MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 187)

Page: 1 2 3 4 5 6 … 19 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1352 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
4 EFTU_CYAP7 34 Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1
3 EFTU_CARRP 180 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1236 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 1121 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSEFS 949 ATP synthase subunit beta OS=Pseudomonas fluorescens (strain SBW25) GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 954 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 173 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 163 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 667 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
4 CH602_SORC5 69 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
3 CH60_COLMA 82 60 kDa chaperonin OS=Colwellia maris GN=groL PE=3 SV=2
2 CH601_ECOK1 119 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1

-5

Accession Score Description
Family member distances as a dendrogram 1 RPOC_PSEPK 451 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1
4 RPOC_PELUB 108 DNA-directed RNA polymerase subunit beta' OS=Pelagibacter ubique GN=rpoC PE=3 SV=1
3 RPOC_SYNAS 149 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
2 RPOB_PSEP1 316 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoB PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
RPOC_PSEPK 451 155386 33 (22) 24 (15) 0.28
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1
1 sameset of RPOC_PSEPK
RPOC_PSEP1 451 155358 33 (22) 24 (15) 0.28
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoC PE=3 SV=1
RPOB_PSEP1 316 151468 30 (17) 24 (14) 0.27
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoB PE=3 SV=1
3 samesets of RPOB_PSEP1
RPOB_PSEPG 316 151555 30 (17) 24 (14) 0.27
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1
RPOB_PSEPK 316 151468 30 (17) 24 (14) 0.27
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1
RPOB_PSEPW 316 151379 29 (17) 23 (14) 0.27
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain W619) GN=rpoB PE=3 SV=1
RPOC_SYNAS 149 154698 6 (5) 5 (4) 0.06
DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
RPOC_PELUB 108 155438 4 (4) 3 (3) 0.04
DNA-directed RNA polymerase subunit beta' OS=Pelagibacter ubique GN=rpoC PE=3 SV=1

