Rita6 sp (Mascot Search Results)

Search metadata
User	:	Jennifer
E-mail	:	[email protected]
Search title	:	Rita6 sp
MS data file	:	PRT1270_T-BRSC_6_20250714122635.mgf
Database	:	SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp	:	12 Aug 2025 at 23:38:56 GMT

Search metadata

User

Jennifer

E-mail

[email protected]

Search title

Rita6 sp

MS data file

PRT1270_T-BRSC_6_20250714122635.mgf

Database

SwissProt 57.15 (515,203 sequences; 181,334,896 residues)

Timestamp

12 Aug 2025 at 23:38:56 GMT

Search parameters
Type of search	:	MS/MS Ion Search
Enzyme	:	GluC_Trypsin
Fixed modifications	:	Carbamidomethyl (C)
Variable modifications	:	Deamidated (NQ), Oxidation (M)
Mass values	:	Monoisotopic
Protein mass	:	Unrestricted
Peptide mass tolerance	:	± 20 ppm
Fragment mass tolerance	:	± 0.1 Da
Max missed cleavages	:	1
Instrument type	:	ESI-FTICR
Number of queries	:	4,869

Search parameters

Type of search

MS/MS Ion Search

Enzyme

GluC_Trypsin

Fixed modifications

Carbamidomethyl (C)

Variable modifications

Deamidated (NQ),

Oxidation (M)

Mass values

Monoisotopic

Protein mass

Unrestricted

Peptide mass tolerance

± 20 ppm

Fragment mass tolerance

± 0.1 Da

Max missed cleavages

Instrument type

ESI-FTICR

Number of queries

4,869

Legend
Dupes	Expect	Rank	U	Peptide
	0.037	2		GAYSLSLR	significant
	9	1		GFFLFVEGGR	top ranking
	6.4e-005	1		GSSIFGLAPGK	significant and top ranking
	1.3e-006	1		SSGTSYPDVLK	peptide is found in all proteins in family member 1
	6.2e-007	1		VCNYVSWIK	peptide is found in some but not all proteins in family member 2
	6.4e-005	1	U	GSSIFGLAPGK	unique
2	5.7e-005	1		LNTLETEEWFFK	peptide has two duplicates
	0.18	1		LNTLETEEWFFK	duplicate peptide

Legend

Dupes

Expect

Rank

Peptide

0.037

GAYSLSLR

significant

GFFLFVEGGR

top ranking

6.4e-005

GSSIFGLAPGK

significant and top ranking

1.3e-006

SSGTSYPDVLK

peptide is found in all proteins in family member 1

6.2e-007

VCNYVSWIK

peptide is found in some but not all proteins in family member 2

6.4e-005

GSSIFGLAPGK

unique

5.7e-005

LNTLETEEWFFK

peptide has two duplicates

0.18

LNTLETEEWFFK

duplicate peptide

Right-facing triangle (

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Protein Family Summary

Protein families 21–30 (out of 187)

Previous 1 2 3 4 5 6 7 8 … 19 Next

21

		Accession	Score	Description
	1	ODO2_PSEAE	192	Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

22

		Accession	Score	Description
	1	RS2_PSEP1	175	30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

23

		Accession	Score	Description
	1	ODB2_PSEPU	167	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

24

		Accession	Score	Description
	1	RL5_PSEE4	164	50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1

25

		Accession	Score	Description
	1	GUAA_CELJU	162	GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1

26

		Accession	Score	Description
	1	RL3_PSEE4	161	50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1

		Score	Mass	Matches	Sequences	emPAI
26.1	RL3_PSEE4	161	22600	7 (4)	4 (2)	0.48
	50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1
	4 samesets of RL3_PSEE4
	RL3_PSEP1	161	22703	7 (4)	4 (2)	0.48
	50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1
	RL3_PSEPG	161	22703	7 (4)	4 (2)	0.48
	50S ribosomal protein L3 OS=Pseudomonas putida (strain GB-1) GN=rplC PE=3 SV=1
	RL3_PSEPK	161	22703	7 (4)	4 (2)	0.48
	50S ribosomal protein L3 OS=Pseudomonas putida (strain KT2440) GN=rplC PE=3 SV=2
	RL3_PSEPW	161	22663	7 (4)	4 (2)	0.48
	50S ribosomal protein L3 OS=Pseudomonas putida (strain W619) GN=rplC PE=3 SV=1

7 peptide matches (6 non-duplicate, 1 duplicate)

