MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Protein families 31–40 (out of 187)

Page: Previous 1 2 3 4 5 6 7 8 9 … 19 Next 

+31

Accession Score Description
1 DNAK_PSEPK 141 Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1

+32

Accession Score Description
1 LEU1_PSEP1 139 2-isopropylmalate synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=leuA PE=3 SV=1

+33

Accession Score Description
1 ODP2_PSEAE 132 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2

+34

Accession Score Description
1 RISB_PSEPG 129 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1

-35

Accession Score Description
1 AMPA_PSEPK 129 Probable cytosol aminopeptidase OS=Pseudomonas putida (strain KT2440) GN=pepA PE=3 SV=1
Score Mass Matches Sequences emPAI
35.1 AMPA_PSEPK 129 52857 9 (4) 7 (3) 0.14
Probable cytosol aminopeptidase OS=Pseudomonas putida (strain KT2440) GN=pepA PE=3 SV=1
1 sameset of AMPA_PSEPK
AMPA_PSEPG 129 52694 8 (4) 6 (3) 0.14
Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1

-9 peptide matches (7 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
561   366.7177 731.4208 731.4177 4.25 0 19 1.4 +3Score > 41 indicates identity
Score > 33 indicates homology		 K.SVAAASVK.T
1365   552.8276 1103.6406 1103.6339 6.13 0 24 0.031 +1Score > 37 indicates identity
Score > 22 indicates homology		 U R.LIVLNYQGGK.K
1612   401.2500 1200.7282 1200.7230 4.28 1 28 0.12 +1Score > 32 indicates identity
Score > 31 indicates homology		 U K.KADKPFVLVGK.G
1783 +1 635.3660 1268.7174 1268.7088 6.78 1 44 0.0056 +1Score > 38 indicates identity
Score > 34 indicates homology		 U K.TATLVLPVGENR.K
3022   624.3627 1870.0663 1870.0312 18.7 0 3 1.1 +2Score > 33 indicates identity
Score > 16 indicates homology		 K.GATGRPVPLLTQYLLDR.A + Deamidated (NQ)
3044 +1 943.0051 1883.9956 1883.9588 19.5 0 85 2.2e-007 +1Score > 38 indicates identity
Score > 31 indicates homology		 U K.GLNGADAVLALDDIAVSNR.D + Deamidated (NQ)
3239   675.0642 2022.1708 2022.1473 11.6 0 4 0.84 +1Score > 30 indicates identity
Score > 16 indicates homology		 U R.GDLAGKPGQTLLLQNLSGLK.A

3 subsets and intersections (3 subset proteins in total)

Score Mass Subset of
AMPA_PSEP1 44 52796 35.1
Probable cytosol aminopeptidase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=pepA PE=3 SV=1
AMPA_PSEPU 40 52835 35.1
Cytosol aminopeptidase OS=Pseudomonas putida GN=pepA PE=3 SV=1
AMPA_PSEE4 24 52459 35.1
Probable cytosol aminopeptidase OS=Pseudomonas entomophila (strain L48) GN=pepA PE=3 SV=1

+36

Accession Score Description
1 PNP_PSEPG 128 Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain GB-1) GN=pnp PE=3 SV=1

+37

Accession Score Description
1 HTPG_PSEFS 127 Chaperone protein htpG OS=Pseudomonas fluorescens (strain SBW25) GN=htpG PE=3 SV=1

+38

Accession Score Description
1 IF2_PSEE4 126 Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1

+39

Accession Score Description
1 ACP_PSEPK 126 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1

+40

Accession Score Description
1 ACON2_PSEAE 125 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1
Page: Previous 1 2 3 4 5 6 7 8 9 … 19 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/