-66 peptide matches (55 non-duplicate, 11 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 Peptide
224   516.3046 515.2973 515.2955 3.57 0 10 9.2 +6Score > 47 indicates identity
Score > 33 indicates homology		 X R.ALAIE.K
522   358.7201 715.4256 715.4228 3.93 0 30 0.19 +1Score > 43 indicates identity
Score > 35 indicates homology		 U X K.TLVDIR.N
565   367.7329 733.4512 733.4486 3.56 0 19 1.4 +3Score > 35 indicates identity
Score > 33 indicates homology		 U X K.VYLAIR.R
573 +1 372.2564 742.4982 742.4953 4.01 0 19 0.32 +1Score > 32 indicates identity
Score > 27 indicates homology		 U X R.VLLGITK.A
700   400.7360 799.4574 799.4552 2.81 1 14 0.46 +1Score > 41 indicates identity
Score > 23 indicates homology		 U X K.VIVEQGR.R
829   430.7473 859.4800 859.4763 4.30 0 12 11 +8Score > 42 indicates identity
Score > 35 indicates homology		 U X R.IVDTTVGR.A
833 +1 430.7506 859.4866 859.4803 7.33 0 38 0.051 +1Score > 41 indicates identity
Score > 37 indicates homology		 U X K.VIDLWSK.A
849   436.2874 870.5602 870.5538 7.37 0 32 0.081 +1Score > 33 indicates identity U X R.LLGVSALAK.Y
874   441.2469 880.4792 880.4766 2.96 1 12 1.2 +2Score > 38 indicates identity
Score > 25 indicates homology		 U X R.HINQLEK.A
947   458.7790 915.5434 915.5389 4.96 1 66 0.00011 +1Score > 39 indicates identity U X X X R.KGLADTALK.T
953   460.7480 919.4814 919.4763 5.58 1 16 1.7 +3Score > 42 indicates identity
Score > 31 indicates homology		 U X R.VADLFEAR.R
966   463.2276 924.4406 924.4413 -0.75 0 23 0.13 +1Score > 40 indicates identity
Score > 27 indicates homology		 X K.NIVDGDHR.M
1045   317.8635 950.5687 950.5661 2.66 0 26 0.35 +1Score > 34 indicates identity X R.VIVSQLHR.S
1047   318.5078 952.5016 952.4978 3.97 0 19 0.51 +1Score > 39 indicates identity
Score > 29 indicates homology		 X K.LNHLVDDK.M
1059   479.2993 956.5840 956.5906 -6.84 1 28 0.58 +2Score > 39 indicates identity U X M.KDLLNLLK.N + Deamidated (NQ)
1064   480.2908 958.5670 958.5600 7.34 0 36 0.014 +1Score > 37 indicates identity
Score > 30 indicates homology		 U X R.GVTFAVPLR.V
1127   494.7890 987.5634 987.5601 3.43 1 30 0.14 +1Score > 39 indicates identity
Score > 34 indicates homology		 U X R.VLTEAAVTGK.R
1127   494.7890 987.5634 987.5600 3.45 1 12 10 +4Score > 39 indicates identity
Score > 34 indicates homology		 U X R.VLTEASLAGK.V
1137   496.7631 991.5116 991.5087 2.99 0 30 0.25 +1Score > 41 indicates identity
Score > 37 indicates homology		 U X K.AQQYIVDR.R
1151   498.8355 995.6564 995.6491 7.36 1 30 0.048 +1Score > 29 indicates identity U X R.KLPASVLLR.A
1161   501.2756 1000.5366 1000.5302 6.49 0 38 0.03 +1Score > 42 indicates identity
Score > 35 indicates homology		 U X R.ADGNLVAVSR.S
1201   339.5150 1015.5232 1015.5199 3.19 0 24 1.4 +2Score > 42 indicates identity
Score > 38 indicates homology		 U X R.TFHIGGAASR.T
1223   514.3008 1026.5870 1026.5822 4.72 0 53 0.002 +1Score > 39 indicates identity
Score > 39 indicates homology		 U X R.VSALGPGGLTR.E
1255   521.8207 1041.6268 1041.6183 8.25 0 38 0.056 +1Score > 38 indicates identity U X R.SVITVGPTLR.L
1275 +1 527.8210 1053.6274 1053.6182 8.75 1 47 0.0027 +1Score > 36 indicates identity
Score > 34 indicates homology		 U X K.LSLELVPQR.L
1289   530.2904 1058.5662 1058.5608 5.17 1 57 0.0018 +1Score > 42 indicates identity U X R.IIGNATDEVK.E
1486 +1 578.3448 1154.6750 1154.6659 7.91 1 63 7.7e-005 +1Score > 37 indicates identity
Score > 34 indicates homology		 U X K.QLIDELVAVR.H
1494 +1 578.8222 1155.6298 1155.6499 -17.4 1 68 8.1e-005 +1Score > 40 indicates identity U X K.QLIDELVAVR.H + Deamidated (NQ)
1549   392.8539 1175.5399 1175.5360 3.30 0 19 0.048 +1Score > 41 indicates identity
Score > 18 indicates homology		 U X R.SPGVFFDHDR.G
1585   596.3035 1190.5924 1190.5932 -0.59 1 35 0.043 +1Score > 41 indicates identity
Score > 33 indicates homology		 U X R.VFADLQEVDR.V
1706   617.8502 1233.6858 1233.6718 11.4 0 45 0.00081 +1Score > 38 indicates identity
Score > 27 indicates homology		 U X K.GTVIDVQVFTR.D
1884 +1 657.8250 1313.6354 1313.6252 7.83 0 52 0.00015 +1Score > 41 indicates identity
Score > 26 indicates homology		 U X X X R.FATSDLNDLYR.R
1915   441.8959 1322.6659 1322.6579 6.04 0 39 0.0011 +1Score > 41 indicates identity
Score > 21 indicates homology		 U X K.GIVDDIDHLGNR.R
1917 +1 662.9038 1323.7930 1323.7762 12.7 0 62 5.1e-005 +1Score > 32 indicates identity U X R.LLDLSAPDIIVR.N
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_6_20250714122635.raw

