MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita6 sp |
| MS data file | : | PRT1270_T-BRSC_6_20250714122635.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:38:56 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,869 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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31| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_PSEPK | 141 | Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1 |
32| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LEU1_PSEP1 | 139 | 2-isopropylmalate synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=leuA PE=3 SV=1 |
33| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODP2_PSEAE | 132 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 33.1 | ODP2_PSEAE |
132 | 56788 | 10 (6) | 6 (4) | 0.17 | |
| Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2 | |||||||
10 peptide matches (6 non-duplicate, 4 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 189 |
2 |
473.3105 | 472.3032 | 472.3009 | 4.85 | 0 | 25 | 0.58 | 1Score > 45 indicates identity Score > 36 indicates homology |
K.AGVVK.S | |
| 332 |
309.1856 | 616.3566 | 616.3544 | 3.65 | 1 | 17 | 0.93 | 1Score > 44 indicates identity Score > 30 indicates homology |
M.SELIR.V | ||
| 772 |
421.7471 | 841.4796 | 841.4770 | 3.18 | 0 | 55 | 0.0013 | 1Score > 39 indicates identity |
U | R.VINGAAAAR.F | |
| 1176 |
2 |
505.3605 | 1008.7064 | 1008.6947 | 11.7 | 0 | 43 | 6e-005 | 1Score > 13 indicates identity |
U | K.LTVLPILLK.A |
| 1623 |
403.8904 | 1208.6494 | 1208.6448 | 3.79 | 0 | 43 | 0.0062 | 1Score > 39 indicates identity Score > 33 indicates homology |
U | R.LMQVGAANLHR.S | |
| 2195 |
731.3681 | 1460.7216 | 1460.7147 | 4.75 | 1 | 44 | 0.00082 | 1Score > 41 indicates identity Score > 25 indicates homology |
U | K.ELPDFNSSLAPSGK.A |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| ODP2_AZOVI |
101 | 65119 | 33.1 | ||
| Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Azotobacter vinelandii PE=1 SV=3 | |||||
34| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RISB_PSEPG | 129 | 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1 |
35| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMPA_PSEPK | 129 | Probable cytosol aminopeptidase OS=Pseudomonas putida (strain KT2440) GN=pepA PE=3 SV=1 |
36| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PNP_PSEPG | 128 | Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain GB-1) GN=pnp PE=3 SV=1 |
37| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HTPG_PSEFS | 127 | Chaperone protein htpG OS=Pseudomonas fluorescens (strain SBW25) GN=htpG PE=3 SV=1 |
38| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IF2_PSEE4 | 126 | Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1 |
39| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACP_PSEPK | 126 | Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1 |
40| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACON2_PSEAE | 125 | Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1 |
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