MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 187)


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+21

Accession Score Description
1 ODO2_PSEAE 192 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+22

Accession Score Description
1 RS2_PSEP1 175 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

+23

Accession Score Description
1 ODB2_PSEPU 167 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+24

Accession Score Description
1 RL5_PSEE4 164 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1

+25

Accession Score Description
1 GUAA_CELJU 162 GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1

+26

Accession Score Description
1 RL3_PSEE4 161 50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1

+27

Accession Score Description
1 RS16_PSEP1 149 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1

+28

Accession Score Description
1 ATPF_PSEPK 149 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

+29

Accession Score Description
1 SAHH_PSEP1 143 Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1

-30

Accession Score Description
1 RL2_PSEP1 142 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1
Score Mass Matches Sequences emPAI
30.1 RL2_PSEP1 142 29822 10 (5) 8 (4) 0.35
50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1
4 samesets of RL2_PSEP1
RL2_PSEPK 142 29822 10 (5) 8 (4) 0.35
50S ribosomal protein L2 OS=Pseudomonas putida (strain KT2440) GN=rplB PE=3 SV=1
RL2_PSEPW 142 29822 10 (5) 8 (4) 0.35
50S ribosomal protein L2 OS=Pseudomonas putida (strain W619) GN=rplB PE=3 SV=1
RL2_PSEE4 142 29808 9 (5) 7 (4) 0.35
50S ribosomal protein L2 OS=Pseudomonas entomophila (strain L48) GN=rplB PE=3 SV=1
RL2_PSEPG 142 29794 8 (5) 6 (4) 0.35
50S ribosomal protein L2 OS=Pseudomonas putida (strain GB-1) GN=rplB PE=3 SV=1

-10 peptide matches (8 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
378 +1 325.1880 648.3614 648.3595 2.99 0 30 0.045 +1Score > 40 indicates identity
Score > 29 indicates homology
U R.LVDFR.R
505   352.7217 703.4288 703.4268 2.86 0 27 0.11 +1Score > 35 indicates identity
Score > 30 indicates homology
R.YIIAPK.G
819   429.7433 857.4720 857.4719 0.18 0 36 0.18 +1Score > 43 indicates identity
Score > 41 indicates homology
U K.AGNSLQLR.N
994   468.7628 935.5110 935.5076 3.65 1 10 1.4 +5Score > 40 indicates identity
Score > 24 indicates homology
U R.EGVYVTLR.L
1263   522.7984 1043.5822 1043.5723 9.51 0 57 0.00076 +1Score > 40 indicates identity
Score > 38 indicates homology
U R.SAGASAQLIAR.E
1751   626.8309 1251.6472 1251.6401 5.74 0 33 0.048 +1Score > 40 indicates identity
Score > 33 indicates homology
U R.HPVSPWGFPTK.G
2465 +1 790.9648 1579.9150 1579.8933 13.7 0 93 5.7e-008 +1Score > 33 indicates identity U K.GVSAGDQLIAGALAPIK.A
2827   440.7597 1759.0097 1758.9992 5.96 1 7 0.65 +1Score > 32 indicates identity
Score > 17 indicates homology
U R.NIPVGSTIHGIELKPGK.G

4 subsets and intersections (131 subset proteins in total)

Score Mass Subset of
RL2_PSE14 54 29789 30.1
50S ribosomal protein L2 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplB PE=3 SV=1
5 samesets of RL2_PSE14
RL2_PSEFS 54 29803
50S ribosomal protein L2 OS=Pseudomonas fluorescens (strain SBW25) GN=rplB PE=3 SV=1
RL2_PSEPF 54 29773
50S ribosomal protein L2 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplB PE=3 SV=1
RL2_PSESM 54 29833
50S ribosomal protein L2 OS=Pseudomonas syringae pv. tomato GN=rplB PE=3 SV=1
RL2_PSEU2 54 29789
50S ribosomal protein L2 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplB PE=3 SV=1
RL2_PSEMY 54 29847
50S ribosomal protein L2 OS=Pseudomonas mendocina (strain ymp) GN=rplB PE=3 SV=1
RL2_NEOSM 33 30311 30.1
50S ribosomal protein L2 OS=Neorickettsia sennetsu (strain Miyayama) GN=rplB PE=3 SV=1
RL2_AZOVD 33 29689 30.1
50S ribosomal protein L2 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplB PE=3 SV=1
2 samesets of RL2_AZOVD
RL2_PSEF5 33 29745
50S ribosomal protein L2 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplB PE=3 SV=1
RL2_PSEU5 33 29761
50S ribosomal protein L2 OS=Pseudomonas stutzeri (strain A1501) GN=rplB PE=3 SV=1
ACSL1_HUMAN 30 0 30.1
description
+120 samesets of ACSL1_HUMAN

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