Score > 33 indicates identity

Score > 26 indicates homology
1940   446.6030 1336.7872 1336.7755 8.77 1 51 0.00018 -1Score > 33 indicates identity
Score > 26 indicates homology		 U X R.YKLPYGAVISVK.E
0.36 0 10 2.1 2 QAVNVPIIGLWK  
-15.5 1 9 2.4 3 LIHDSVLVKSVK  
11.8 1 8 3.4 4 VLHTAAGELLSVK  
6.23 0 8 3.5 5 VLPCVLLPLADK  
11.8 1 8 3.5 5 YTSRITVAIVSK  
11.8 0 7 3.8 7 IVPVGQQAVDALK  
-18.5 0 7 3.8 7 LLGAIPQVVWIK   + Deamidated (NQ)
-8.06 1 7 4.1 9 FGKLVIPHGITR  
-8.06 1 6 5.6 10 IHPIGFRLGVTK  
1974   677.8524 1353.6902 1353.7140 -17.5 0 25 0.021 +2Score > 40 indicates identity
Score > 21 indicates homology		 U X K.LQQGDDLAPGVLK.I + Deamidated (NQ)
2032   693.3745 1384.7344 1384.7310 2.45 0 78 9.8e-007 +1Score > 40 indicates identity
Score > 31 indicates homology		 U X R.SALNGQVVDGGAGLK.K
2103 +1 706.3694 1410.7242 1410.7143 7.04 1 68 0.0001 +1Score > 40 indicates identity U X K.YIVNEIQDVYR.L
2222   738.3582 1474.7018 1474.6728 19.7 1 37 0.0016 +1Score > 41 indicates identity
Score > 21 indicates homology		 U X R.TNQYGFLESPYR.V + Deamidated (NQ)
2244 +1 495.6015 1483.7827 1483.7783 2.94 1 59 6.3e-005 +1Score > 40 indicates identity
Score > 29 indicates homology		 U X R.LIPAGTGLAYHSER.K
2315 +1 505.2852 1512.8338 1512.8260 5.13 1 14 0.49 +1Score > 38 indicates identity
Score > 23 indicates homology		 U X R.SALNGQVVDGGAGLKK.G
2369   514.2977 1539.8713 1539.8523 12.3 1 54 0.001 +1Score > 36 indicates identity U X K.MALELFKPFIFGK.L
2442   393.9612 1571.8157 1571.8096 3.86 1 17 0.037 +1Score > 40 indicates identity
Score > 15 indicates homology		 U X K.WDPHTHPIVTELK.G
2570   812.4274 1622.8402 1622.8152 15.5 0 64 1.4e-005 +1Score > 40 indicates identity
Score > 28 indicates homology		 U X R.STGSYSLVTQQPLGGK.A + Deamidated (NQ)
2731   853.9236 1705.8326 1705.8271 3.23 1 55 0.00034 +1Score > 41 indicates identity
Score > 33 indicates homology		 U X R.GEVISDGPSDPHDILR.L
2732   569.6183 1705.8331 1705.8271 3.48 1 73 4.4e-006 +1Score > 41 indicates identity
Score > 32 indicates homology		 U X R.GEVISDGPSDPHDILR.L
2815   874.4430 1746.8714 1746.8611 5.94 1 62 0.0002 +1Score > 41 indicates identity
Score > 37 indicates homology		 U X K.LADLPESGQMVLFDGR.T
2867 +1 593.3209 1776.9409 1776.9159 14.1 1 56 9.3e-005 +1Score > 39 indicates identity
Score > 28 indicates homology		 U X R.EGLSVLQYFISTHGAR.K
2868   889.4792 1776.9438 1776.9159 15.7 1 28 0.051 +1Score > 39 indicates identity
Score > 27 indicates homology		 U X R.EGLSVLQYFISTHGAR.K
3145   491.7773 1963.0801 1963.0502 15.2 0 32 0.0018 +1Score > 35 indicates identity
Score > 17 indicates homology		 U X R.MGHINLATPVAHIWFLK.S + Oxidation (M)
3249   1017.5062 2032.9978 2032.9589 19.2 1 58 9.6e-005 +1Score > 40 indicates identity
Score > 30 indicates homology		 U X K.ASLSTQSFISAASFQETTR.V + 2 Deamidated (NQ)
3563   736.0552 2205.1438 2205.1106 15.0 1 22 0.029 +1Score > 38 indicates identity
Score > 19 indicates homology		 U X K.SVFPIISYSGNAALEYVGYR.L
3565   736.3857 2206.1353 2206.0946 18.4 1 30 0.0023 +1Score > 38 indicates identity
Score > 16 indicates homology		 U X K.SVFPIISYSGNAALEYVGYR.L + Deamidated (NQ)
3699   767.4252 2299.2538 2299.2286 10.9 0 10 0.13 +1Score > 35 indicates identity
Score > 14 indicates homology		 U X R.ALLFQVVPAGLPYDVVNQPMK.K + Deamidated (NQ)

+43 subsets and intersections (898 subset proteins in total)

+6

Accession Score Description
1 RL1_PSEE4 451 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+7

Accession Score Description
1 OTCC_PSEPK 445 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+8

Accession Score Description
1 ARCA_PSEPK 405 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+9

Accession Score Description
1 DLDH2_PSEPU 387 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+10

Accession Score Description
1 DBHB_PSEAE 318 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3
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