MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 51–60 (out of 187)


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+51

Accession Score Description
1 IDH_AZOVI 98 Isocitrate dehydrogenase [NADP] OS=Azotobacter vinelandii GN=icd PE=1 SV=5

+52

Accession Score Description
1 RL10_PSEPG 96 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

+53

Accession Score Description
1 OPRI_PSEAE 93 Major outer membrane lipoprotein OS=Pseudomonas aeruginosa GN=oprI PE=3 SV=1

+54

Accession Score Description
1 SYA_PSEPG 92 Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2

+55

Accession Score Description
1 ATPG_PSEPG 92 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1

+56

Accession Score Description
1 RL29_PSE14 91 50S ribosomal protein L29 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmC PE=3 SV=1

+57

Accession Score Description
1 CLPB_PSEPK 90 Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1

-58

Accession Score Description
1 ATPD_PSEPG 89 ATP synthase subunit delta OS=Pseudomonas putida (strain GB-1) GN=atpH PE=3 SV=1
Score Mass Matches Sequences emPAI
58.1 ATPD_PSEPG 89 19242 4 (2) 3 (2) 0.26
ATP synthase subunit delta OS=Pseudomonas putida (strain GB-1) GN=atpH PE=3 SV=1
3 samesets of ATPD_PSEPG
ATPD_PSEPK 89 19242 4 (2) 3 (2) 0.26
ATP synthase subunit delta OS=Pseudomonas putida (strain KT2440) GN=atpH PE=3 SV=1
ATPD_PSEPW 89 19288 4 (2) 3 (2) 0.26
ATP synthase subunit delta OS=Pseudomonas putida (strain W619) GN=atpH PE=3 SV=1
ATPD_PSEP1 89 19242 4 (2) 3 (2) 0.26
ATP synthase subunit delta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpH PE=3 SV=1

-4 peptide matches (3 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
483   351.2021 700.3896 700.3868 4.09 1 55 0.0032 +1Score > 42 indicates identity U R.LGQEVR.L
1362 +1 551.3010 1100.5874 1100.5826 4.41 0 33 0.19 +1Score > 41 indicates identity
Score > 38 indicates homology
R.AGDLVIDGSVR.G
2789   578.0234 1731.0484 1731.0222 15.1 1 65 1.4e-006 +1Score > 25 indicates identity
Score > 19 indicates homology
U R.LLLLPEIAALFDLYK.A

1 subset or intersection (10 subset proteins in total)

Score Mass Subset of
ATPD_PSEE4 55 19158 58.1
ATP synthase subunit delta OS=Pseudomonas entomophila (strain L48) GN=atpH PE=3 SV=1
9 samesets of ATPD_PSEE4
SMC_MYCLE 55 131289
Chromosome partition protein smc OS=Mycobacterium leprae GN=smc PE=3 SV=1
HSPQ_BLOFL 55 12184
Heat shock protein hspQ OS=Blochmannia floridanus GN=hspQ PE=3 SV=1
MEND_SYNJB 55 67856
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase OS=Synechococcus sp. GN=menD PE=3 SV=1
MRAY_THISH 55 39306
Phospho-N-acetylmuramoyl-pentapeptide-transferase OS=Thioalkalivibrio sp. (strain HL-EbGR7) GN=mraY PE=3 SV=1
TDH_SHELP 55 37548
L-threonine 3-dehydrogenase OS=Shewanella loihica (strain ATCC BAA-1088 / PV-4) GN=tdh PE=3 SV=1
DAPF_SHEB2 55 30427
Diaminopimelate epimerase OS=Shewanella baltica (strain OS223) GN=dapF PE=3 SV=1
DAPF_SHEB5 55 30427
Diaminopimelate epimerase OS=Shewanella baltica (strain OS155 / ATCC BAA-1091) GN=dapF PE=3 SV=1
DAPF_SHEB8 55 30427
Diaminopimelate epimerase OS=Shewanella baltica (strain OS185) GN=dapF PE=3 SV=1
DAPF_SHEB9 55 30427
Diaminopimelate epimerase OS=Shewanella baltica (strain OS195) GN=dapF PE=3 SV=1

+59

Accession Score Description
1 ILVC_PSEPG 88 Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1

+60

Accession Score Description
1 RS11_FRATF 88 30S ribosomal protein S11 OS=Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA) GN=rpsK PE=3 SV=1
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