MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 41–50 (out of 187)

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+41

Accession Score Description
1 PORF_PSEAE 123 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+42

Accession Score Description
1 GLNA_AZOVI 120 Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1

+43

Accession Score Description
1 YJJK_ECOLI 117 Uncharacterized ABC transporter ATP-binding protein yjjK OS=Escherichia coli (strain K12) GN=yjjK PE=1 SV=2

-44

Accession Score Description
1 RL21_PSEP1 113 50S ribosomal protein L21 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplU PE=3 SV=1
Score Mass Matches Sequences emPAI
44.1 RL21_PSEP1 113 11624 8 (4) 5 (2) 0.46
50S ribosomal protein L21 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplU PE=3 SV=1
4 samesets of RL21_PSEP1
RL21_PSEPG 113 11624 8 (4) 5 (2) 0.46
50S ribosomal protein L21 OS=Pseudomonas putida (strain GB-1) GN=rplU PE=3 SV=1
RL21_PSEPK 113 11624 8 (4) 5 (2) 0.46
50S ribosomal protein L21 OS=Pseudomonas putida (strain KT2440) GN=rplU PE=3 SV=2
RL21_PSEPW 113 11638 8 (4) 5 (2) 0.46
50S ribosomal protein L21 OS=Pseudomonas putida (strain W619) GN=rplU PE=3 SV=1
RL21_PSEE4 113 11596 7 (4) 4 (2) 0.46
50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1

-8 peptide matches (5 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
291   602.3527 601.3454 601.3435 3.16 0 19 1.2 +2Score > 45 indicates identity
Score > 33 indicates homology		 K.ITGIQA.-
1044 +1 476.2541 950.4936 950.4862 7.88 1 27 1.3 +2Score > 40 indicates identity R.QWFTEIK.I
1143 +1 497.7840 993.5534 993.5495 3.97 0 47 0.00041 +1Score > 38 indicates identity
Score > 25 indicates homology		 U M.SYAVIVTGGK.Q
1257 +1 522.2978 1042.5810 1042.5771 3.77 1 61 0.00022 +1Score > 40 indicates identity
Score > 37 indicates homology		 U K.VVAEVVSQGR.H
1630   606.3564 1210.6982 1210.6921 5.05 1 3 14 +10Score > 36 indicates identity
Score > 27 indicates homology		 U E.EVTIGAPVVAGAK.V

1 subset or intersection (3 subset proteins in total)

Score Mass Subset of
RL21_HAES1 82 11492 44.1
50S ribosomal protein L21 OS=Haemophilus somnus (strain 129Pt) GN=rplU PE=3 SV=1
2 samesets of RL21_HAES1
RL21_PSEMY 82 11605
50S ribosomal protein L21 OS=Pseudomonas mendocina (strain ymp) GN=rplU PE=3 SV=1
RL21_HAES2 82 11492
50S ribosomal protein L21 OS=Haemophilus somnus (strain 2336) GN=rplU PE=3 SV=1

+45

Accession Score Description
1 YEAG_ECOLI 109 Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1

+46

Accession Score Description
1 ODO1_AZOVI 104 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1

+47

Accession Score Description
1 RL25_PSEPK 103 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2

+48

Accession Score Description
1 DHSB_ECOLI 103 Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1

+49

Accession Score Description
1 RL22_PSE14 102 50S ribosomal protein L22 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplV PE=3 SV=1

+50

Accession Score Description
1 RL6_PSEP1 98 50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1
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