MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 187)

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-31

Accession Score Description
1 DNAK_PSEPK 141 Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1
Score Mass Matches Sequences emPAI
31.1 DNAK_PSEPK 141 68873 6 (3) 4 (3) 0.10
Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1
1 sameset of DNAK_PSEPK
DNAK_PSEP1 141 65566 6 (3) 4 (3) 0.11
Chaperone protein dnaK OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=dnaK PE=2 SV=1

-6 peptide matches (5 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1624   606.3328 1210.6510 1210.6346 13.6 0 29 0.18 +1Score > 39 indicates identity
Score > 34 indicates homology		 K.FDLADIPPAPR.G
1829   643.3630 1284.7114 1284.7038 5.97 0 75 1.4e-005 +1Score > 39 indicates identity U K.IDDVILVGGQTR.M
3414 +1 1067.5725 2133.1304 2133.0954 16.5 1 31 0.0014 +1Score > 37 indicates identity
Score > 15 indicates homology		 U R.TTPSIVAYANDGEILVGQSAK.R
3416   712.0521 2133.1345 2133.0954 18.3 1 4 1 +1Score > 37 indicates identity
Score > 16 indicates homology		 U R.TTPSIVAYANDGEILVGQSAK.R
3937   835.0869 2502.2389 2502.1987 16.1 1 96 8.6e-008 +1Score > 38 indicates identity U K.SQVFSTADDNQSAVTIHVLQGER.K + Deamidated (NQ)

4 subsets and intersections (27 subset proteins in total)

Score Mass Subset of
DNAK_PSEMY 133 68817 31.1
Chaperone protein dnaK OS=Pseudomonas mendocina (strain ymp) GN=dnaK PE=2 SV=1
1 sameset of DNAK_PSEMY
DNAK_PSEU5 133 68693
Chaperone protein dnaK OS=Pseudomonas stutzeri (strain A1501) GN=dnaK PE=2 SV=1
DNAK_TOLAT 96 70189 31.1
Chaperone protein dnaK OS=Tolumonas auensis (strain DSM 9187 / TA4) GN=dnaK PE=2 SV=1
DNAK_PSEPG 79 68670 31.1
Chaperone protein dnaK OS=Pseudomonas putida (strain GB-1) GN=dnaK PE=2 SV=1
1 sameset of DNAK_PSEPG
DNAK_PSEPW 79 68873
Chaperone protein dnaK OS=Pseudomonas putida (strain W619) GN=dnaK PE=2 SV=1
DNAK_ACTSZ 75 68436 31.1
Chaperone protein dnaK OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=dnaK PE=2 SV=1
+21 samesets of DNAK_ACTSZ

+32

Accession Score Description
1 LEU1_PSEP1 139 2-isopropylmalate synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=leuA PE=3 SV=1

+33

Accession Score Description
1 ODP2_PSEAE 132 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2

+34

Accession Score Description
1 RISB_PSEPG 129 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1

+35

Accession Score Description
1 AMPA_PSEPK 129 Probable cytosol aminopeptidase OS=Pseudomonas putida (strain KT2440) GN=pepA PE=3 SV=1

+36

Accession Score Description
1 PNP_PSEPG 128 Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain GB-1) GN=pnp PE=3 SV=1

+37

Accession Score Description
1 HTPG_PSEFS 127 Chaperone protein htpG OS=Pseudomonas fluorescens (strain SBW25) GN=htpG PE=3 SV=1

+38

Accession Score Description
1 IF2_PSEE4 126 Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1

+39

Accession Score Description
1 ACP_PSEPK 126 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1

+40

Accession Score Description
1 ACON2_PSEAE 125 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1
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