MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita6 sp |
| MS data file | : | PRT1270_T-BRSC_6_20250714122635.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:38:56 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,869 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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41| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PORF_PSEAE | 123 | Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1 |
42| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLNA_AZOVI | 120 | Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 42.1 | GLNA_AZOVI |
120 | 51942 | 5 (3) | 3 (2) | 0.14 | |
| Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1 | |||||||
| 1 sameset of GLNA_AZOVI | |||||||
| GLNA_PSEAE |
120 | 52140 | 3 (3) | 2 (2) | 0.14 | ||
| Glutamine synthetase OS=Pseudomonas aeruginosa GN=glnA PE=1 SV=1 | |||||||
5 peptide matches (4 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 489 |
1 |
351.7017 | 701.3888 | 701.3860 | 3.99 | 0 | 31 | 0.081 | 1Score > 42 indicates identity Score > 32 indicates homology |
K.WIDLR.F | |
| 2320 |
757.8690 | 1513.7234 | 1513.7049 | 12.3 | 0 | 51 | 6.1e-005 | 1Score > 40 indicates identity Score > 22 indicates homology |
U | K.ALNGFTNPSTNSYK.R + Deamidated (NQ) | |
| 2766 |
860.9690 | 1719.9234 | 1719.9018 | 12.6 | 1 | 57 | 3.7e-005 | 1Score > 38 indicates identity Score > 25 indicates homology |
U | R.LVPGFEAPVMLAYSAR.N | |
| 2767 |
574.3152 | 1719.9238 | 1719.9018 | 12.8 | 1 | 63 | 2.2e-005 | 1Score > 38 indicates identity Score > 29 indicates homology |
U | R.LVPGFEAPVMLAYSAR.N |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| GLNA_METCA |
93 | 52398 | 42.1 | ||
| Glutamine synthetase OS=Methylococcus capsulatus GN=glnA PE=3 SV=2 | |||||
| 2 samesets of GLNA_METCA | |||||
| GLNA_PROVU |
93 | 52343 | |||
| Glutamine synthetase OS=Proteus vulgaris GN=glnA PE=3 SV=1 | |||||
| GLNA_VIBAL |
93 | 51694 | |||
| Glutamine synthetase OS=Vibrio alginolyticus GN=glnA PE=3 SV=1 | |||||
43| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YJJK_ECOLI | 117 | Uncharacterized ABC transporter ATP-binding protein yjjK OS=Escherichia coli (strain K12) GN=yjjK PE=1 SV=2 |
44| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL21_PSEP1 | 113 | 50S ribosomal protein L21 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplU PE=3 SV=1 |
45| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YEAG_ECOLI | 109 | Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1 |
46| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODO1_AZOVI | 104 | 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1 |
47| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL25_PSEPK | 103 | 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2 |
48| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DHSB_ECOLI | 103 | Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1 |
49| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL22_PSE14 | 102 | 50S ribosomal protein L22 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplV PE=3 SV=1 |
50| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL6_PSEP1 | 98 | 50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1 |
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