MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 187)

Page: 1 2 3 4 5 6 … 19 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1352 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
4 EFTU_CYAP7 34 Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1
3 EFTU_CARRP 180 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1236 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 1121 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSEFS 949 ATP synthase subunit beta OS=Pseudomonas fluorescens (strain SBW25) GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 954 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 173 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 163 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

-4

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 667 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
4 CH602_SORC5 69 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
3 CH60_COLMA 82 60 kDa chaperonin OS=Colwellia maris GN=groL PE=3 SV=2
2 CH601_ECOK1 119 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
CH60_PSEPK 667 56765 59 (28) 21 (13) 1.04
60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
CH601_ECOK1 119 57464 23 (8) 10 (4) 0.26
60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1
+27 samesets of CH601_ECOK1
CH60_COLMA 82 57711 16 (6) 7 (3) 0.21
60 kDa chaperonin OS=Colwellia maris GN=groL PE=3 SV=2
CH602_SORC5 69 58067 6 (4) 3 (2) 0.12
60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
1 sameset of CH602_SORC5
CH60_GEOLS 69 58655 10 (4) 4 (2) 0.12
60 kDa chaperonin OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=groL PE=3 SV=1

-68 peptide matches (36 non-duplicate, 32 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 Peptide
200 +1 487.3276 486.3203 486.3166 7.71 0 32 0.73 +1Score > 46 indicates identity
Score > 43 indicates homology		 X X X K.VAAVK.A
253   559.3837 558.3764 558.3741 4.19 0 28 0.072 +1Score > 34 indicates identity
Score > 29 indicates homology		 X R.VILSK.E
269 +1 586.3575 585.3502 585.3486 2.77 0 35 0.13 +1Score > 39 indicates identity X K.ATLGPK.G
276 +1 588.3374 587.3301 587.3279 3.87 0 49 0.019 +1Score > 44 indicates identity U X R.AADAIK.D
296 +1 301.6855 601.3564 601.3547 2.85 0 23 1.4 +4Score > 43 indicates identity
Score > 37 indicates homology		 X X X K.ISNIR.E
369 +1 322.2113 642.4080 642.4064 2.49 0 23 0.16 +1Score > 42 indicates identity
Score > 28 indicates homology		 U X R.NVVLAK.S
371 +1 643.4158 642.4085 642.4064 3.23 0 42 0.048 +2Score > 41 indicates identity U X R.NVVLAK.S
381 +1 326.6570 651.2994 651.2976 2.78 0 32 0.047 +1Score > 40 indicates identity
Score > 32 indicates homology		 U X K.FGDSAR.K
435 +2 338.1875 674.3604 674.3599 0.80 0 22 1.9 +7Score > 43 indicates identity
Score > 37 indicates homology		 X K.DGVSVAK.E
436   675.3689 674.3616 674.3599 2.54 0 42 0.077 +1Score > 43 indicates identity X K.DGVSVAK.E
526 +1 360.1786 718.3426 718.3398 3.90 0 24 0.37 +1Score > 43 indicates identity
Score > 33 indicates homology		 X X X X K.APGFGDR.R
554   365.7342 729.4538 729.4497 5.70 1 20 1.3 +3Score > 36 indicates identity
Score > 34 indicates homology		 X X X K.KISNIR.E
633 +1 386.2227 770.4308 770.4286 2.87 1 30 0.16 +2Score > 39 indicates identity
Score > 34 indicates homology		 U X R.AVESPLR.Q
634   771.4382 770.4309 770.4286 2.97 1 26 0.98 +1Score > 39 indicates identity
Score > 38 indicates homology		 U X R.AVESPLR.Q
742 +2 414.2727 826.5308 826.5276 3.89 0 38 0.038 +1Score > 36 indicates identity U X X X K.LAGGVAVIK  
745 +1 827.5384 826.5311 826.5276 4.22 0 45 0.0077 +1Score > 36 indicates identity U X X X K.LAGGVAVIK  
956   921.5073 920.5000 920.4967 3.57 0 61 0.00053 +1Score > 40 indicates identity U X X K.SFGAPTITK.D
957 +2 461.2574 920.5002 920.4967 3.82 0 26 0.19 +1Score > 40 indicates identity
Score > 32 indicates homology		 U X X K.SFGAPTITK.D
974   927.5943 926.5870 926.5800 7.55 0 65 4.5e-005 +1Score > 34 indicates identity U X R.ALAAIIDLK.G
975 +3 464.3017 926.5888 926.5800 9.51 0 57 0.0003 +1Score > 34 indicates identity U X R.ALAAIIDLK.G
1097   972.5745 971.5672 971.5651 2.16 1 66 0.00012 +1Score > 40 indicates identity U X K.ATAAVVAELK.N
1098 +3 486.7917 971.5688 971.5651 3.83 1 57 0.00055 +1Score > 40 indicates identity
Score > 37 indicates homology		 U X K.ATAAVVAELK.N
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_6_20250714122635.raw

