MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 187)

Page: Previous 1 2 3 4 5 6 7 … 19 Next 

+11

Accession Score Description
1 RL11_PSEP1 297 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1

+12

Accession Score Description
1 SUCC_PSEPK 292 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+13

Accession Score Description
1 EFG1_PSEPK 288 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+14

Accession Score Description
1 ADHP_ECOLI 282 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+15

Accession Score Description
1 PORF_PSESY 281 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1

+16

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSEE4 276 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1
2 RL4_PSE14 181 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+17

Accession Score Description
1 RS5_PSEE4 265 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+18

Accession Score Description
1 DHSA_ECOLI 258 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

-19

Accession Score Description
1 RPOA_PSEPK 249 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1
Score Mass Matches Sequences emPAI
19.1 RPOA_PSEPK 249 36721 17 (11) 8 (6) 0.63
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1
2 samesets of RPOA_PSEPK
RPOA_PSEP1 249 36721 17 (11) 8 (6) 0.63
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoA PE=3 SV=1
RPOA_PSEE4 249 36721 17 (11) 8 (6) 0.63
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas entomophila (strain L48) GN=rpoA PE=3 SV=1

-17 peptide matches (10 non-duplicate, 7 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
347 +1 315.1855 628.3564 628.3544 3.23 0 22 0.41 +1Score > 37 indicates identity
Score > 31 indicates homology		 K.TPNLGK.K
1086 +1 485.7842 969.5538 969.5495 4.52 1 28 0.41 +1Score > 39 indicates identity
Score > 37 indicates homology		 U K.ITLEPLER.G
1236 +1 517.7869 1033.5592 1033.5556 3.51 1 42 0.006 +1Score > 40 indicates identity
Score > 33 indicates homology		 U R.IAYVVENAR.V
1457 +1 570.8097 1139.6048 1139.5975 6.46 0 39 0.0046 +1Score > 39 indicates identity
Score > 28 indicates homology		 U R.LDNWPPASLK.K
1460   381.5416 1141.6030 1141.5992 3.27 0 37 0.009 +1Score > 40 indicates identity
Score > 29 indicates homology		 U R.GFGHTLGNALR.R
1696 +1 616.8414 1231.6682 1231.6561 9.88 0 56 0.00038 +1Score > 40 indicates identity
Score > 34 indicates homology		 U R.LQLDASFSPVR.R
1748   417.5683 1249.6831 1249.6779 4.14 0 36 0.002 +1Score > 39 indicates identity
Score > 22 indicates homology		 U R.HIDVQVVSPTR.A
1749 +1 625.8494 1249.6842 1249.6779 5.08 0 62 4.6e-005 +1Score > 39 indicates identity
Score > 31 indicates homology		 U R.HIDVQVVSPTR.A
2428   523.2803 1566.8191 1566.8042 9.51 1 60 3.6e-005 +1Score > 39 indicates identity
Score > 28 indicates homology		 U K.AENIYYIGDLIQR.T
2430 +1 784.4228 1566.8310 1566.8042 17.2 1 63 2.4e-005 +1Score > 39 indicates identity
Score > 29 indicates homology		 U K.AENIYYIGDLIQR.T

7 subsets and intersections (186 subset proteins in total)

Score Mass Subset of
RPOA_PSE14 212 36765 19.1
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpoA PE=3 SV=1
5 samesets of RPOA_PSE14
RPOA_PSEPF 212 36723
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpoA PE=3 SV=1
RPOA_PSEPU 212 36695
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida GN=rpoA PE=3 SV=1
RPOA_PSESM 212 36765
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas syringae pv. tomato GN=rpoA PE=3 SV=1
RPOA_PSEU2 212 36765
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpoA PE=3 SV=1
RPOA_PSEF5 212 36721
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpoA PE=3 SV=1
RPOA_PSEU5 202 36620 19.1
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas stutzeri (strain A1501) GN=rpoA PE=3 SV=1
RPOA_PSEA7 188 36741 19.1
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas aeruginosa (strain PA7) GN=rpoA PE=3 SV=1
3 samesets of RPOA_PSEA7
RPOA_PSEAB 188 36741
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpoA PE=3 SV=1
RPOA_PSEAE 188 36741
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas aeruginosa GN=rpoA PE=3 SV=2
RPOA_PSEMY 188 36661
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas mendocina (strain ymp) GN=rpoA PE=3 SV=1
RPOA_SACD2 178 36391 19.1
DNA-directed RNA polymerase subunit alpha OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rpoA PE=3 SV=1
RPOA_AZOSB 140 35767 19.1
DNA-directed RNA polymerase subunit alpha OS=Azoarcus sp. (strain BH72) GN=rpoA PE=3 SV=1
5 samesets of RPOA_AZOSB
RPOA_CELJU 140 36326
DNA-directed RNA polymerase subunit alpha OS=Cellvibrio japonicus (strain Ueda107) GN=rpoA PE=3 SV=1
RPOA_CHRSD 140 37113
DNA-directed RNA polymerase subunit alpha OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=rpoA PE=3 SV=1
RPOA_THISH 140 36890
DNA-directed RNA polymerase subunit alpha OS=Thioalkalivibrio sp. (strain HL-EbGR7) GN=rpoA PE=3 SV=1
RPOA_AZOSE 140 35666
DNA-directed RNA polymerase subunit alpha OS=Azoarcus sp. (strain EbN1) GN=rpoA PE=3 SV=1
RPOA_LEPCP 140 36343
DNA-directed RNA polymerase subunit alpha OS=Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) GN=rpoA PE=3 SV=1
RPOA_ACIAC 131 36282 19.1
DNA-directed RNA polymerase subunit alpha OS=Acidovorax avenae subsp. citrulli (strain AAC00-1) GN=rpoA PE=3 SV=1
+52 samesets of RPOA_ACIAC
RPOA1_PSYIN 37 36427 19.1
DNA-directed RNA polymerase subunit alpha 1 OS=Psychromonas ingrahamii (strain 37) GN=rpoA1 PE=3 SV=1
+114 samesets of RPOA1_PSYIN

+20

Accession Score Description
1 RL18_PSEP1 222 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
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