Rita6 sp (Mascot Search Results)

Search metadata
User	:	Jennifer
E-mail	:	[email protected]
Search title	:	Rita6 sp
MS data file	:	PRT1270_T-BRSC_6_20250714122635.mgf
Database	:	SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp	:	12 Aug 2025 at 23:38:56 GMT

Search metadata

User

Jennifer

E-mail

[email protected]

Search title

Rita6 sp

MS data file

PRT1270_T-BRSC_6_20250714122635.mgf

Database

SwissProt 57.15 (515,203 sequences; 181,334,896 residues)

Timestamp

12 Aug 2025 at 23:38:56 GMT

Search parameters
Type of search	:	MS/MS Ion Search
Enzyme	:	GluC_Trypsin
Fixed modifications	:	Carbamidomethyl (C)
Variable modifications	:	Deamidated (NQ), Oxidation (M)
Mass values	:	Monoisotopic
Protein mass	:	Unrestricted
Peptide mass tolerance	:	± 20 ppm
Fragment mass tolerance	:	± 0.1 Da
Max missed cleavages	:	1
Instrument type	:	ESI-FTICR
Number of queries	:	4,869

Search parameters

Type of search

MS/MS Ion Search

Enzyme

GluC_Trypsin

Fixed modifications

Carbamidomethyl (C)

Variable modifications

Deamidated (NQ),

Oxidation (M)

Mass values

Monoisotopic

Protein mass

Unrestricted

Peptide mass tolerance

± 20 ppm

Fragment mass tolerance

± 0.1 Da

Max missed cleavages

Instrument type

ESI-FTICR

Number of queries

4,869

Legend
Dupes	Expect	Rank	U	Peptide
	0.037	2		GAYSLSLR	significant
	9	1		GFFLFVEGGR	top ranking
	6.4e-005	1		GSSIFGLAPGK	significant and top ranking
	1.3e-006	1		SSGTSYPDVLK	peptide is found in all proteins in family member 1
	6.2e-007	1		VCNYVSWIK	peptide is found in some but not all proteins in family member 2
	6.4e-005	1	U	GSSIFGLAPGK	unique
2	5.7e-005	1		LNTLETEEWFFK	peptide has two duplicates
	0.18	1		LNTLETEEWFFK	duplicate peptide

Legend

Dupes

Expect

Rank

Peptide

0.037

GAYSLSLR

significant

GFFLFVEGGR

top ranking

6.4e-005

GSSIFGLAPGK

significant and top ranking

1.3e-006

SSGTSYPDVLK

peptide is found in all proteins in family member 1

6.2e-007

VCNYVSWIK

peptide is found in some but not all proteins in family member 2

6.4e-005

GSSIFGLAPGK

unique

5.7e-005

LNTLETEEWFFK

peptide has two duplicates

0.18

LNTLETEEWFFK

duplicate peptide

Right-facing triangle (

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Protein Family Summary

Protein families 21–30 (out of 187)

Previous 1 2 3 4 5 6 7 8 … 19 Next

21

		Accession	Score	Description
	1	ODO2_PSEAE	192	Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

22

		Accession	Score	Description
	1	RS2_PSEP1	175	30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

23

		Accession	Score	Description
	1	ODB2_PSEPU	167	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

24

		Accession	Score	Description
	1	RL5_PSEE4	164	50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1

		Score	Mass	Matches	Sequences	emPAI
24.1	RL5_PSEE4	164	20385	15 (10)	8 (5)	0.92
	50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1
	4 samesets of RL5_PSEE4
	RL5_PSEP1	164	20316	15 (10)	8 (5)	0.92
	50S ribosomal protein L5 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplE PE=3 SV=1
	RL5_PSEPG	164	20316	15 (10)	8 (5)	0.92
	50S ribosomal protein L5 OS=Pseudomonas putida (strain GB-1) GN=rplE PE=3 SV=1
	RL5_PSEPK	164	20316	15 (10)	8 (5)	0.92
	50S ribosomal protein L5 OS=Pseudomonas putida (strain KT2440) GN=rplE PE=3 SV=1
	RL5_PSEPW	164	20399	15 (10)	8 (5)	0.92
	50S ribosomal protein L5 OS=Pseudomonas putida (strain W619) GN=rplE PE=3 SV=1

15 peptide matches (9 non-duplicate, 6 duplicate)

