MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 41–50 (out of 187)


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+41

Accession Score Description
1 PORF_PSEAE 123 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+42

Accession Score Description
1 GLNA_AZOVI 120 Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1

+43

Accession Score Description
1 YJJK_ECOLI 117 Uncharacterized ABC transporter ATP-binding protein yjjK OS=Escherichia coli (strain K12) GN=yjjK PE=1 SV=2

+44

Accession Score Description
1 RL21_PSEP1 113 50S ribosomal protein L21 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplU PE=3 SV=1

+45

Accession Score Description
1 YEAG_ECOLI 109 Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1

+46

Accession Score Description
1 ODO1_AZOVI 104 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1

+47

Accession Score Description
1 RL25_PSEPK 103 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2

+48

Accession Score Description
1 DHSB_ECOLI 103 Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1

+49

Accession Score Description
1 RL22_PSE14 102 50S ribosomal protein L22 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplV PE=3 SV=1

-50

Accession Score Description
1 RL6_PSEP1 98 50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1
Score Mass Matches Sequences emPAI
50.1 RL6_PSEP1 98 19131 6 (4) 5 (3) 0.42
50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1
6 samesets of RL6_PSEP1
RL6_PSEPG 98 19131 6 (4) 5 (3) 0.42
50S ribosomal protein L6 OS=Pseudomonas putida (strain GB-1) GN=rplF PE=3 SV=1
RL6_PSEPK 98 19131 6 (4) 5 (3) 0.42
50S ribosomal protein L6 OS=Pseudomonas putida (strain KT2440) GN=rplF PE=3 SV=1
RL6_PSEF5 98 19170 5 (4) 4 (3) 0.41
50S ribosomal protein L6 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplF PE=3 SV=1
RL6_PSEFS 98 19185 5 (4) 4 (3) 0.41
50S ribosomal protein L6 OS=Pseudomonas fluorescens (strain SBW25) GN=rplF PE=3 SV=1
RL6_PSEPF 98 19184 5 (4) 4 (3) 0.41
50S ribosomal protein L6 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplF PE=3 SV=1
RL6_PSEPW 98 19115 5 (4) 4 (3) 0.42
50S ribosomal protein L6 OS=Pseudomonas putida (strain W619) GN=rplF PE=3 SV=1

-6 peptide matches (5 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
747   414.7460 827.4774 827.4753 2.64 1 29 0.11 +1Score > 40 indicates identity
Score > 32 indicates homology
K.LPSGVEVK.F
1111 +1 489.2764 976.5382 976.5342 4.17 0 45 0.022 +1Score > 41 indicates identity U K.FAGQQLSVK.G
1367   552.8462 1103.6778 1103.6703 6.86 1 41 0.0048 +1Score > 30 indicates identity U R.KLQLVGVGYK.A
1477   383.5305 1147.5697 1147.5662 3.03 0 15 0.19 +1Score > 42 indicates identity
Score > 20 indicates homology
U R.DFRPPEPYK.G
1565   592.3467 1182.6788 1182.6721 5.74 1 83 1.7e-006 +1Score > 38 indicates identity U K.QLVGQVAAEIR.D

4 subsets and intersections (21 subset proteins in total)

Score Mass Subset of
RL6_PSE14 90 19506 50.1
50S ribosomal protein L6 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplF PE=3 SV=1
8 samesets of RL6_PSE14
RL6_PSESM 90 19266
50S ribosomal protein L6 OS=Pseudomonas syringae pv. tomato GN=rplF PE=3 SV=1
RL6_PSEU2 90 19236
50S ribosomal protein L6 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplF PE=3 SV=1
RL6_PSEA7 90 19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa (strain PA7) GN=rplF PE=3 SV=1
RL6_PSEA8 90 19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplF PE=3 SV=1
RL6_PSEAB 90 19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplF PE=3 SV=1
RL6_PSEAE 90 19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa GN=rplF PE=3 SV=1
RL6_PSEMY 90 19130
50S ribosomal protein L6 OS=Pseudomonas mendocina (strain ymp) GN=rplF PE=3 SV=1
RL6_PSEU5 90 19167
50S ribosomal protein L6 OS=Pseudomonas stutzeri (strain A1501) GN=rplF PE=3 SV=1
RL6_CHRVO 83 18841 50.1
50S ribosomal protein L6 OS=Chromobacterium violaceum GN=rplF PE=3 SV=1
3 samesets of RL6_CHRVO
RL6_SHELP 83 18777
50S ribosomal protein L6 OS=Shewanella loihica (strain ATCC BAA-1088 / PV-4) GN=rplF PE=3 SV=1
RL6_SHESH 83 18751
50S ribosomal protein L6 OS=Shewanella sediminis (strain HAW-EB3) GN=rplF PE=3 SV=1
RL6_SHEWM 83 18852
50S ribosomal protein L6 OS=Shewanella woodyi (strain ATCC 51908 / MS32) GN=rplF PE=3 SV=1
RL6_PSEE4 53 19015 50.1
50S ribosomal protein L6 OS=Pseudomonas entomophila (strain L48) GN=rplF PE=3 SV=1
RL6_ACIB3 41 19085 50.1
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain AB307-0294) GN=rplF PE=3 SV=1
6 samesets of RL6_ACIB3
RL6_ACIB5 41 19085
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain AB0057) GN=rplF PE=3 SV=1
RL6_ACIBS 41 19113
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain SDF) GN=rplF PE=3 SV=1
RL6_ACIBT 41 19085
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain ATCC 17978 / NCDC KC 755) GN=rplF PE=3 SV=2
RL6_ACIBY 41 19085
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain AYE) GN=rplF PE=3 SV=1
RL6_AERHH 41 18568
50S ribosomal protein L6 OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) GN=rplF PE=3 SV=1
RL6_AERS4 41 18556
50S ribosomal protein L6 OS=Aeromonas salmonicida (strain A449) GN=rplF PE=3 SV=1

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