MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 187)


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+21

Accession Score Description
1 ODO2_PSEAE 192 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+22

Accession Score Description
1 RS2_PSEP1 175 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

+23

Accession Score Description
1 ODB2_PSEPU 167 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+24

Accession Score Description
1 RL5_PSEE4 164 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1

+25

Accession Score Description
1 GUAA_CELJU 162 GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1

+26

Accession Score Description
1 RL3_PSEE4 161 50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1

-27

Accession Score Description
1 RS16_PSEP1 149 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1
Score Mass Matches Sequences emPAI
27.1 RS16_PSEP1 149 9183 6 (5) 3 (3) 1.03
30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1
2 samesets of RS16_PSEP1
RS16_PSEPG 149 9183 6 (5) 3 (3) 1.03
30S ribosomal protein S16 OS=Pseudomonas putida (strain GB-1) GN=rpsP PE=3 SV=1
RS16_PSEPK 149 9183 6 (5) 3 (3) 1.03
30S ribosomal protein S16 OS=Pseudomonas putida (strain KT2440) GN=rpsP PE=3 SV=1

+6 peptide matches (4 non-duplicate, 2 duplicate)

3 subsets and intersections (6 subset proteins in total)

Score Mass Subset of
RS16_PSEPW 109 9155 27.1
30S ribosomal protein S16 OS=Pseudomonas putida (strain W619) GN=rpsP PE=3 SV=1
RS16_PSEE4 83 9199 27.1
30S ribosomal protein S16 OS=Pseudomonas entomophila (strain L48) GN=rpsP PE=3 SV=1
RS16_PSEF5 47 9230 27.1
30S ribosomal protein S16 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsP PE=3 SV=1
3 samesets of RS16_PSEF5
RS16_PSEFS 47 9202
30S ribosomal protein S16 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsP PE=3 SV=1
RS16_PSEPF 47 9230
30S ribosomal protein S16 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsP PE=3 SV=1
RS16_PSEU5 47 9195
30S ribosomal protein S16 OS=Pseudomonas stutzeri (strain A1501) GN=rpsP PE=3 SV=1

+28

Accession Score Description
1 ATPF_PSEPK 149 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

+29

Accession Score Description
1 SAHH_PSEP1 143 Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1

+30

Accession Score Description
1 RL2_PSEP1 142 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1
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