MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 161–170 (out of 187)

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+161

Accession Score Description
1 DYHC_ANTCR 29 Dynein beta chain, ciliary OS=Anthocidaris crassispina PE=1 SV=1

+162

Accession Score Description
1 MREB_BACSU 29 Rod shape-determining protein mreB OS=Bacillus subtilis GN=mreB PE=3 SV=3

+163

Accession Score Description
1 HUTI_KLEP3 28 Imidazolonepropionase OS=Klebsiella pneumoniae (strain 342) GN=hutI PE=3 SV=1

+164

Accession Score Description
1 LPXC_AZOVD 28 UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=lpxC PE=3 SV=1

+165

Accession Score Description
1 SECA_HAEPS 28 Protein translocase subunit secA OS=Haemophilus parasuis serovar 5 (strain SH0165) GN=secA PE=3 SV=1

+166

Accession Score Description
1 COAX_ACICJ 27 Type III pantothenate kinase OS=Acidiphilium cryptum (strain JF-5) GN=coaX PE=3 SV=1

+167

Accession Score Description
1 HEAT1_DICDI 27 HEAT repeat-containing protein 1 homolog OS=Dictyostelium discoideum GN=heatr1 PE=3 SV=1

+168

Accession Score Description
1 OXAA_RUTMC 26 Inner membrane protein oxaA OS=Ruthia magnifica subsp. Calyptogena magnifica GN=oxaA PE=3 SV=1

+169

Accession Score Description
1 F16A2_ACAM1 26 Fructose-1,6-bisphosphatase class 1 2 OS=Acaryochloris marina (strain MBIC 11017) GN=fbp2 PE=3 SV=1

-170

Accession Score Description
1 RAPA_PSEPG 26 RNA polymerase-associated protein rapA OS=Pseudomonas putida (strain GB-1) GN=rapA PE=3 SV=1
Score Mass Matches Sequences emPAI
170.1 RAPA_PSEPG 26 106249 6 (1) 2 (1) 0.02
RNA polymerase-associated protein rapA OS=Pseudomonas putida (strain GB-1) GN=rapA PE=3 SV=1
8 samesets of RAPA_PSEPG
RAPA_PSEPK 26 106215 6 (1) 2 (1) 0.02
RNA polymerase-associated protein rapA OS=Pseudomonas putida (strain KT2440) GN=rapA PE=3 SV=1
RAPA_PSEPW 26 106243 6 (1) 2 (1) 0.02
RNA polymerase-associated protein rapA OS=Pseudomonas putida (strain W619) GN=rapA PE=3 SV=1
RAPA_PSEP1 26 106245 6 (1) 2 (1) 0.02
RNA polymerase-associated protein rapA OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rapA PE=3 SV=1
RAPA_PSEE4 26 105980 6 (1) 2 (1) 0.02
RNA polymerase-associated protein rapA OS=Pseudomonas entomophila (strain L48) GN=rapA PE=3 SV=1
RAPA_PSEAE 26 107034 1 (1) 1 (1) 0.02
RNA polymerase-associated protein rapA OS=Pseudomonas aeruginosa GN=rapA PE=3 SV=1
RAPA_PSEFS 26 106950 1 (1) 1 (1) 0.02
RNA polymerase-associated protein rapA OS=Pseudomonas fluorescens (strain SBW25) GN=rapA PE=3 SV=1
RAPA_PSEF5 26 106513 1 (1) 1 (1) 0.02
RNA polymerase-associated protein rapA OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rapA PE=3 SV=1
RAPA_PSEA8 26 107038 1 (1) 1 (1) 0.02
RNA polymerase-associated protein rapA OS=Pseudomonas aeruginosa (strain LESB58) GN=rapA PE=3 SV=1

-6 peptide matches (3 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
486 +1 702.3802 701.3729 701.3708 3.04 1 39 0.13 +1Score > 45 indicates identity
Score > 42 indicates homology		 E.LLDEGR.L
487 +2 351.6938 701.3730 701.3708 3.22 1 28 0.31 +1Score > 45 indicates identity
Score > 35 indicates homology		 E.LLDEGR.L
2122   710.3940 1418.7734 1418.7769 -2.46 0 26 0.028 +1Score > 39 indicates identity
Score > 23 indicates homology		 U R.LLTVLYPATGDTR.Q
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