Query	Dupes	Observed	Mr(expt)	Mr(calc)	ppm	M	Score	Expect	Rank	U	Peptide
412		671.3743	670.3670	670.3650	3.08	1	28	0.52	3Score > 37 indicates identity		R.NEIAPK.L
442		340.6806	679.3466	679.3442	3.61	1	11	3.8	6Score > 40 indicates identity Score > 29 indicates homology		K.FPFRN.-
587		375.7303	749.4460	749.4436	3.32	1	23	0.42	1Score > 36 indicates identity Score > 32 indicates homology		R.KSIAGFK.V
875	2	441.7961	881.5776	881.5698	8.89	0	56	9.9e-005	1Score > 28 indicates identity	U	R.LLAISLPR.V
879	1	443.2481	884.4816	884.4756	6.84	1	29	0.055	1Score > 39 indicates identity Score > 29 indicates homology	U	R.EGWPIGVK.V
1264		523.2789	1044.5432	1044.5274	15.2	1	22	0.049	1Score > 42 indicates identity Score > 21 indicates homology	U	K.LSNVMEVPR.V + Deamidated (NQ)
1509	2	581.3322	1160.6498	1160.6401	8.39	0	51	0.0032	1Score > 39 indicates identity	U	R.GLDITLTTTAR.S
1888	1	439.5960	1315.7662	1315.7612	3.76	0	51	6e-005	1Score > 35 indicates identity Score > 21 indicates homology	U	K.ITGQKPVVTFAR.K
1890		658.8908	1315.7670	1315.7612	4.42	0	42	0.0047	1Score > 34 indicates identity Score > 31 indicates homology	U	K.ITGQKPVVTFAR.K

3 subsets and intersections (13 subset proteins in total)

	Score	Mass	Subset of
RL5_AZOVD	52	20433	24.1
50S ribosomal protein L5 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplE PE=3 SV=1
6 samesets of RL5_AZOVD
RL5_PSEU5	52	20384
50S ribosomal protein L5 OS=Pseudomonas stutzeri (strain A1501) GN=rplE PE=3 SV=1
RL5_PSEA7	52	20393
50S ribosomal protein L5 OS=Pseudomonas aeruginosa (strain PA7) GN=rplE PE=3 SV=1
RL5_PSEA8	52	20379
50S ribosomal protein L5 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplE PE=3 SV=1
RL5_PSEAB	52	20379
50S ribosomal protein L5 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplE PE=3 SV=1
RL5_PSEAE	52	20379
50S ribosomal protein L5 OS=Pseudomonas aeruginosa GN=rplE PE=3 SV=1
RL5_PSEMY	52	20437
50S ribosomal protein L5 OS=Pseudomonas mendocina (strain ymp) GN=rplE PE=3 SV=1
RL5_PSESM	29	20407	24.1
50S ribosomal protein L5 OS=Pseudomonas syringae pv. tomato GN=rplE PE=3 SV=1
RL5_PSE14	26	20460	24.1
50S ribosomal protein L5 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplE PE=3 SV=1
4 samesets of RL5_PSE14
RL5_PSEF5	26	20474
50S ribosomal protein L5 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplE PE=3 SV=1
RL5_PSEFS	26	20490
50S ribosomal protein L5 OS=Pseudomonas fluorescens (strain SBW25) GN=rplE PE=3 SV=1
RL5_PSEPF	26	20474
50S ribosomal protein L5 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplE PE=3 SV=1
RL5_PSEU2	26	20460
50S ribosomal protein L5 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplE PE=3 SV=1

25

		Accession	Score	Description
	1	GUAA_CELJU	162	GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1

26

		Accession	Score	Description
	1	RL3_PSEE4	161	50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1

27

		Accession	Score	Description
	1	RS16_PSEP1	149	30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1

28

		Accession	Score	Description
	1	ATPF_PSEPK	149	ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

29

		Accession	Score	Description
	1	SAHH_PSEP1	143	Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1

30

		Accession	Score	Description
	1	RL2_PSEP1	142	50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1

Previous 1 2 3 4 5 6 7 8 … 19 Next

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MASCOT Search Results

Score distribution

Protein Family Summary

Protein families 21–30 (out of 187)

21

22

23

24

15 peptide matches (9 non-duplicate, 6 duplicate)

3 subsets and intersections (13 subset proteins in total)

25

26

27

28

29

30