MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 51–60 (out of 187)

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+51

Accession Score Description
1 IDH_AZOVI 98 Isocitrate dehydrogenase [NADP] OS=Azotobacter vinelandii GN=icd PE=1 SV=5

-52

Accession Score Description
1 RL10_PSEPG 96 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1
Score Mass Matches Sequences emPAI
52.1 RL10_PSEPG 96 17676 7 (3) 4 (2) 0.28
50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1
3 samesets of RL10_PSEPG
RL10_PSEPK 96 17676 7 (3) 4 (2) 0.28
50S ribosomal protein L10 OS=Pseudomonas putida (strain KT2440) GN=rplJ PE=3 SV=1
RL10_PSEPW 96 17648 7 (3) 4 (2) 0.28
50S ribosomal protein L10 OS=Pseudomonas putida (strain W619) GN=rplJ PE=3 SV=1
RL10_PSEE4 96 17602 5 (3) 3 (2) 0.28
50S ribosomal protein L10 OS=Pseudomonas entomophila (strain L48) GN=rplJ PE=3 SV=1

-7 peptide matches (4 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
684   397.2146 792.4146 792.4130 2.09 1 21 0.13 +1Score > 42 indicates identity
Score > 25 indicates homology		 R.EAGVYVR.V
1310 +1 536.3150 1070.6154 1070.6084 6.59 0 42 0.0016 +1Score > 40 indicates identity
Score > 26 indicates homology		 U K.VALSAVVADAR.G
1443 +1 567.8116 1133.6086 1133.6114 -2.46 0 15 1.3 +2Score > 41 indicates identity
Score > 29 indicates homology		 R.LMSVIQGATSK.L
2738 +1 570.2994 1707.8764 1707.8692 4.17 1 60 1.4e-005 +1Score > 40 indicates identity
Score > 24 indicates homology		 U K.GPTLIAFSNEHPGAAAR.L

1 subset or intersection (7 subset proteins in total)

Score Mass Subset of
RL10_AZOVD 79 17534 52.1
50S ribosomal protein L10 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplJ PE=3 SV=1
6 samesets of RL10_AZOVD
RL10_PSEA7 79 17623
50S ribosomal protein L10 OS=Pseudomonas aeruginosa (strain PA7) GN=rplJ PE=3 SV=1
RL10_PSEA8 79 17623
50S ribosomal protein L10 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplJ PE=3 SV=1
RL10_PSEAB 79 17623
50S ribosomal protein L10 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplJ PE=3 SV=1
RL10_PSEAE 79 17623
50S ribosomal protein L10 OS=Pseudomonas aeruginosa GN=rplJ PE=3 SV=1
RL10_PSEMY 79 17574
50S ribosomal protein L10 OS=Pseudomonas mendocina (strain ymp) GN=rplJ PE=3 SV=1
RL10_PSEU5 79 17514
50S ribosomal protein L10 OS=Pseudomonas stutzeri (strain A1501) GN=rplJ PE=3 SV=1

+53

Accession Score Description
1 OPRI_PSEAE 93 Major outer membrane lipoprotein OS=Pseudomonas aeruginosa GN=oprI PE=3 SV=1

+54

Accession Score Description
1 SYA_PSEPG 92 Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2

+55

Accession Score Description
1 ATPG_PSEPG 92 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1

+56

Accession Score Description
1 RL29_PSE14 91 50S ribosomal protein L29 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmC PE=3 SV=1

+57

Accession Score Description
1 CLPB_PSEPK 90 Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1

+58

Accession Score Description
1 ATPD_PSEPG 89 ATP synthase subunit delta OS=Pseudomonas putida (strain GB-1) GN=atpH PE=3 SV=1

+59

Accession Score Description
1 ILVC_PSEPG 88 Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1

+60

Accession Score Description
1 RS11_FRATF 88 30S ribosomal protein S11 OS=Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA) GN=rpsK PE=3 SV=1
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