MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita6 sp |
| MS data file | : | PRT1270_T-BRSC_6_20250714122635.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:38:56 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,869 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU2_PSEPK | 1352 | Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1 | |
| 4 | EFTU_CYAP7 | 34 | Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1 | |
| 3 | EFTU_CARRP | 180 | Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1 | |
| 2 | EFTU1_PSEPK | 1236 | Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_PSEPG | 1121 | ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1 | |
| 2 | ATPB_PSEFS | 949 | ATP synthase subunit beta OS=Pseudomonas fluorescens (strain SBW25) GN=atpD PE=3 SV=1 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPA_PSEPK | 954 | ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1 | |
| 2 | ATPA_RICAH | 173 | ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1 | |
| 3 | ATPA_VEREI | 163 | ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1 | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_PSEPK | 667 | 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1 | |
| 4 | CH602_SORC5 | 69 | 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1 | |
| 3 | CH60_COLMA | 82 | 60 kDa chaperonin OS=Colwellia maris GN=groL PE=3 SV=2 | |
| 2 | CH601_ECOK1 | 119 | 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1 | |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOC_PSEPK | 451 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1 | |
| 4 | RPOC_PELUB | 108 | DNA-directed RNA polymerase subunit beta' OS=Pelagibacter ubique GN=rpoC PE=3 SV=1 | |
| 3 | RPOC_SYNAS | 149 | DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1 | |
| 2 | RPOB_PSEP1 | 316 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoB PE=3 SV=1 | |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL1_PSEE4 | 451 | 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 6.1 | RL1_PSEE4 |
451 | 24208 | 15 (11) | 6 (5) | 0.90 | |
| 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1 | |||||||
| 2 samesets of RL1_PSEE4 | |||||||
| RL1_PSEP1 |
451 | 24236 | 15 (11) | 6 (5) | 0.90 | ||
| 50S ribosomal protein L1 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplA PE=3 SV=1 | |||||||
| RL1_PSEPK |
451 | 24236 | 15 (11) | 6 (5) | 0.90 | ||
| 50S ribosomal protein L1 OS=Pseudomonas putida (strain KT2440) GN=rplA PE=3 SV=1 | |||||||
15 peptide matches (11 non-duplicate, 4 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 1218 |
342.5306 | 1024.5700 | 1024.5665 | 3.35 | 0 | 34 | 0.11 | 1Score > 37 indicates identity Score > 37 indicates homology |
U | K.NGIIHTSVGK.V | |
| 1299 |
534.2978 | 1066.5810 | 1066.5771 | 3.68 | 0 | 30 | 0.055 | 1Score > 38 indicates identity Score > 30 indicates homology |
U | R.SATVLPHGTGK.T | |
| 1300 |
356.5344 | 1066.5814 | 1066.5771 | 3.99 | 0 | 26 | 0.13 | 1Score > 38 indicates identity Score > 30 indicates homology |
U | R.SATVLPHGTGK.T | |
| 1668 |
2 |
611.8710 | 1221.7274 | 1221.7194 | 6.61 | 0 | 65 | 4.4e-006 | 1Score > 32 indicates identity Score > 24 indicates homology |
U | R.VVGQLGQVLGPR.G |
| 1762 |
629.3589 | 1256.7032 | 1256.6976 | 4.46 | 0 | 61 | 4.5e-005 | 1Score > 39 indicates identity Score > 30 indicates homology |
U | K.VGTVTPDVATAVK.N | |
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_6_20250714122635.rawScore > 40 indicates identity Score > 23 indicates homology |
|||||||||||
| 2888 |
897.4644 | 1792.9142 | 1792.8996 | 8.19 | 1 | 45 | 0.00036 | 1Score > 40 indicates identity Score > 23 indicates homology |
U | K.FVESYDIAVNLGVDPR.K | |
| 10.5 | 0 | 8 | 1.9 | 2 | RPALQDSNLAPSLGPGAE + Deamidated (NQ) | ||||||
