MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 187)

Page: Previous 1 2 3 4 5 6 7 8 … 19 Next 

+21

Accession Score Description
1 ODO2_PSEAE 192 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

-22

Accession Score Description
1 RS2_PSEP1 175 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1
Score Mass Matches Sequences emPAI
22.1 RS2_PSEP1 175 27063 18 (7) 9 (4) 0.39
30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1
2 samesets of RS2_PSEP1
RS2_PSEPK 175 27063 18 (7) 9 (4) 0.39
30S ribosomal protein S2 OS=Pseudomonas putida (strain KT2440) GN=rpsB PE=3 SV=1
RS2_PSEPW 175 27095 11 (7) 7 (4) 0.39
30S ribosomal protein S2 OS=Pseudomonas putida (strain W619) GN=rpsB PE=3 SV=1

-18 peptide matches (11 non-duplicate, 7 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
539 +1 363.7017 725.3888 725.3860 3.86 0 9 0.59 +1Score > 37 indicates identity
Score > 20 indicates homology		 K.YIFGAR.N
656 +3 389.2489 776.4832 776.4796 4.66 0 30 0.057 +1Score > 33 indicates identity
Score > 31 indicates homology		 K.ILFVGTK.R
817   429.2466 856.4786 856.4766 2.35 1 38 0.1 +1Score > 40 indicates identity U K.IVAEQAAR.C
823   430.2494 858.4842 858.4810 3.73 1 60 0.0011 +1Score > 43 indicates identity U R.IAITEANK.L
1080 +1 322.5318 964.5736 964.5705 3.14 1 15 0.5 +1Score > 34 indicates identity
Score > 24 indicates homology		 U K.IHIVNLEK.T
1081   483.2944 964.5742 964.5705 3.84 1 7 0.62 +1Score > 34 indicates identity
Score > 18 indicates homology		 U K.IHIVNLEK.T
1382   370.5256 1108.5550 1108.5526 2.10 0 32 0.016 +1Score > 41 indicates identity
Score > 27 indicates homology		 U K.AGVHFGHQTR.Y
1563   591.8092 1181.6038 1181.5903 11.5 0 18 0.14 +1Score > 40 indicates identity
Score > 22 indicates homology		 R.WLGGMLTNYK.T
2603 +1 820.4214 1638.8282 1638.8076 12.6 1 57 4.6e-005 +1Score > 40 indicates identity
Score > 26 indicates homology		 U K.TLPMFNDALSFVER.L
4404   1032.8812 3095.6218 3095.5663 17.9 1 72 9.3e-006 +1Score > 34 indicates identity U K.LGIPVIGVVDTNSSPEGVDYIIPGNDDAIR.A + Deamidated (NQ)
4405 +1 1032.8815 3095.6227 3095.5663 18.2 1 57 0.00028 +1Score > 34 indicates identity U K.LGIPVIGVVDTNSSPEGVDYIIPGNDDAIR.A + Deamidated (NQ)

7 subsets and intersections (316 subset proteins in total)

Score Mass Subset of
RS2_PSE14 115 27257 22.1
30S ribosomal protein S2 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsB PE=3 SV=1
6 samesets of RS2_PSE14
RS2_PSEPG 115 27032
30S ribosomal protein S2 OS=Pseudomonas putida (strain GB-1) GN=rpsB PE=3 SV=1
RS2_PSEF5 115 27057
30S ribosomal protein S2 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsB PE=3 SV=1
RS2_PSEMY 115 27219
30S ribosomal protein S2 OS=Pseudomonas mendocina (strain ymp) GN=rpsB PE=3 SV=1
RS2_PSEFS 115 27135
30S ribosomal protein S2 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsB PE=3 SV=1
RS2_PSEPF 115 27102
30S ribosomal protein S2 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsB PE=3 SV=1
RS2_PSESM 115 27241
30S ribosomal protein S2 OS=Pseudomonas syringae pv. tomato GN=rpsB PE=3 SV=1
RS2_PSEU2 112 27302 22.1
30S ribosomal protein S2 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsB PE=3 SV=1
RS2_PSEU5 96 27306 22.1
30S ribosomal protein S2 OS=Pseudomonas stutzeri (strain A1501) GN=rpsB PE=3 SV=1
RS2_AZOVD 92 27383 22.1
30S ribosomal protein S2 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsB PE=3 SV=1
RS2_POLNS 60 27612 22.1
30S ribosomal protein S2 OS=Polynucleobacter necessarius (strain STIR1) GN=rpsB PE=3 SV=1
1 sameset of RS2_POLNS
RS2_POLSQ 60 27490
30S ribosomal protein S2 OS=Polynucleobacter sp. (strain QLW-P1DMWA-1) GN=rpsB PE=3 SV=1
RS2_PSEA7 57 27360 22.1
30S ribosomal protein S2 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsB PE=3 SV=1
4 samesets of RS2_PSEA7
RS2_PSEE4 57 27049
30S ribosomal protein S2 OS=Pseudomonas entomophila (strain L48) GN=rpsB PE=3 SV=1
RS2_PSEA8 57 27376
30S ribosomal protein S2 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsB PE=3 SV=1
RS2_PSEAB 57 27376
30S ribosomal protein S2 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsB PE=3 SV=1
RS2_PSEAE 57 27376
30S ribosomal protein S2 OS=Pseudomonas aeruginosa GN=rpsB PE=3 SV=2
RR2_CHLAT 32 25793 22.1
30S ribosomal protein S2, chloroplastic OS=Chlorokybus atmophyticus GN=rps2 PE=3 SV=2
+298 samesets of RR2_CHLAT

+23

Accession Score Description
1 ODB2_PSEPU 167 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+24

Accession Score Description
1 RL5_PSEE4 164 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1

+25

Accession Score Description
1 GUAA_CELJU 162 GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1

+26

Accession Score Description
1 RL3_PSEE4 161 50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1

+27

Accession Score Description
1 RS16_PSEP1 149 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1

+28

Accession Score Description
1 ATPF_PSEPK 149 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

+29

Accession Score Description
1 SAHH_PSEP1 143 Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1

+30

Accession Score Description
1 RL2_PSEP1 142 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1
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