MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 181–187 (out of 187)


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+181

Accession Score Description
1 LOX4_ORYSJ 22 Probable lipoxygenase 4 OS=Oryza sativa subsp. japonica GN=Os03g0700700 PE=2 SV=1

+182

Accession Score Description
1 FBPC2_RHOSR 20 Fe(3+) ions import ATP-binding protein fbpC 2 OS=Rhodococcus sp. (strain RHA1) GN=fbpC2 PE=3 SV=1

+183

Accession Score Description
1 UGPI6_ARATH 20 Uncharacterized GPI-anchored protein At1g61900 OS=Arabidopsis thaliana GN=At1g61900 PE=1 SV=1

+184

Accession Score Description
1 POLG_DEN27 20 Genome polyprotein OS=Dengue virus type 2 (strain 16681-PDK53) PE=1 SV=1

+185

Accession Score Description
1 RR3_PHAAO 19 30S ribosomal protein S3, chloroplastic OS=Phalaenopsis aphrodite subsp. formosana GN=rps3 PE=3 SV=1

-186

Accession Score Description
1 SAGE1_HUMAN 15 Sarcoma antigen 1 OS=Homo sapiens GN=SAGE1 PE=2 SV=2
Score Mass Matches Sequences emPAI
186.1 SAGE1_HUMAN 15 99618 1 (1) 1 (1) 0.02
Sarcoma antigen 1 OS=Homo sapiens GN=SAGE1 PE=2 SV=2

+1 peptide matches (1 non-duplicate, 0 duplicate)


+187

Accession Score Description
1 MBHM_ECOLI 14 Hydrogenase-2 large chain OS=Escherichia coli (strain K12) GN=hybC PE=1 SV=2
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