Score > 40 indicates identity
1181   337.8463 1010.5171 1010.5145 2.55 1 42 0.031 -1Score > 40 indicates identity U X X X X R.VEDALHATR.A
2.55 0 42 0.031 1 VQDALHATR   + Deamidated (NQ)
2.55 1 30 0.52 3 VEDAIHTAR  
-11.2 0 15 17 4 LDVSLAPAPE  
13.7 1 14 19 5 DLVAEHQAK   + Deamidated (NQ)
2.55 1 9 59 6 GAKSYTGATR  
-4.77 1 8 82 7 KGAVQFSMK   + Oxidation (M)
-11.2 1 8 88 8 KVDEPVVPE  
2.58 1 7 96 9 KNINGHQAK   + 2 Deamidated (NQ)
2.58 1 7 96 9 KNINGHQAK   + 2 Deamidated (NQ)
1182   506.2664 1010.5182 1010.5145 3.71 1 64 0.00022 +1Score > 40 indicates identity U X X X X R.VEDALHATR.A
1913   661.8296 1321.6446 1321.6336 8.34 1 32 0.018 +1Score > 41 indicates identity
Score > 27 indicates homology		 U X K.DAFENMGAQLVK.E
1924 +2 664.8908 1327.7670 1327.7534 10.3 0 67 3e-005 +1Score > 36 indicates identity
Score > 34 indicates homology		 U X K.MLVGVNVLADAVK.A
2033   693.4005 1384.7864 1384.7860 0.29 1 16 0.14 +1Score > 37 indicates identity
Score > 20 indicates homology		 U X K.MLRGVNVLADAVK.V
2110 +1 707.3119 1412.6092 1412.6055 2.62 1 41 0.024 +1Score > 38 indicates identity U X R.AQIENTTSDYDR.E + Deamidated (NQ)
2307 +1 757.3901 1512.7656 1512.7532 8.22 1 100 4.5e-008 +1Score > 40 indicates identity
Score > 39 indicates homology		 U X K.GDNEDQNVGIALLR.R
2308   505.2631 1512.7675 1512.7532 9.42 1 28 0.11 +1Score > 40 indicates identity
Score > 31 indicates homology		 U X K.GDNEDQNVGIALLR.R
2321   757.8901 1513.7656 1513.7372 18.8 1 94 2.6e-007 +1Score > 40 indicates identity U X K.GDNEDQNVGIALLR.R + Deamidated (NQ)
2465   790.9648 1579.9150 1579.9297 -9.30 1 12 8.2 +4Score > 33 indicates identity X R.GVNVLADAVKVTLGPK.G
2548 +2 807.9019 1613.7892 1613.7897 -0.26 0 72 6e-007 +1Score > 41 indicates identity
Score > 22 indicates homology		 U X R.QITANAGDEPSVVADK.V
2969   614.6606 1840.9600 1840.9530 3.76 1 52 4e-005 +1Score > 39 indicates identity
Score > 21 indicates homology		 U X R.QITANAGDEPSVVADKVK.Q
3831 +2 801.4214 2401.2424 2401.1973 18.8 1 67 6.8e-005 +1Score > 37 indicates identity U X K.ANDAAGDGTTTATVLAQAIVNEGLK.A + Deamidated (NQ)
3831 +2 801.4214 2401.2424 2401.2336 3.64 1 55 0.00085 +3Score > 37 indicates identity U X K.ANDAAGDGTTTATVLAQAIITEGLK.A

+42 subsets and intersections (597 subset proteins in total)

+5

Accession Score Description
Family member distances as a dendrogram 1 RPOC_PSEPK 451 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1
4 RPOC_PELUB 108 DNA-directed RNA polymerase subunit beta' OS=Pelagibacter ubique GN=rpoC PE=3 SV=1
3 RPOC_SYNAS 149 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
2 RPOB_PSEP1 316 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoB PE=3 SV=1

+6

Accession Score Description
1 RL1_PSEE4 451 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+7

Accession Score Description
1 OTCC_PSEPK 445 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+8

Accession Score Description
1 ARCA_PSEPK 405 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+9

Accession Score Description
1 DLDH2_PSEPU 387 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+10

Accession Score Description
1 DBHB_PSEAE 318 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3
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