MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 187)

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+21

Accession Score Description
1 ODO2_PSEAE 192 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+22

Accession Score Description
1 RS2_PSEP1 175 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

+23

Accession Score Description
1 ODB2_PSEPU 167 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+24

Accession Score Description
1 RL5_PSEE4 164 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1

+25

Accession Score Description
1 GUAA_CELJU 162 GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1

+26

Accession Score Description
1 RL3_PSEE4 161 50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1

+27

Accession Score Description
1 RS16_PSEP1 149 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1

-28

Accession Score Description
1 ATPF_PSEPK 149 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1
Score Mass Matches Sequences emPAI
28.1 ATPF_PSEPK 149 16945 12 (8) 5 (4) 0.68
ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1
1 sameset of ATPF_PSEPK
ATPF_PSEE4 149 17016 12 (8) 5 (4) 0.68
ATP synthase subunit b OS=Pseudomonas entomophila (strain L48) GN=atpF PE=3 SV=1

-12 peptide matches (6 non-duplicate, 6 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
224 +1 516.3046 515.2973 515.2955 3.57 1 19 1.4 +2Score > 47 indicates identity
Score > 33 indicates homology		 K.LAAEI.-
1094 +1 486.7604 971.5062 971.5036 2.74 1 48 0.0055 +1Score > 41 indicates identity
Score > 38 indicates homology		 U R.AAQLVDEAR.E
1192 +1 508.2639 1014.5132 1014.5094 3.79 0 41 0.037 +1Score > 40 indicates identity
Score > 39 indicates homology		 U K.IADGLDAANR.A
1532 +2 585.8299 1169.6452 1169.6404 4.12 1 66 0.00012 +1Score > 40 indicates identity U R.AQVGALAVGGAEK.I
2923 +1 603.3330 1806.9772 1806.9475 16.4 1 53 0.00012 +1Score > 37 indicates identity
Score > 26 indicates homology		 U K.ILGATIDQNAHAELVNK.L + Deamidated (NQ)
2924   603.3336 1806.9790 1806.9475 17.4 1 55 0.00078 +1Score > 37 indicates identity
Score > 36 indicates homology		 U K.ILGATIDQNAHAELVNK.L + Deamidated (NQ)

3 subsets and intersections (10 subset proteins in total)

Score Mass Subset of
ATPF_PSEP1 136 16959 28.1
ATP synthase subunit b OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpF PE=3 SV=1
2 samesets of ATPF_PSEP1
ATPF_PSEPG 136 16945
ATP synthase subunit b OS=Pseudomonas putida (strain GB-1) GN=atpF PE=3 SV=1
ATPF_PSEPW 136 16945
ATP synthase subunit b OS=Pseudomonas putida (strain W619) GN=atpF PE=3 SV=1
ATPF_PSE14 89 16988 28.1
ATP synthase subunit b OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpF PE=3 SV=1
3 samesets of ATPF_PSE14
ATPF_PSESM 89 17018
ATP synthase subunit b OS=Pseudomonas syringae pv. tomato GN=atpF PE=3 SV=1
ATPF_PSEU2 89 16988
ATP synthase subunit b OS=Pseudomonas syringae pv. syringae (strain B728a) GN=atpF PE=3 SV=1
ATPF_PSEF5 89 16981
ATP synthase subunit b OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=atpF PE=3 SV=1
ATPF_PSEA7 41 17003 28.1
ATP synthase subunit b OS=Pseudomonas aeruginosa (strain PA7) GN=atpF PE=3 SV=1
2 samesets of ATPF_PSEA7
ATPF_PSEAB 41 17003
ATP synthase subunit b OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=atpF PE=3 SV=1
ATPF_PSEAE 41 17003
ATP synthase subunit b OS=Pseudomonas aeruginosa GN=atpF PE=3 SV=1

+29

Accession Score Description
1 SAHH_PSEP1 143 Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1

+30

Accession Score Description
1 RL2_PSEP1 142 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1
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