=Pseudomonas putida (strain KT2440) GN=ansB PE=3 SV=1

+67

Accession Score Description
1 ETFB_PSEAE 77 Electron transfer flavoprotein subunit beta OS=Pseudomonas aeruginosa GN=etfB PE=3 SV=1

+68

Accession Score Description
1 PURA_PSEPG 76 Adenylosuccinate synthetase OS=Pseudomonas putida (strain GB-1) GN=purA PE=3 SV=1

+69

Accession Score Description
1 PHAA_PSEOL 74 Poly(3-hydroxyalkanoate) polymerase 1 OS=Pseudomonas oleovorans GN=phaA PE=3 SV=1

+70

Accession Score Description
1 KPRS_PSEPK 73 Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1
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