MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita6 sp |
| MS data file | : | PRT1270_T-BRSC_6_20250714122635.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:38:56 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,869 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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31| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_PSEPK | 141 | Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1 |
32| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LEU1_PSEP1 | 139 | 2-isopropylmalate synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=leuA PE=3 SV=1 |
33| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODP2_PSEAE | 132 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2 |
34| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RISB_PSEPG | 129 | 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1 |
35| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMPA_PSEPK | 129 | Probable cytosol aminopeptidase OS=Pseudomonas putida (strain KT2440) GN=pepA PE=3 SV=1 |
36| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PNP_PSEPG | 128 | Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain GB-1) GN=pnp PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 36.1 | PNP_PSEPG |
128 | 75207 | 12 (5) | 9 (4) | 0.16 | |
| Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain GB-1) GN=pnp PE=3 SV=1 | |||||||
| 4 samesets of PNP_PSEPG | |||||||
| PNP_PSEE4 |
128 | 75140 | 11 (5) | 8 (4) | 0.16 | ||
| Polyribonucleotide nucleotidyltransferase OS=Pseudomonas entomophila (strain L48) GN=pnp PE=3 SV=1 | |||||||
| PNP_PSEPK |
128 | 75192 | 10 (5) | 7 (4) | 0.16 | ||
| Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain KT2440) GN=pnp PE=3 SV=1 | |||||||
| PNP_PSEPU |
128 | 75166 | 10 (5) | 7 (4) | 0.16 | ||
| Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida GN=pnp PE=3 SV=1 | |||||||
| PNP_PSEP1 |
128 | 75139 | 10 (5) | 7 (4) | 0.16 | ||
| Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=pnp PE=3 SV=1 | |||||||
12 peptide matches (11 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 263 |
574.3348 | 573.3275 | 573.3234 | 7.15 | 1 | 12 | 4.8 | 8Score > 42 indicates identity Score > 32 indicates homology |
E.AAKQR.I + Deamidated (NQ) | ||
| 624 |
383.2196 | 764.4246 | 764.4221 | 3.31 | 0 | 18 | 0.31 | 1Score > 39 indicates identity Score > 25 indicates homology |
K.IPGGFFK.R | ||
| 1769 |
631.8125 | 1261.6104 | 1261.6038 | 5.30 | 1 | 38 | 0.013 | 1Score > 42 indicates identity Score > 31 indicates homology |
U | K.ASIDIEDDGSIK.I | |
| 2181 |
484.5867 | 1450.7383 | 1450.7245 | 9.49 | 1 | 12 | 1.3 | 2Score > 41 indicates identity Score > 26 indicates homology |
U | K.GFFPLSVHYQEK.T | |
| 2182 |
726.3776 | 1450.7406 | 1450.7245 | 11.1 | 1 | 9 | 0.33 | 1Score > 41 indicates identity Score > 17 indicates homology |
U | K.GFFPLSVHYQEK.T | |
| 2248 |
743.9196 | 1485.8246 | 1485.8151 | 6.42 | 1 | 72 | 2e-005 | 1Score > 38 indicates identity |
U | R.GETQALVVATLGTAR.D | |
| 2252 |
745.4354 | 1488.8562 | 1488.8341 | 14.9 | 0 | 13 | 0.18 | 1Score > 34 indicates identity Score > 18 indicates homology |
R.IVDFGAFVNILPGK.D | ||
| 2357 |
767.9770 | 1533.9394 | 1533.9243 | 9.90 | 1 | 30 | 0.017 | 1Score > 25 indicates identity |
U | K.TVRPLNIEVGVLPK.T | |
| 2358 |
1 |
512.3205 | 1533.9397 | 1533.9243 | 10.1 | 1 | 34 | 0.0068 | 1Score > 25 indicates identity |
U | K.TVRPLNIEVGVLPK.T |
| 2674 |
