MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 187)

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+31

Accession Score Description
1 DNAK_PSEPK 141 Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1

+32

Accession Score Description
1 LEU1_PSEP1 139 2-isopropylmalate synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=leuA PE=3 SV=1

+33

Accession Score Description
1 ODP2_PSEAE 132 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2

+34

Accession Score Description
1 RISB_PSEPG 129 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1

+35

Accession Score Description
1 AMPA_PSEPK 129 Probable cytosol aminopeptidase OS=Pseudomonas putida (strain KT2440) GN=pepA PE=3 SV=1

+36

Accession Score Description
1 PNP_PSEPG 128 Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain GB-1) GN=pnp PE=3 SV=1

+37

Accession Score Description
1 HTPG_PSEFS 127 Chaperone protein htpG OS=Pseudomonas fluorescens (strain SBW25) GN=htpG PE=3 SV=1

+38

Accession Score Description
1 IF2_PSEE4 126 Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1

+39

Accession Score Description
1 ACP_PSEPK 126 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1

-40

Accession Score Description
1 ACON2_PSEAE 125 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1
Score Mass Matches Sequences emPAI
40.1 ACON2_PSEAE 125 94196 11 (5) 7 (4) 0.10
Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

-11 peptide matches (8 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
339   626.3887 625.3814 625.3799 2.42 0 11 0.51 +1Score > 32 indicates identity
Score > 21 indicates homology		 U K.IPVVQA.-
628 +1 384.2625 766.5104 766.5065 5.15 0 34 0.011 +1Score > 27 indicates identity U R.IPLIVGR.G
968 +1 463.2817 924.5488 924.5433 6.03 0 31 0.083 +1Score > 36 indicates identity
Score > 33 indicates homology		 U R.LWLAPPTK.M
1733 +1 622.8321 1243.6496 1243.6449 3.86 1 42 0.0016 +1Score > 41 indicates identity
Score > 27 indicates homology		 U R.VPPGVDEAAYVK.A
1914   661.8512 1321.6878 1321.6838 3.07 0 67 1.3e-005 +1Score > 41 indicates identity
Score > 30 indicates homology		 U R.VQTGSTVVSTSTR.N
2045   694.8546 1387.6946 1387.6772 12.6 1 59 6.5e-005 +1Score > 41 indicates identity
Score > 30 indicates homology		 U R.YLSFDQIAEFR.E
3113   484.2698 1933.0501 1933.0282 11.3 0 10 0.35 +1Score > 36 indicates identity
Score > 18 indicates homology		 U R.GGVSLRPGDGIIHSWLNR.M
3114   645.3591 1933.0555 1933.0282 14.1 0 14 0.24 +1Score > 36 indicates identity
Score > 20 indicates homology		 U R.GGVSLRPGDGIIHSWLNR.M

2 subsets and intersections (4 subset proteins in total)

Score Mass Subset of
ACON2_ECOLI 50 94009 40.1
Aconitate hydratase 2 OS=Escherichia coli (strain K12) GN=acnB PE=1 SV=3
1 sameset of ACON2_ECOLI
ACON2_SYNY3 50 94290
Aconitate hydratase 2 OS=Synechocystis sp. (strain PCC 6803) GN=acnB PE=3 SV=1
BXL7_ARATH 34 84751 40.1
Probable beta-D-xylosidase 7 OS=Arabidopsis thaliana GN=BXL7 PE=2 SV=2
1 sameset of BXL7_ARATH
RIBA_BAUCH 34 22461
GTP cyclohydrolase-2 OS=Baumannia cicadellinicola subsp. Homalodisca coagulata GN=ribA PE=3 SV=1
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