MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 51–60 (out of 187)


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+51

Accession Score Description
1 IDH_AZOVI 98 Isocitrate dehydrogenase [NADP] OS=Azotobacter vinelandii GN=icd PE=1 SV=5

+52

Accession Score Description
1 RL10_PSEPG 96 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

+53

Accession Score Description
1 OPRI_PSEAE 93 Major outer membrane lipoprotein OS=Pseudomonas aeruginosa GN=oprI PE=3 SV=1

+54

Accession Score Description
1 SYA_PSEPG 92 Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2

-55

Accession Score Description
1 ATPG_PSEPG 92 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1
Score Mass Matches Sequences emPAI
55.1 ATPG_PSEPG 92 31653 7 (6) 6 (5) 0.42
ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1
2 samesets of ATPG_PSEPG
ATPG_PSEPK 92 31558 7 (6) 6 (5) 0.42
ATP synthase gamma chain OS=Pseudomonas putida (strain KT2440) GN=atpG PE=3 SV=1
ATPG_PSEP1 92 31558 7 (6) 6 (5) 0.42
ATP synthase gamma chain OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpG PE=3 SV=1

-7 peptide matches (6 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
578   373.7264 745.4382 745.4334 6.51 0 43 0.0064 +1Score > 39 indicates identity
Score > 34 indicates homology
U R.LSVVSNK.F
1210   510.7929 1019.5712 1019.5651 6.02 1 12 1.2 +2Score > 39 indicates identity
Score > 25 indicates homology
K.ELQLIYNK.A
1430 +1 566.2867 1130.5588 1130.5567 1.86 0 43 0.004 +1Score > 41 indicates identity
Score > 31 indicates homology
U K.NATDNAGDLIK.E
2444   525.2438 1572.7096 1572.6997 6.26 0 26 0.017 +1Score > 39 indicates identity
Score > 21 indicates homology
U K.HHWDYLYEPDAK.E
2472   527.9445 1580.8117 1580.8059 3.65 1 37 0.012 +1Score > 40 indicates identity
Score > 30 indicates homology
U R.QVIGHLANANPEYR.H
4291   976.5325 2926.5757 2926.5362 13.5 1 35 0.00059 +1Score > 33 indicates identity
Score > 15 indicates homology
U K.FINTMTQKPTVEQLVPLVATPDQDLK.H + Deamidated (NQ)

5 subsets and intersections (13 subset proteins in total)

Score Mass Subset of
ATPG_PSEE4 66 31602 55.1
ATP synthase gamma chain OS=Pseudomonas entomophila (strain L48) GN=atpG PE=3 SV=1
ATPG_PSEMY 53 31680 55.1
ATP synthase gamma chain OS=Pseudomonas mendocina (strain ymp) GN=atpG PE=3 SV=1
ATPG_PSE14 50 31458 55.1
ATP synthase gamma chain OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpG PE=3 SV=1
5 samesets of ATPG_PSE14
ATPG_PSEFS 50 31488
ATP synthase gamma chain OS=Pseudomonas fluorescens (strain SBW25) GN=atpG PE=3 SV=1
ATPG_PSEPF 50 31518
ATP synthase gamma chain OS=Pseudomonas fluorescens (strain Pf0-1) GN=atpG PE=3 SV=1
ATPG_PSEPW 50 31545
ATP synthase gamma chain OS=Pseudomonas putida (strain W619) GN=atpG PE=3 SV=1
ATPG_PSEU2 50 31458
ATP synthase gamma chain OS=Pseudomonas syringae pv. syringae (strain B728a) GN=atpG PE=3 SV=1
ATPG_PSEF5 50 31532
ATP synthase gamma chain OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=atpG PE=3 SV=1
ATPG_PSESM 42 31429 55.1
ATP synthase gamma chain OS=Pseudomonas syringae pv. tomato GN=atpG PE=3 SV=1
ATPG_PSEA7 38 31704 55.1
ATP synthase gamma chain OS=Pseudomonas aeruginosa (strain PA7) GN=atpG PE=3 SV=1
3 samesets of ATPG_PSEA7
ATPG_PSEAB 38 31704
ATP synthase gamma chain OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=atpG PE=3 SV=1
ATPG_PSEAE 38 31704
ATP synthase gamma chain OS=Pseudomonas aeruginosa GN=atpG PE=3 SV=1
ATPG_PSEA8 38 31704
ATP synthase gamma chain OS=Pseudomonas aeruginosa (strain LESB58) GN=atpG PE=3 SV=1

+56

Accession Score Description
1 RL29_PSE14 91 50S ribosomal protein L29 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmC PE=3 SV=1

+57

Accession Score Description
1 CLPB_PSEPK 90 Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1

+58

Accession Score Description
1 ATPD_PSEPG 89 ATP synthase subunit delta OS=Pseudomonas putida (strain GB-1) GN=atpH PE=3 SV=1

+59

Accession Score Description
1 ILVC_PSEPG 88 Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1

+60

Accession Score Description
1 RS11_FRATF 88 30S ribosomal protein S11 OS=Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA) GN=rpsK PE=3 SV=1
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