MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita6 sp |
| MS data file | : | PRT1270_T-BRSC_6_20250714122635.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:38:56 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,869 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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41| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PORF_PSEAE | 123 | Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 41.1 | PORF_PSEAE |
123 | 37844 | 16 (7) | 4 (2) | 0.19 | |
| Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1 | |||||||
16 peptide matches (7 non-duplicate, 9 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 407 |
2 |
336.1562 | 670.2978 | 670.2962 | 2.39 | 0 | 22 | 0.21 | 1Score > 37 indicates identity Score > 28 indicates homology |
K.FDFDK.S | |
| 411 |
1 |
671.3057 | 670.2984 | 670.2962 | 3.24 | 0 | 27 | 0.32 | 1Score > 37 indicates identity Score > 34 indicates homology |
K.FDFDK.S | |
| 492 |
1 |
702.4045 | 701.3972 | 701.3959 | 1.82 | 0 | 42 | 0.073 | 1Score > 43 indicates identity |
U | R.VQLDVK.F + Deamidated (NQ) |
| 493 |
351.7061 | 701.3976 | 701.3959 | 2.42 | 0 | 28 | 0.9 | 1Score > 43 indicates identity Score > 40 indicates homology |
U | R.VQLDVK.F + Deamidated (NQ) | |
| 762 |
838.4156 | 837.4083 | 837.4055 | 3.42 | 0 | 43 | 0.03 | 1Score > 40 indicates identity |
U | K.NLADFMK.Q | |
| 763 |
2 |
419.7118 | 837.4090 | 837.4055 | 4.29 | 0 | 38 | 0.014 | 1Score > 40 indicates identity Score > 32 indicates homology |
U | K.NLADFMK.Q |
| 1977 |
3 |
452.2390 | 1353.6952 | 1353.6816 | 10.00 | 1 | 63 | 2.4e-005 | 1Score > 40 indicates identity Score > 30 indicates homology |
U | R.VQLDVKFDFDK.S + Deamidated (NQ) |
42| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLNA_AZOVI | 120 | Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1 |
43| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YJJK_ECOLI | 117 | Uncharacterized ABC transporter ATP-binding protein yjjK OS=Escherichia coli (strain K12) GN=yjjK PE=1 SV=2 |
44| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL21_PSEP1 | 113 | 50S ribosomal protein L21 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplU PE=3 SV=1 |
45| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YEAG_ECOLI | 109 | Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1 |
46| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODO1_AZOVI | 104 | 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1 |
47| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL25_PSEPK | 103 | 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2 |
48| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DHSB_ECOLI | 103 | Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1 |
49| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL22_PSE14 | 102 | 50S ribosomal protein L22 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplV PE=3 SV=1 |
50| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL6_PSEP1 | 98 | 50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1 |
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