836.4261 | 1670.8376 | 1670.8264 | 6.71 | 1 | 69 | 2e-006 | 1Score > 40 indicates identity Score > 25 indicates homology |
U | K.TFQFGQSTVTLETGR.I | |
| 2728 |
568.6267 | 1702.8583 | 1702.8559 | 1.37 | 0 | 4 | 1.9 | 7Score > 40 indicates identity Score > 19 indicates homology |
U | R.LNILGQMNQIIGQSR.T + 3 Deamidated (NQ); Oxidation (M) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| PNP_AZOVD |
116 | 75912 | 36.1 | ||
| Polyribonucleotide nucleotidyltransferase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=pnp PE=3 SV=1 | |||||
| PNP_PSE14 |
85 | 75121 | 36.1 | ||
| Polyribonucleotide nucleotidyltransferase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=pnp PE=3 SV=1 | |||||
| 6 samesets of PNP_PSE14 | |||||
| PNP_PSEF5 |
85 | 75343 | |||
| Polyribonucleotide nucleotidyltransferase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=pnp PE=3 SV=1 | |||||
| PNP_PSEFS |
85 | 75117 | |||
| Polyribonucleotide nucleotidyltransferase OS=Pseudomonas fluorescens (strain SBW25) GN=pnp PE=3 SV=1 | |||||
| PNP_PSEPF |
85 | 75026 | |||
| Polyribonucleotide nucleotidyltransferase OS=Pseudomonas fluorescens (strain Pf0-1) GN=pnp PE=3 SV=1 | |||||
| PNP_PSESM |
85 | 75108 | |||
| Polyribonucleotide nucleotidyltransferase OS=Pseudomonas syringae pv. tomato GN=pnp PE=3 SV=1 | |||||
| PNP_PSEU2 |
85 | 75123 | |||
| Polyribonucleotide nucleotidyltransferase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=pnp PE=3 SV=1 | |||||
| PNP_PSEPW |
85 | 75250 | |||
| Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain W619) GN=pnp PE=3 SV=1 | |||||
| PNP_PSEMY |
75 | 75677 | 36.1 | ||
| Polyribonucleotide nucleotidyltransferase OS=Pseudomonas mendocina (strain ymp) GN=pnp PE=3 SV=2 | |||||
| 1 sameset of PNP_PSEMY | |||||
| PNP_PSEU5 |
75 | 75420 | |||
| Polyribonucleotide nucleotidyltransferase OS=Pseudomonas stutzeri (strain A1501) GN=pnp PE=3 SV=1 | |||||
| PNP_POLNS |
72 | 77289 | 36.1 | ||
| Polyribonucleotide nucleotidyltransferase OS=Polynucleobacter necessarius (strain STIR1) GN=pnp PE=3 SV=1 | |||||
| 5 samesets of PNP_POLNS | |||||
| PNP_PSEA7 |
72 | 75691 | |||
| Polyribonucleotide nucleotidyltransferase OS=Pseudomonas aeruginosa (strain PA7) GN=pnp PE=3 SV=1 | |||||
| PNP_PSEAB |
72 | 75634 | |||
| Polyribonucleotide nucleotidyltransferase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=pnp PE=3 SV=1 | |||||
| PNP_PSEAE |
72 | 75634 | |||
| Polyribonucleotide nucleotidyltransferase OS=Pseudomonas aeruginosa GN=pnp PE=3 SV=1 | |||||
| PNP_PSEA8 |
72 | 75634 | |||
| Polyribonucleotide nucleotidyltransferase OS=Pseudomonas aeruginosa (strain LESB58) GN=pnp PE=3 SV=1 | |||||
| PNP_POLSQ |
72 | 77293 | |||
| Polyribonucleotide nucleotidyltransferase OS=Polynucleobacter sp. (strain QLW-P1DMWA-1) GN=pnp PE=3 SV=1 | |||||
| PNP_TERTT |
30 | 76334 | 36.1 | ||
| Polyribonucleotide nucleotidyltransferase OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=pnp PE=3 SV=1 | |||||
37| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HTPG_PSEFS | 127 | Chaperone protein htpG OS=Pseudomonas fluorescens (strain SBW25) GN=htpG PE=3 SV=1 |
38| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IF2_PSEE4 | 126 | Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1 |
39| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACP_PSEPK | 126 | Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1 |
40| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACON2_PSEAE | 125 | Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1 |
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