MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 187)

Page: 1 2 3 4 5 6 … 19 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1352 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
4 EFTU_CYAP7 34 Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1
3 EFTU_CARRP 180 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1236 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 1121 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSEFS 949 ATP synthase subunit beta OS=Pseudomonas fluorescens (strain SBW25) GN=atpD PE=3 SV=1

-3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 954 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 173 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 163 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
ATPA_PSEPK 954 55489 62 (38) 28 (18) 1.43
ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
1 sameset of ATPA_PSEPK
ATPA_PSEP1 954 55458 62 (38) 28 (18) 1.43
ATP synthase subunit alpha OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpA PE=3 SV=1
ATPA_RICAH 173 56389 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
7 samesets of ATPA_RICAH
ATPA_RICCN 173 56128 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia conorii GN=atpA PE=3 SV=2
ATPA_RICFE 173 56172 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia felis GN=atpA PE=3 SV=1
ATPA_RICPU 173 56160 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia peacockii (strain Rustic) GN=atpA PE=3 SV=1
ATPA_RICRO 173 56195 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia rickettsii (strain Iowa) GN=atpA PE=3 SV=2
ATPA_RICRS 173 56195 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia rickettsii (strain Sheila Smith) GN=atpA PE=3 SV=1
ATPA_RICM5 173 56127 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia massiliae (strain Mtu5) GN=atpA PE=3 SV=2
ATPA_RICB8 173 56616 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia bellii (strain OSU 85-389) GN=atpA PE=3 SV=1
ATPA_VEREI 163 57757 12 (5) 6 (2) 0.17
ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

-69 peptide matches (41 non-duplicate, 28 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
228   517.3118 516.3045 516.3020 4.94 1 5 1.4 +5Score > 43 indicates identity
Score > 19 indicates homology		 X E.KNGAK.W
296 +1 301.6855 601.3564 601.3548 2.82 0 25 0.86 +1Score > 43 indicates identity
Score > 37 indicates homology		 X K.LSGGIR.T
297   602.3638 601.3565 601.3548 2.95 0 18 0.78 +2Score > 43 indicates identity
Score > 29 indicates homology		 X K.LSGGIR.T
305   606.2903 605.2830 605.2809 3.46 0 14 0.12 +1Score > 40 indicates identity
Score > 17 indicates homology		 U X K.ATQTW.-
361 +1 318.1804 634.3462 634.3438 3.79 0 31 0.043 +1Score > 41 indicates identity
Score > 30 indicates homology		 U X E.LAQFR.E + Deamidated (NQ)
508   354.1961 706.3776 706.3762 2.04 0 23 0.44 +1Score > 41 indicates identity
Score > 32 indicates homology		 X X K.QAVAYR.Q
509   707.3862 706.3789 706.3762 3.85 0 35 0.21 +1Score > 41 indicates identity X X K.QAVAYR.Q
529   360.7030 719.3914 719.3887 3.75 1 27 0.069 +1Score > 41 indicates identity
Score > 28 indicates homology		 X R.VTELMK.Q
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_6_20250714122635.raw