Query	Dupes	Observed	Mr(expt)	Mr(calc)	ppm	M	Score	Expect	Rank	U	Peptide
1061	1	479.7727	957.5308	957.5244	6.78	1	26	0.64	1Score > 42 indicates identity Score > 36 indicates homology		R.AVQVTVGER.R
1360		367.5340	1099.5802	1099.5774	2.49	0	28	0.31	1Score > 41 indicates identity Score > 35 indicates homology		R.VTAAQAGHFAK.A
1422		565.3356	1128.6566	1128.6503	5.64	1	63	0.00017	1Score > 38 indicates identity	U	R.VTVQSLEVVR.V
2835		588.6500	1762.9282	1762.9101	10.2	1	39	0.0022	1Score > 39 indicates identity Score > 24 indicates homology	U	K.AELFTAGQLVDVTGQSK.G
2836		882.4717	1762.9288	1762.9101	10.6	1	93	1.9e-008	1Score > 39 indicates identity Score > 28 indicates homology	U	K.AELFTAGQLVDVTGQSK.G
2839		882.9719	1763.9292	1763.8942	19.9	1	53	9.8e-005	1Score > 39 indicates identity Score > 25 indicates homology	U	K.AELFTAGQLVDVTGQSK.G + Deamidated (NQ)

1 subset or intersection (18 subset proteins in total)

	Score	Mass	Subset of
RL3_ACTP2	63	22369	26.1
50S ribosomal protein L3 OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=rplC PE=3 SV=1
17 samesets of RL3_ACTP2
RL3_PSEF5	63	22646
50S ribosomal protein L3 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplC PE=3 SV=1
RL3_PSEFS	63	22664
50S ribosomal protein L3 OS=Pseudomonas fluorescens (strain SBW25) GN=rplC PE=3 SV=1
RL3_PSEPF	63	22603
50S ribosomal protein L3 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplC PE=3 SV=1
RL3_PSESM	63	22707
50S ribosomal protein L3 OS=Pseudomonas syringae pv. tomato GN=rplC PE=3 SV=1
RL3_PSEU2	63	22693
50S ribosomal protein L3 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplC PE=3 SV=1
RL3_PSEMY	63	22536
50S ribosomal protein L3 OS=Pseudomonas mendocina (strain ymp) GN=rplC PE=3 SV=1
RL3_ACTP7	63	22369
50S ribosomal protein L3 OS=Actinobacillus pleuropneumoniae serotype 7 (strain AP76) GN=rplC PE=3 SV=1
RL3_ACTPJ	63	22369
50S ribosomal protein L3 OS=Actinobacillus pleuropneumoniae serotype 3 (strain JL03) GN=rplC PE=3 SV=1
RL3_ACTSZ	63	22312
50S ribosomal protein L3 OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=rplC PE=3 SV=1
RL3_HAEI8	63	22350
50S ribosomal protein L3 OS=Haemophilus influenzae (strain 86-028NP) GN=rplC PE=3 SV=1
RL3_HAEIG	63	22349
50S ribosomal protein L3 OS=Haemophilus influenzae (strain PittGG) GN=rplC PE=3 SV=1
RL3_HAEIN	63	22350
50S ribosomal protein L3 OS=Haemophilus influenzae GN=rplC PE=3 SV=1
RL3_HAEPS	63	22385
50S ribosomal protein L3 OS=Haemophilus parasuis serovar 5 (strain SH0165) GN=rplC PE=3 SV=1
RL3_MANSM	63	22326
50S ribosomal protein L3 OS=Mannheimia succiniciproducens (strain MBEL55E) GN=rplC PE=3 SV=1
RL3_PASMU	63	22369
50S ribosomal protein L3 OS=Pasteurella multocida GN=rplC PE=3 SV=1
RL3_AGGAC	63	12929
50S ribosomal protein L3 (Fragment) OS=Aggregatibacter actinomycetemcomitans GN=rplC PE=3 SV=1
RL3_HAEDU	63	22295
50S ribosomal protein L3 OS=Haemophilus ducreyi GN=rplC PE=3 SV=1

27

		Accession	Score	Description
	1	RS16_PSEP1	149	30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1

28

		Accession	Score	Description
	1	ATPF_PSEPK	149	ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

29

		Accession	Score	Description
	1	SAHH_PSEP1	143	Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1

30

		Accession	Score	Description
	1	RL2_PSEP1	142	50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1

Previous 1 2 3 4 5 6 7 8 … 19 Next

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MASCOT Search Results

Score distribution

Protein Family Summary

Protein families 21–30 (out of 187)

21

22

23

24

25

26

7 peptide matches (6 non-duplicate, 1 duplicate)

1 subset or intersection (18 subset proteins in total)

27

28

29

30