| 10.5 | 0 | 8 | 1.9 | 2 | RPALQDSNLAPSLGPGAE + Deamidated (NQ) | ||||||
| -10.3 | 1 | 3 | 5.5 | 4 | MNTFLIIDGMNLVRR + Deamidated (NQ) | ||||||
| -6.22 | 1 | 3 | 5.9 | 5 | VFSNAATMQGGRVVALR + Deamidated (NQ); Oxidation (M) | ||||||
| -3.59 | 1 | 3 | 6.1 | 6 | DKPADVTPGQIELAPDK | ||||||
| 6.31 | 0 | 3 | 6.3 | 7 | ADMGTVDLVVYSLASPR | ||||||
| 6.32 | 1 | 3 | 6.3 | 7 | EMGQVDLVIYSLASPR + Oxidation (M) | ||||||
| -18.4 | 1 | 3 | 6.4 | 9 | DFLSHRIVGEPPANIK + Deamidated (NQ) | ||||||
| -7.69 | 1 | 2 | 6.8 | 10 | SSPKQTPMIAQYLSIK + 2 Deamidated (NQ) | ||||||
| 2889 |
598.6479 | 1792.9219 | 1792.8996 | 12.4 | 1 | 9 | 1.2 | 2Score > 40 indicates identity Score > 22 indicates homology |
U | K.FVESYDIAVNLGVDPR.K | |
| 2890 |
897.9669 | 1793.9192 | 1793.8836 | 19.9 | 1 | 26 | 0.075 | 1Score > 40 indicates identity Score > 27 indicates homology |
U | K.FVESYDIAVNLGVDPR.K + Deamidated (NQ) | |
| 3047 |
943.5010 | 1884.9874 | 1884.9694 | 9.60 | 1 | 108 | 1.5e-009 | 1Score > 39 indicates identity Score > 33 indicates homology |
U | R.VAVFTQGPAAEAALAAGADR.V | |
| 3048 |
1 |
629.3381 | 1884.9925 | 1884.9694 | 12.3 | 1 | 67 | 9.6e-006 | 1Score > 38 indicates identity Score > 29 indicates homology |
U | R.VAVFTQGPAAEAALAAGADR.V |
| 3050 |
1 |
944.0011 | 1885.9876 | 1885.9534 | 18.2 | 1 | 118 | 8.3e-011 | 1Score > 39 indicates identity Score > 30 indicates homology |
U | R.VAVFTQGPAAEAALAAGADR.V + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| RL1_PSEPW |
429 | 24112 | 6.1 | ||
| 50S ribosomal protein L1 OS=Pseudomonas putida (strain W619) GN=rplA PE=3 SV=1 | |||||
| 1 sameset of RL1_PSEPW | |||||
| RL1_PSESM |
429 | 24163 | |||
| 50S ribosomal protein L1 OS=Pseudomonas syringae pv. tomato GN=rplA PE=3 SV=1 | |||||
| RL1_PSEPG |
419 | 24180 | 6.1 | ||
| 50S ribosomal protein L1 OS=Pseudomonas putida (strain GB-1) GN=rplA PE=3 SV=1 | |||||
| RL1_PSE14 |
398 | 24120 | 6.1 | ||
| 50S ribosomal protein L1 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplA PE=3 SV=1 | |||||
| 2 samesets of RL1_PSE14 | |||||
| RL1_PSEU2 |
398 | 24176 | |||
| 50S ribosomal protein L1 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplA PE=3 SV=1 | |||||
| RL1_PSEF5 |
398 | 24047 | |||
| 50S ribosomal protein L1 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplA PE=3 SV=1 | |||||
| RL1_PSEFS |
336 | 24061 | 6.1 | ||
| 50S ribosomal protein L1 OS=Pseudomonas fluorescens (strain SBW25) GN=rplA PE=3 SV=1 | |||||
| 1 sameset of RL1_PSEFS | |||||
| RL1_PSEPF |
336 | 24193 | |||
| 50S ribosomal protein L1 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplA PE=3 SV=1 | |||||
| RL1_AZOVD |
304 | 24230 | 6.1 | ||
| 50S ribosomal protein L1 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplA PE=3 SV=1 | |||||
| RL1_PSEMY |
151 | 23957 | 6.1 | ||
| 50S ribosomal protein L1 OS=Pseudomonas mendocina (strain ymp) GN=rplA PE=3 SV=1 | |||||
| 1 sameset of RL1_PSEMY | |||||
| RL1_PSEU5 |
151 | 24129 | |||
| 50S ribosomal protein L1 OS=Pseudomonas stutzeri (strain A1501) GN=rplA PE=3 SV=1 | |||||
| RL1_ACTP2 |
118 | 23932 | 6.1 | ||
| 50S ribosomal protein L1 OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=rplA PE=3 SV=1 | |||||
| 58 samesets of RL1_ACTP2 |
|||||
| RL1_ACIAC |
61 | 23909 | 6.1 | ||
| 50S ribosomal protein L1 OS=Acidovorax avenae subsp. citrulli (strain AAC00-1) GN=rplA PE=3 SV=1 | |||||
| 46 samesets of RL1_ACIAC |
|||||
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OTCC_PSEPK | 445 | Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARCA_PSEPK | 405 | Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DLDH2_PSEPU | 387 | Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DBHB_PSEAE | 318 | DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3 |
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