MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 171–180 (out of 187)


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+171

Accession Score Description
1 ARGC_AZOVD 25 N-acetyl-gamma-glutamyl-phosphate reductase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=argC PE=3 SV=1

+172

Accession Score Description
1 RL1_PSYWF 25 50S ribosomal protein L1 OS=Psychrobacter sp. (strain PRwf-1) GN=rplA PE=3 SV=1

+173

Accession Score Description
1 RS8_WOLPP 24 30S ribosomal protein S8 OS=Wolbachia pipientis subsp. Culex pipiens (strain wPip) GN=rpsH PE=3 SV=1

+174

Accession Score Description
1 ILVC_METFK 24 Ketol-acid reductoisomerase OS=Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) GN=ilvC PE=3 SV=1

+175

Accession Score Description
1 DLDH1_PSEPU 23 Dihydrolipoyl dehydrogenase OS=Pseudomonas putida GN=lpdV PE=1 SV=1

+176

Accession Score Description
1 NUSB_PSYA2 23 N utilization substance protein B homolog OS=Psychrobacter arcticus (strain DSM 17307 / 273-4) GN=nusB PE=3 SV=1

+177

Accession Score Description
1 APT_METJA 23 Adenine phosphoribosyltransferase OS=Methanocaldococcus jannaschii GN=apt PE=3 SV=1

+178

Accession Score Description
1 ALKD_PSEPU 23 2-dehydro-3-deoxy-phosphogluconate aldolase OS=Pseudomonas putida GN=eda PE=1 SV=2

+179

Accession Score Description
1 ACEA_ECOLI 23 Isocitrate lyase OS=Escherichia coli (strain K12) GN=aceA PE=1 SV=1

-180

Accession Score Description
1 RPA34_SCHPO 22 DNA-directed RNA polymerase I subunit rpa34 OS=Schizosaccharomyces pombe GN=rpa34 PE=1 SV=1
Score Mass Matches Sequences emPAI
180.1 RPA34_SCHPO 22 27993 1 (1) 1 (1) 0.08
DNA-directed RNA polymerase I subunit rpa34 OS=Schizosaccharomyces pombe GN=rpa34 PE=1 SV=1

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3009   933.4436 1864.8726 1864.8591 7.24 1 22 0.047 +1Score > 40 indicates identity
Score > 22 indicates homology
U R.QHFRPIGDAVGPESEPE.A + Deamidated (NQ)

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