Score > 36 indicates identity

Score > 34 indicates homology
5.70 1 22 0.85 1 KISINR  
5.68 1 22 0.85 1 KLSIGGR  
5.70 1 20 1.3 3 KISNIR  
5.70 1 20 1.3 3 KISNLR  
554   365.7342 729.4538 729.4497 5.68 1 20 1.3 -3Score > 36 indicates identity
Score > 34 indicates homology		 X K.KLSGGIR.T
5.70 1 20 1.3 3 KLSNIR  
5.70 1 20 1.3 3 KLSNLR  
5.68 1 18 2.1 8 KTVLNR  
5.68 1 15 4.4 9 KTVNIR  
5.68 1 13 7.7 10 QLSRVK  
727   408.2358 814.4570 814.4548 2.70 1 32 0.78 +4Score > 43 indicates identity X X X R.ELIIGDR.Q
736   822.4491 821.4418 821.4395 2.79 0 34 0.17 +1Score > 40 indicates identity
Score > 38 indicates homology		 X R.TALAQYR.E
737 +1 411.7287 821.4428 821.4395 4.04 0 30 0.5 +1Score > 40 indicates identity
Score > 39 indicates homology		 X R.TALAQYR.E
775   842.5519 841.5446 841.5385 7.26 0 38 0.03 +1Score > 35 indicates identity U X X R.QISLLLR.R
779 +3 421.7802 841.5458 841.5385 8.72 0 42 0.011 +1Score > 35 indicates identity U X X R.QISLLLR.R
810 +2 427.7725 853.5304 853.5273 3.69 1 43 0.0059 +1Score > 33 indicates identity U X R.ILEVPVGK.E
825   859.5033 858.4960 858.4923 4.33 0 45 0.025 +1Score > 42 indicates identity U X R.STVANIVR.K
826 +1 430.2554 858.4962 858.4923 4.59 0 35 0.25 +1Score > 42 indicates identity
Score > 42 indicates homology		 U X R.STVANIVR.K
903   447.7416 893.4686 893.4607 8.90 0 38 0.018 +1Score > 41 indicates identity
Score > 33 indicates homology		 U X K.FTNGAVTGK.T
904   448.2316 894.4486 894.4447 4.41 0 53 0.0012 +1Score > 41 indicates identity
Score > 37 indicates homology		 U X K.FTNGAVTGK.T + Deamidated (NQ)
906 +2 449.2711 896.5276 896.5232 4.93 0 41 0.012 +1Score > 36 indicates identity
Score > 35 indicates homology		 U X K.VAPGVIWR.K
1203   339.5303 1015.5691 1015.5774 -8.22 1 14 6.1 +10Score > 40 indicates identity
Score > 34 indicates homology		 U X K.RSTVANIVR.K + Deamidated (NQ)
1260   522.7813 1043.5480 1043.5434 4.49 0 19 0.38 +1Score > 42 indicates identity
Score > 27 indicates homology		 U X K.SVDAMIPVGR.G
1371 +3 553.8150 1105.6154 1105.6019 12.2 1 43 0.002 +1Score > 38 indicates identity
Score > 29 indicates homology		 U X R.GFLIDVEVSK.I
1597 +2 599.3299 1196.6452 1196.6401 4.30 0 65 0.00015 +1Score > 39 indicates identity U X X R.VVDALGNPIDGK.G
1648 +1 609.3120 1216.6094 1216.6048 3.85 0 69 9.3e-005 +1Score > 42 indicates identity U X R.IDNLDVSSQAR.N
1662 +1 611.3113 1220.6080 1220.6037 3.54 0 43 0.00065 +1Score > 42 indicates identity
Score > 24 indicates homology		 U X K.SVDQPVQTGYK.S
1833 +1 644.8574 1287.7002 1287.6856 11.3 0 37 0.041 +1Score > 40 indicates identity
Score > 36 indicates homology		 U X K.TAMAIDAIINQK.D
1843 +2 647.3045 1292.5944 1292.5885 4.64 1 102 3e-008 +1Score > 40 indicates identity
Score > 39 indicates homology		 U X K.GDFNDEIDAGLK.A
1928 +1 665.3384 1328.6622 1328.6572 3.79 1 94 3.7e-008 +1Score > 41 indicates identity
Score > 32 indicates homology		 U X K.GPLGNTQTDAVEK.V
1964   675.3607 1348.7068 1348.6987 6.05 1 74 4.1e-006 +1Score > 40 indicates identity
Score > 33 indicates homology		 U X R.KSVDQPVQTGYK.S
2147   477.5847 1429.7323 1429.7273 3.45 1 23 0.11 +1Score > 41 indicates identity
Score > 26 indicates homology		 U X K.GRIDNLDVSSQAR.N
2148   715.8744 1429.7342 1429.7273 4.83 1 61 0.00012 +1Score > 41 indicates identity
Score > 34 indicates homology		 U X K.GRIDNLDVSSQAR.N
2151 +2 716.3801 1430.7456 1430.7365 6.36 1 78 3.7e-006 +1Score > 41 indicates identity
Score > 37 indicates homology		 U X R.NEGTVVSVSDGIVR.I
2392   777.3772 1552.7398 1552.7310 5.68 1 86 1.8e-006 +1Score > 41 indicates identity U X X X R.EAYPGDVFYLHSR.L
2394 +1 518.5883 1552.7431 1552.7310 7.75 1 71 5.1e-005 +1Score > 41 indicates identity
Score > 41 indicates homology		 U X X X R.EAYPGDVFYLHSR.L
2535 +1 806.9388 1611.8630 1611.8409 13.7 1 90 2.8e-007 +1Score > 39 indicates identity
Score > 37 indicates homology		 U X K.IGSFEQALIAFFNR.D
2536   538.2952 1611.8638 1611.8409 14.2 1 79 5.5e-006 +1Score > 39 indicates identity U X K.IGSFEQALIAFFNR.D
2734   854.9288 1707.8430 1707.8315 6.75 1 65 0.00012 +1Score > 41 indicates identity
Score > 39 indicates homology		 U X R.DRGQDALIVYDDLSK.Q + Deamidated (NQ)
2735 +2 570.2885 1707.8437 1707.8315 7.11 1 46 0.001 +1Score > 41 indicates identity
Score > 29 indicates homology		 U X R.DRGQDALIVYDDLSK.Q + Deamidated (NQ)
3350   701.3490 2101.0252 2100.9859 18.7 1 88 5.5e-007 +1Score > 39 indicates identity
Score > 38 indicates homology		 U X K.QYAPMSIADMALSLYAAER.G + Deamidated (NQ)

+43 subsets and intersections (861 subset proteins in total)

+4

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 667 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
4 CH602_SORC5 69 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
3 CH60_COLMA 82 60 kDa chaperonin OS=Colwellia maris GN=groL PE=3 SV=2
2 CH601_ECOK1 119 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1

+5

Accession Score Description
Family member distances as a dendrogram 1 RPOC_PSEPK 451 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1
4 RPOC_PELUB 108 DNA-directed RNA polymerase subunit beta' OS=Pelagibacter ubique GN=rpoC PE=3 SV=1
3 RPOC_SYNAS 149 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1
2 RPOB_PSEP1 316 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoB PE=3 SV=1

+6

Accession Score Description
1 RL1_PSEE4 451 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+7

Accession Score Description
1 OTCC_PSEPK 445 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+8

Accession Score Description
1 ARCA_PSEPK 405 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+9

Accession Score Description
1 DLDH2_PSEPU 387 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+10

Accession Score Description
1 DBHB_PSEAE 318 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3
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