MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 187)

Page: 1 2 3 4 5 6 … 19 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1352 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
4 EFTU_CYAP7 34 Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1
3 EFTU_CARRP 180 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1236 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 1121 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSEFS 949 ATP synthase subunit beta OS=Pseudomonas fluorescens (strain SBW25) GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 954 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 173 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 163 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

-4

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 667 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
4 CH602_SORC5 69 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
3 CH60_COLMA 82 60 kDa chaperonin OS=Colwellia maris GN=groL PE=3 SV=2
2 CH601_ECOK1 119 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
4.1 CH60_PSEPK 667 56765 59 (28) 21 (13) 1.04
60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
4.2 CH601_ECOK1 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1
-27 samesets of CH601_ECOK1
CH60_ENTS8 119 57392 24 (8) 11 (4) 0.26
60 kDa chaperonin OS=Enterobacter sakazakii (strain ATCC BAA-894) GN=groL PE=3 SV=1
CH60_ECO24 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=groL PE=3 SV=1
CH60_ECO27 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=groL PE=3 SV=1
CH60_ECO45 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli O45:K1 (strain S88 / ExPEC) GN=groL PE=3 SV=1
CH60_ECO55 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli (strain 55989 / EAEC) GN=groL PE=3 SV=1
CH60_ECO57 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli O157:H7 GN=groL PE=3 SV=2
CH60_ECO5E 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=groL PE=3 SV=1
CH60_ECO7I 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=groL PE=3 SV=1
CH60_ECO81 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli O81 (strain ED1a) GN=groL PE=3 SV=1
CH60_ECO8A 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli O8 (strain IAI1) GN=groL PE=3 SV=1
CH60_ECOBW 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli (strain K12 / BW2952) GN=groL PE=3 SV=1
CH60_ECODH 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli (strain K12 / DH10B) GN=groL PE=3 SV=1
CH60_ECOHS 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli O9:H4 (strain HS) GN=groL PE=3 SV=1
CH60_ECOL5 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli O6:K15:H31 (strain 536 / UPEC) GN=groL PE=3 SV=1
CH60_ECOL6 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli O6 GN=groL PE=3 SV=2
CH60_ECOLC 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) GN=groL PE=3 SV=1
CH60_ECOLI 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2
CH60_ECOLU 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) GN=groL PE=3 SV=1
CH60_ECOSE 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli (strain SE11) GN=groL PE=3 SV=1
CH60_ECOSM 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=groL PE=3 SV=1
CH60_ECOUT 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Escherichia coli (strain UTI89 / UPEC) GN=groL PE=1 SV=1
CH60_SHIB3 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) GN=groL PE=3 SV=1
CH60_SHIBS 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Shigella boydii serotype 4 (strain Sb227) GN=groL PE=3 SV=1
CH60_SHIDS 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=groL PE=3 SV=1
CH60_SHIF8 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Shigella flexneri serotype 5b (strain 8401) GN=groL PE=3 SV=1
CH60_SHIFL 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Shigella flexneri GN=groL PE=3 SV=2
CH60_SHISS 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin OS=Shigella sonnei (strain Ss046) GN=groL PE=3 SV=1
4.3 CH60_COLMA 82 57711 16 (6) 7 (3) 0.21
60 kDa chaperonin OS=Colwellia maris GN=groL PE=3 SV=2
4.4 CH602_SORC5 69 58067 6 (4) 3 (2) 0.12
60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
1 sameset of CH602_SORC5
CH60_GEOLS 69 58655 10 (4) 4 (2) 0.12
60 kDa chaperonin OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=groL PE=3 SV=1

+68 peptide matches (36 non-duplicate, 32 duplicate)

+42 subsets and intersections (597 subset proteins in total)

+5

Accession Score Description
Family member distances as a dendrogram 1 RPOC_PSEPK 451 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1
4 RPOC_PELUB 108 DNA-directed RNA polymerase subunit beta' OS=Pelagibacter ubique GN=rpoC PE=3 SV=1
3 RPOC_SYNAS 149 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
2 RPOB_PSEP1 316 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoB PE=3 SV=1

+6

Accession Score Description
1 RL1_PSEE4 451 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+7

Accession Score Description
1 OTCC_PSEPK 445 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+8

Accession Score Description
1 ARCA_PSEPK 405 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+9

Accession Score Description
1 DLDH2_PSEPU 387 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+10

Accession Score Description
1 DBHB_PSEAE 318 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3
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