MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 187)


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+31

Accession Score Description
1 DNAK_PSEPK 141 Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1

+32

Accession Score Description
1 LEU1_PSEP1 139 2-isopropylmalate synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=leuA PE=3 SV=1

+33

Accession Score Description
1 ODP2_PSEAE 132 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2

+34

Accession Score Description
1 RISB_PSEPG 129 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1

+35

Accession Score Description
1 AMPA_PSEPK 129 Probable cytosol aminopeptidase OS=Pseudomonas putida (strain KT2440) GN=pepA PE=3 SV=1

+36

Accession Score Description
1 PNP_PSEPG 128 Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain GB-1) GN=pnp PE=3 SV=1

+37

Accession Score Description
1 HTPG_PSEFS 127 Chaperone protein htpG OS=Pseudomonas fluorescens (strain SBW25) GN=htpG PE=3 SV=1

-38

Accession Score Description
1 IF2_PSEE4 126 Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1
Score Mass Matches Sequences emPAI
38.1 IF2_PSEE4 126 91973 3 (2) 3 (2) 0.05
Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1
4 samesets of IF2_PSEE4
IF2_PSEP1 126 91620 3 (2) 3 (2) 0.05
Translation initiation factor IF-2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=infB PE=3 SV=1
IF2_PSEPG 126 91294 3 (2) 3 (2) 0.05
Translation initiation factor IF-2 OS=Pseudomonas putida (strain GB-1) GN=infB PE=3 SV=1
IF2_PSEPK 126 91576 3 (2) 3 (2) 0.05
Translation initiation factor IF-2 OS=Pseudomonas putida (strain KT2440) GN=infB PE=3 SV=1
IF2_PSEPW 126 91289 3 (2) 3 (2) 0.05
Translation initiation factor IF-2 OS=Pseudomonas putida (strain W619) GN=infB PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1113   490.7791 979.5436 979.5338 10.0 0 34 0.064 +1Score > 38 indicates identity
Score > 35 indicates homology
K.TSLLDYIR.R
2160   720.4225 1438.8304 1438.8144 11.2 0 84 7.7e-007 +1Score > 36 indicates identity U R.GPVATILVQDGTLR.Q
4282   973.5333 2917.5781 2917.5298 16.6 1 76 2.5e-006 +1Score > 33 indicates identity U R.VIGGGVGGITESDANLALASNAVLFGFNVR.A

1 subset or intersection (13 subset proteins in total)

Score Mass Subset of
IF2_CAMHC 84 100767 38.1
Translation initiation factor IF-2 OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) GN=infB PE=3 SV=1
12 samesets of IF2_CAMHC
IF2_CAMLR 84 96725
Translation initiation factor IF-2 OS=Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060) GN=infB PE=3 SV=1
IF2_SYNE7 84 110858
Translation initiation factor IF-2 OS=Synechococcus elongatus (strain PCC 7942) GN=infB PE=3 SV=1
IF2_SYNP6 84 110787
Translation initiation factor IF-2 OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=infB PE=3 SV=1
IF2_MICAN 84 109614
Translation initiation factor IF-2 OS=Microcystis aeruginosa (strain NIES-843) GN=infB PE=3 SV=1
IF2_SYNS3 84 118793
Translation initiation factor IF-2 OS=Synechococcus sp. (strain CC9311) GN=infB PE=3 SV=1
IF2_SYNJA 84 111852
Translation initiation factor IF-2 OS=Synechococcus sp. (strain JA-3-3Ab) GN=infB PE=3 SV=1
IF2_CYAP4 84 108559
Translation initiation factor IF-2 OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) GN=infB PE=3 SV=1
IF2_CYAP7 84 121749
Translation initiation factor IF-2 OS=Cyanothece sp. (strain PCC 7424) GN=infB PE=3 SV=1
IF2_SYNJB 84 113485
Translation initiation factor IF-2 OS=Synechococcus sp. GN=infB PE=3 SV=1
IF2_SYNP2 84 105884
Translation initiation factor IF-2 OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) GN=infB PE=3 SV=1
IF2_SYNPW 84 119022
Translation initiation factor IF-2 OS=Synechococcus sp. (strain WH7803) GN=infB PE=3 SV=1
IF2_THEEB 84 104469
Translation initiation factor IF-2 OS=Thermosynechococcus elongatus (strain BP-1) GN=infB PE=3 SV=1

+39

Accession Score Description
1 ACP_PSEPK 126 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1

+40

Accession Score Description
1 ACON2_PSEAE 125 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1
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